Abstract
We had an outbreak of acute infantile gastroenteritis accompanied by milky-white stool (called Hakuri in Japanese) during the winters of 1976 and 1977. Stool specimens collected from 72 cases of Hakuri were studied by negative-staining electron microscopy. Rotavirus was detected with a very high frequency (89%).
Rotavirus obtained from one of the patients was isolated and passaged in cultures of primary human embryonic kidney cells. Viral antigens could be detected in the cytoplasm of the cells by indirect immuno-fluorescence. The fluorescence-positive cells increased in number with repeated passage.
Serum anti-viral activities in 11 patients were titrated by indirect immuno-fluorescence, using the cells infected with the passaged rotavirus. All 11 patients developed IgM responses in the convalescent phase. However, in 4 of the 11 patients, no IgG responses were detected even 2–3 weeks after the onset of illness. The reinfection which has occasionally be seen in our country may be related to these poor IgG responses.
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References
Bishop, R. F., Davidson, G. P., Holmes, I. H., Ruck, B. J.: Detection of a new virus by electron microscopy of fecal extracts from children with acute gastroenteritis. Lancet 1974I, 149–151
Fanconi, G., Wallgren, A.: Lehrbuch der Pädiatrie, 8th ed., p. 117. Basel-Stuttgart: Schwabe 1969
Flewett, T. H., Bryden, A. S., Davies, H.: Virus particles in gastroenteritis. Lancet 1973I, 1497
Frederick, G. T., Bohl, E. H.: Local and systemic cell-mediated immunity against transmissible gastroenteritis; an intestinal viral infection of swine. J. Immunol. 116, 1000–1004
Kapikian, A. Z., Kim, H. W., Wyatt, R. G., Cline, W. L. Arrobio, J. O., Brandt, C. D., Rodriguez, W. J., Sack, D. A., Chanock, R. M., Parrot, R. H.: Human reovirus-like agent as the major pathogen associated with winter gastroenteritis in hospitalized infants and young children. N. Engl. J. Med. 294, 965–972 (1976)
Kawamura, A.: Fluorescent antibody techniques and their applications, 1st ed. Tokyo-Baltimore-Manchester: University of Tokyo Press and University Park Press 1969
Konno, T., Suzuki, H., Ishida, N.: Reovirus-like agent in Japanese infants with gastroenteritis. Lancet 1975I, 918–919
Kruse, P. F., Patterson, M. K.: Tissue culture-methods and applications, 1st ed. New York-San Francisco-London: Academic Press 1973
Martin, L. M., Palmer, E. L., Middleton, P. J.: Ultrastructure of infantile gastroenteritis virus. Virology 68, 146–153 (1975)
Matsuno, S., Inoue, S., Kono, R.: Plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera. J. Clin. Microbiol. 5, 1–4 (1977)
Mebus, C. A., Wyatt, R. G., Sharpee, R. L., Sereno, M. M., Kalica, A. R., Kapikian, A. Z., Twiehaus, M. J.: Diarrhea in gnotobiotic calves by the reovirus-like agent of human infantile gastroenteritis. Infect. Immun. 14, 471–474 (1976)
Middleton, P. J., Petric, M., Szymanski, M. T.: Propagation of infantile gastroenteritis (orbigroup) in conventional germfree piglets. Infect. Immun. 12, 1276–1280 (1975)
Middleton, P. J., Szymansky, M. T., Abbott, G. D., Bortolussi, R., Hamilton, J. R.: Orbivirus acute gastroenteritis of infancy, Lancet 1974II, 1241
Morishima, T., Nagayoshi, S., Ozaki, T., Isomura, S., Suzuki, S.: Immuno fluorescence of human reovirus-like agent of infantile diarrhea. Lancet 1976 II, 695–696
Sakamoto, A., Iijima, S., Suzuki, S., Shirasawa, T., Mori, Y., Niimi, M.: Clinical and Pathological studies on Hakuri. Ann. Pediat. 202, 293–302 (1964)
Wyatt, R. G., Gill, V. W., Sereno, M. M., Kalica, A. R., Vankirk, D. H., Chanock, R. M., Kapikian, A. Z.: Propable in-vitro cultivation of human reovirus-like agent of infantile diarrhea. Lancet 1976 I, 98–99
Yamashita, F., Shibuya, S., Townley, R. R. W., Davidson, G. P., Bishop, R. F., Holmes, I. H., Ruck, B. J.: Duovirus in pseudocholera infantum. Lancet 1975I, 1297
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Morishima, T., Ichikawa, T., Yamaguchi, H. et al. Acute infantile gastroenteritis caused by rotavirus in Japan. Eur J Pediatr 129, 259–265 (1978). https://doi.org/10.1007/BF00441356
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DOI: https://doi.org/10.1007/BF00441356