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Supersensitive double mutants in yeast

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Summary

Double mutants containing the radiosensitive alleles uxsl (UVR-EXR-) and either uvsl-2 or uvsl 9-2 (both UVR-EXR+) have been constructed. We find that these double mutants are extremely sensitive to UV: their LD37 doses are 0.3–0.5 ergs/mm2 as compared to 16–40 ergs/mm2 for the radiosensitive parental strains carrying only a single mutant gene and 456 ergs/mm2 for wild-type.

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References

  • Brendel, M., Khan, N. A., Haynes, R. H.: Common steps in the repair of alkylation and radiation damage in yeast. Molec. Gen. Genetics 106, 289–295 (1970).

    Google Scholar 

  • Johnson, J. R., Mortimer, R. K.: Use of snail digestive juice in isolation of yeast tetrads. J. Bact. 78, 292 (1959).

    Google Scholar 

  • Howard-Flanders, P., Boyce, R. P.: DNA repair and genetic recombination: Studies on mutants of Escherichia coli defective in these processes. Radiat. Res., Suppl. 6, 156–184 (1966).

    Google Scholar 

  • —, Theriot, L.: Mutants of Escherichia coli, K-12 defective in DNA repair and in genetic recombination. Genetics 53, 1137–1150 (1966).

    Google Scholar 

  • Resnick, M. A.: Genetic control of lethality and mutation in Saccharomyces cerevisiae. Ph. d. Thesis, Univ. of California, UCRL-18404 (1968).

  • Witkin, E. M.: Mutability towards ultraviolet light of recombination — deficient strains of Escherichia coli. Mutation Res. 8, 9–14 (1969).

    Google Scholar 

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Authors and Affiliations

  1. Department of Biology, York University, Toronto, Canada

    N. A. Khan, M. Brendel & R. H. Haynes

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  1. N. A. Khan
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  2. M. Brendel
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  3. R. H. Haynes
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Communicated by E. Witkin

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Khan, N.A., Brendel, M. & Haynes, R.H. Supersensitive double mutants in yeast. Molec. Gen. Genet. 107, 376–378 (1970). https://doi.org/10.1007/BF00441200

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  • DOI: https://doi.org/10.1007/BF00441200

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