Abstract
For several reasons, electronic platelet counts have not been generally adopted in paediatric routine laboratories. A method of platelet counting in capillary blood samples using an electronic counter is described. This is based on brief centrifugation of the blood sample after the addition of a diluent containing heparin/dextran and with a density of 1.050. The thrombocytes are uniformly suspended in the supernatant after 5 min of centrifugation at 250 g, so that the counting procedure in the electric unit can follow immediately. This simplified, dependable and reproducible method is also suitable for venous blood, and as a result of several years of successful trial, it can be recommended for routine use, especially in clinics with paediatric patients.
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References
Besterman, E. M. M., Gillet, M. P. T.: Heparin effects on irreversible platelet aggregation. Lancet 1972 II, 282–283
Brecher, G. Schneiderman, M., Cronkite, E. P.: The reproducibility and constancy of the platelet count. Amer. J. Clin. Path. 23, 15–26 (1953)
Bull, B. S., Schneiderman, M. A., Brecher G.: Platelet counts with the Coulter Counter. Amer. J. Clin. Path. 44 678–688 (1965)
Coulter, W. H.: High speed automatic blood cell counter and cell size analyzer. National Electronics Conference, Chicago, Ill. (1956)
Davis, R. E., Green, R. E.: Automatic platelet counting with Coulter particle counter. J. clin. Path. 20, 777–779 (1967)
Day, H. J., Holmsen, H., Zucker, M. D.: Methods for separating platelets from blood and plasma. Thromb. Diath. Haemorrh. 33, 648–654 (1975)
Dhall, D. P., Matheson, N. A.: In vitro clumping of human platelets by dextran. Thromb. Diathes. Haemorrh. 19, 70–76 (1963)
Eastham, R. D.: Rapid whole-blood platelet counting using an electronic particle counter. J. clin. Path. 16, 168–169 (1963)
Eastham, R. D.: Simple method for separating platelets from red cells before enumeration with an electronic counter. J. clin. Path. 18, 248–249 (1965)
Eastham, R. D., Morgan, E. H.: Comparison of platelet count methods. The partial thrombolytic action of common platelet diluents demonstrated by electronic and visual counting methods. J. med. Lab. Tech. 24, 315–321 (1967)
Eggleton, M. J., Sharp, A. A.: Platelet counting using the Coulter electronic counter. J. clin. Path. 16, 164–167 (1963)
Feissly, R., Lüdin, H.: Microscopie par contrastes des phases. Rev. Hémat. 4, 481–501 (1949)
Fry, G. L., Hoak, J. C.: Improved method for electronic counting of platelets. J. Lab. Clin. Med. 74, 536–540 (1964)
Gadner, H., Odenwald, E., Plüss, H. J., Hitzig, W. H.: Thrombozytenzählung mit elektronischem Zählgerät im hämatologischen Routinelabor. Schweiz. med. Wschr. 104, 138–140 (1970)
Girling, J. H.: An automatic platelet counting technique. J. med. Lab. Tech. 19, 168–173 (1962)
Grebe, D., Augustin, I., Wellmer, H.-K.: Eine Methode zur Zählung von Thrombozyten im Blut mit einem automatischen Partikelzählgerät. Blut 19, 202–207 (1969)
Hellemann, P. W., v. Zwet, J. L., Geleisjnse, M. E. M.: Electronic counting of erythrocytes, leukocytes and platelets. In: Standardization in Haematology. III. Bibl. haemat. (de Boroviszény, Ch. G., ed.), Vol. 24, pp. 54–57. Basel, New York: Karger 1966
Hitzig, W. H.: Neue Methode zur Thrombozytenisolierung. Schweiz. med. Wschr. 39, 1126–1127 (1954)
Höffler, U., Oette, K.: Manuelle und mechanisierte Zähltechniken zur Bestimmung der Thrombozyten. Med. Lab. 28, 6–17 (1975)
Morgan, M. C., Szafib, J. J.: Separation of platelets from whole blood by the use of Silicone liquids. Blood 18, 89–94 (1961)
Sipe, C. R., Cronkite, E. P.: Studies on the application of the Coulter electronic counter in enumeration of platelets. Ann. N.Y. Acad. Sci. 99, 262–270 (1962)
Tullis, J. L.: Separation and purification of leukocytes and platelets. Blood 7, 891–896 (1952)
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Gadner, H., Odenwald, E., Riehm, H. et al. Electronic platelet counting with capillary blood samples—Experiences in a paediatric clinic. Eur J Pediatr 129, 37–45 (1978). https://doi.org/10.1007/BF00441372
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DOI: https://doi.org/10.1007/BF00441372