Molecular and General Genetics MGG

, Volume 107, Issue 4, pp 291–304 | Cite as

Ultraviolet-sensitive mutants of Neurospora

I. Genetic basis and effect on recombination
  • Alice Louise Schroeder
Article

Summary

Three new UV-sensitive mutants were obtained using replica plating of the colonial crisp ragged strain of Neurospora crassa — uvs-3 (near cys-10, linkage group IVL), uvs-4 (4 units left of ad-4 IIIR) and uvs-5 (<1 unit from vel, IIIR). These are genetically distinct from uvs-1 (Chang and Tuveson, 1967) and uvs-2 (IVR, Stadler and Smith, 1968). They are two to three times more sensitive to UV than wild type. Heterokaryons between any two mutations are not sensitive, showing that all three are recessive. In heterozygous condition, none of the mutants affects crossing over. In homozygous condition, uvs-4 does not affect gene conversion as measured by prototroph frequency in crosses of pan-2 (B2) x pan-2 (B36), nor does it affect crossing over between cot-1 and tryp-4. Neither uvs-3 nor uvs-5 is fertile in homozygous crosses; asci do not develop beyond the multinucleate ascogenous hypha stage. — Mitotic effects were studied using strains haploid for UV-sensitivity but duplicated and heterozygous for mating type. In(ILR) H 4250 and Tp(I→II) 39311 were used to generate such duplications. Release from a slow-growing phenotype occurs when a mitotic event makes mating type homo-or hemizygous. With uvs-3, but not uvs-4 or uvs-5, release occurs 2 days earlier than in controls. Thus uvs-3 may affect chromosome breakage or mitotic recombination. The number of chromosome rearrangements present in a sample of colonies grown from single conidia of uvs-3 stocks is the same as in controls, but in test crosses 13% of the colonies produced barren perithecia of a type that is characteristic of duplications.

