Skip to main content
Log in

Electronic platelet counting with capillary blood samples—Experiences in a paediatric clinic

  • Published:
European Journal of Pediatrics Aims and scope Submit manuscript

Abstract

For several reasons, electronic platelet counts have not been generally adopted in paediatric routine laboratories. A method of platelet counting in capillary blood samples using an electronic counter is described. This is based on brief centrifugation of the blood sample after the addition of a diluent containing heparin/dextran and with a density of 1.050. The thrombocytes are uniformly suspended in the supernatant after 5 min of centrifugation at 250 g, so that the counting procedure in the electric unit can follow immediately. This simplified, dependable and reproducible method is also suitable for venous blood, and as a result of several years of successful trial, it can be recommended for routine use, especially in clinics with paediatric patients.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Besterman, E. M. M., Gillet, M. P. T.: Heparin effects on irreversible platelet aggregation. Lancet 1972 II, 282–283

  2. Brecher, G. Schneiderman, M., Cronkite, E. P.: The reproducibility and constancy of the platelet count. Amer. J. Clin. Path. 23, 15–26 (1953)

    Google Scholar 

  3. Bull, B. S., Schneiderman, M. A., Brecher G.: Platelet counts with the Coulter Counter. Amer. J. Clin. Path. 44 678–688 (1965)

    Google Scholar 

  4. Coulter, W. H.: High speed automatic blood cell counter and cell size analyzer. National Electronics Conference, Chicago, Ill. (1956)

  5. Davis, R. E., Green, R. E.: Automatic platelet counting with Coulter particle counter. J. clin. Path. 20, 777–779 (1967)

    Google Scholar 

  6. Day, H. J., Holmsen, H., Zucker, M. D.: Methods for separating platelets from blood and plasma. Thromb. Diath. Haemorrh. 33, 648–654 (1975)

    Google Scholar 

  7. Dhall, D. P., Matheson, N. A.: In vitro clumping of human platelets by dextran. Thromb. Diathes. Haemorrh. 19, 70–76 (1963)

    Google Scholar 

  8. Eastham, R. D.: Rapid whole-blood platelet counting using an electronic particle counter. J. clin. Path. 16, 168–169 (1963)

    Google Scholar 

  9. Eastham, R. D.: Simple method for separating platelets from red cells before enumeration with an electronic counter. J. clin. Path. 18, 248–249 (1965)

    Google Scholar 

  10. Eastham, R. D., Morgan, E. H.: Comparison of platelet count methods. The partial thrombolytic action of common platelet diluents demonstrated by electronic and visual counting methods. J. med. Lab. Tech. 24, 315–321 (1967)

    Google Scholar 

  11. Eggleton, M. J., Sharp, A. A.: Platelet counting using the Coulter electronic counter. J. clin. Path. 16, 164–167 (1963)

    Google Scholar 

  12. Feissly, R., Lüdin, H.: Microscopie par contrastes des phases. Rev. Hémat. 4, 481–501 (1949)

    Google Scholar 

  13. Fry, G. L., Hoak, J. C.: Improved method for electronic counting of platelets. J. Lab. Clin. Med. 74, 536–540 (1964)

    Google Scholar 

  14. Gadner, H., Odenwald, E., Plüss, H. J., Hitzig, W. H.: Thrombozytenzählung mit elektronischem Zählgerät im hämatologischen Routinelabor. Schweiz. med. Wschr. 104, 138–140 (1970)

    Google Scholar 

  15. Girling, J. H.: An automatic platelet counting technique. J. med. Lab. Tech. 19, 168–173 (1962)

    Google Scholar 

  16. Grebe, D., Augustin, I., Wellmer, H.-K.: Eine Methode zur Zählung von Thrombozyten im Blut mit einem automatischen Partikelzählgerät. Blut 19, 202–207 (1969)

    Google Scholar 

  17. Hellemann, P. W., v. Zwet, J. L., Geleisjnse, M. E. M.: Electronic counting of erythrocytes, leukocytes and platelets. In: Standardization in Haematology. III. Bibl. haemat. (de Boroviszény, Ch. G., ed.), Vol. 24, pp. 54–57. Basel, New York: Karger 1966

    Google Scholar 

  18. Hitzig, W. H.: Neue Methode zur Thrombozytenisolierung. Schweiz. med. Wschr. 39, 1126–1127 (1954)

    Google Scholar 

  19. Höffler, U., Oette, K.: Manuelle und mechanisierte Zähltechniken zur Bestimmung der Thrombozyten. Med. Lab. 28, 6–17 (1975)

    Google Scholar 

  20. Morgan, M. C., Szafib, J. J.: Separation of platelets from whole blood by the use of Silicone liquids. Blood 18, 89–94 (1961)

    Google Scholar 

  21. Sipe, C. R., Cronkite, E. P.: Studies on the application of the Coulter electronic counter in enumeration of platelets. Ann. N.Y. Acad. Sci. 99, 262–270 (1962)

    Google Scholar 

  22. Tullis, J. L.: Separation and purification of leukocytes and platelets. Blood 7, 891–896 (1952)

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gadner, H., Odenwald, E., Riehm, H. et al. Electronic platelet counting with capillary blood samples—Experiences in a paediatric clinic. Eur J Pediatr 129, 37–45 (1978). https://doi.org/10.1007/BF00441372

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00441372

Key words

Navigation