Keywords

Recombination Linkage Group Ascus Mating Type Chromosome Rearrangement 

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References

  1. Bachmann, B. J., Strickland, W. N.: Neurospora bibliography and index. New Haven, Conn.: Yale University Press 1965.Google Scholar
  2. Barratt, R. W., Ogata, W. N.: Neurospora stock list. Neurospora Newsl. 13, 25–95 (1968).Google Scholar
  3. Barry, E. G.: Cytological techniques for meiotic chromosomes in Neurospora. Neurospora Newsl. 10, 12–13 (1966).Google Scholar
  4. —: Chromosome aberrations in Neurospora, and the correlation of chromosomes and linkage groups. Genetics 55, 21–32 (1968).Google Scholar
  5. Brockman, H. E., Serres, F. J. de: Sorbose toxicity in Neurospora. Amer. J. Bot. 50, 709–714 (1963).Google Scholar
  6. Case, M. E., Giles, N. H.: Recombination mechanisms at the pan-2 locus in Neurospora crassa. Cold Spr. Harb. Symp. quant. Biol. 23, 119–135 (1958).Google Scholar
  7. Chang, L. T., Tuveson, R. W.: Ultraviolet-sensitive mutants in Neurospora crassa. Genetics 56, 801–810 (1967).Google Scholar
  8. Clark, A. J., Margulies, A. D.: Isolation and characterization of recombination-deficient mutants of Escherichia coli K-12. Proc. nat. Acad. Sci. (Wash.) 53, 451–459 (1965).Google Scholar
  9. Cox, B. S., Parry, J. M.: The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast. Mutation Res. 6, 37–55 (1968).Google Scholar
  10. Davies, D. R.: UV-sensitive mutants of Chlamydomonas reinhardi. Mutation Res. 4, 765–770 (1967).Google Scholar
  11. Enns, H., Larter, E. N.: Linkage relations of ds: a gene governing chromosome behavior in barley and its effect on genetic recombination. Canad. J. Genet. Cytol. 4, 263–266 (1962).Google Scholar
  12. Gowen, J. W.: Meiosis as a genetic character in Drosophila melanogaster. J. exp. Zool. 65, 83–106 (1933).Google Scholar
  13. Haefner, K.: Spontaneous lethal sectoring, a further feature of Escherichia coli strains deficient in the function of rec and uvr genes. J. Bact. 96, 652–659 (1968).Google Scholar
  14. — Howrey, L.: Gene controlled UV-sensitivity in Schizosaccharomyces pombe. Mutation Res. 4, 219–221 (1967).Google Scholar
  15. Holliday, R.: Radiation sensitive mutants of Ustilago maydis. Mutation Res. 2, 557–559 (1965).Google Scholar
  16. —: Altered recombination frequencies in radiation sensitive strains of Ustilago. Mutation Res. 4, 275–288 (1967).Google Scholar
  17. — Genetic recombination in fungi. In: Replication and recombination of genetic material (W. J. Peacock and R. D. Brock, eds.), p. 157–174. Canberra: Australian Acad. Sci. 1968.Google Scholar
  18. Howard-Flanders, P.: DNA repair. Ann. Rev. Biochem. 37, 175–200 (1968).Google Scholar
  19. — Theriot, L.: Mutants of Escherichia coli K-12 defective in DNA repair and in genetic recombination. Genetics 53, 1137–1150 (1966).Google Scholar
  20. Lanier, W. B., Tuveson, R. W., Lennox, J. E.: A radiation-sensitive mutant of Aspergillus nidulans. Mutation Res. 5, 23–31 (1968).Google Scholar
  21. Lederberg, J., Lederberg, E.: Replica plating and indirect selection of bacterial mutants. J. Bact. 63, 399–406 (1952).Google Scholar
  22. Lein, J., Mitchell, H. K., Houlahan, M. B.: A method of selection of biochemical mutants of Neurospora. Proc. nat. Acad. Sci. (Wash.) 34, 435–442 (1948).Google Scholar
  23. Lennox, J.E., Tuveson, R. W.: The isolation of ultraviolet-sensitive mutants from Aspergillus rugoulosus. Radiat. Res. 31, 382–388 (1967).Google Scholar
  24. Low, B.: Formation of merodiploids in matings with a class of rec - recipient strains of E. coli K-12. Proc. nat. Acad. Sci. (Wash.) 60, 160–167 (1968).Google Scholar
  25. Maling, B. D.: Replica plating and rapid ascus collection of Neurospora. J. gen. Microbiol. 23, 257–260 (1960).Google Scholar
  26. Nakai, S., Matsumoto, S.: Two types of radiation-sensitive mutant in yeast. Mutation Res. 4, 129–136 (1967).Google Scholar
  27. Newmeyer, D., Taylor, C. W.: A pericentric inversion in Neurospora with unstable duplication progeny. Genetics 56, 771–791 (1967).Google Scholar
  28. Nga, B. H., Roper, J. A.: Quantitative intrachromosomal changes arising at mitosis in Aspergillus nidulans. Genetics 58, 193–209 (1968).Google Scholar
  29. Perkins, D. D.: New markers and multiple point linkage data in Neurospora. Genetics 44, 1185–1208 (1959).Google Scholar
  30. —: An insertional translocation in Neurospora that generates duplications heterozygous for mating type. Genetics 54, 354 (Abstr.) (1966a).Google Scholar
  31. —: Details for collection of asci as unordered groups of eight projected ascospores. Neurospora Newsl. 9, 11 (1966b).Google Scholar
  32. Perkins, D. D.: An insertional translocation in Neurospora that generates duplications heterozygous for mating type. (1970) (in preparation).Google Scholar
  33. — Glassey, M., Bloom, B. S.: New data on markers and rearrangements in Neurospora. Canad. J. Genet. Cytol. 4, 187–205 (1962).Google Scholar
  34. Perkins, D. D., Newmeyer, D., Taylor, C. W., Bennett, D. C.: New markers and map sequences in Neurospora crassa with a description of mapping by duplication coverage and of multiple translocation stocks for testing linkage. Genetica 40 (1969).Google Scholar
  35. Pittenger, T. H.: Distribution of nuclei in conidia. Neurospora Newsl. 11, 10–12 (1967).Google Scholar
  36. Puglisi, P. P.: Genetic control of radiation sensitivity in yeast. Radiat. Res. 31, 856–866 (1967).Google Scholar
  37. Radford, A.: Variability of recombination within the pyridoxine locus of Neurospora. Canad. J. Genet. Cytol. 10, 444–447 (1968).Google Scholar
  38. Resnick, M. A.: Genetic control of lethality and mutation in Saccharomyces cerevisiae. Doctoral dissertation, University of California, Berkeley (1968).Google Scholar
  39. Riley, R., Law, C. N.: Genetic variation in chromosome pairing. Advanc. Genet. 13, 57–114 (1965).Google Scholar
  40. —, Miller, T. E.: The differential sensitivity of desynaptic and normal genotypes of barley to X-rays. Mutation Res. 3, 355–359 (1966).Google Scholar
  41. Schroeder, A. L.: Ultraviolet-sensitive mutants in Neurospora crassa. Genetics 60, 223 (Abstr.) (1968).Google Scholar
  42. Schroeder, A. L.: UV-sensitive mutants in Neurospora. II. Radiation studies. Molec. gen. Genetics. (1970).Google Scholar
  43. Shanfield, B., Kafer, E.: UV-sensitive mutants increasing mitotic crossing-over in Aspergillus nidulans. Mutation Res. 7, 485–487 (1969).Google Scholar
  44. Siegel, S.: Nonparametric statistics for the behavioral sciences, p. 116. New York: McGraw-Hill 1956.Google Scholar
  45. Smith, P. A., King, R. C.: Genetic control of synaptonemal complexes in Drosophila melanogaster. Genetics 60, 335–351 (1968).Google Scholar
  46. Snow, R.: Recombination in ultraviolet-sensitive strains of Saccharomyces cerevisiae. Mutation Res. 6, 409–418 (1968).Google Scholar
  47. Stadler, D. R., Smith, D. A.: A new mutation in Neurospora for sensitivity to ultraviolet. Canad. J. Genet. Cytol. 10, 916–919 (1968).Google Scholar
  48. —, Towe, A. M.: Genetic factors influencing crossing-over in Neurospora. Genetics 47, 839–846 (1962).Google Scholar
  49. Stevens, W. L.: Accuracy of mutation rates. J. Genetics 43, 301–307 (1942).Google Scholar
  50. Strickland, W. N.: A rapid method for obtaining unordered Neurospora tetrads. J. gen. Microbiol. 22, 583–588 (1960).Google Scholar
  51. Tatum, E. L., Barratt, R. W., Fries, N., Bonner, D.: Biochemical mutant strains of Neurospora produced by physical and chemical treatment. Amer. J. Bot. 37, 38–46 (1950).Google Scholar
  52. Taylor, C. W.: A more efficient procedure for scoring mating type and aberrations. Neurospora Newsl. 8, 21 (1965).Google Scholar
  53. Vigfusson, N. V.: Sterility mutants in Neurospora crassa. Canad. J. Genet. Cytol. 11, 483 (Abstr.) (1969).Google Scholar
  54. Vogel, H. J.: Distribution of lysine pathways among fungi: evolutionary implications. Amer. Naturalist 98, 435–446 (1964).Google Scholar
  55. Watson, W. A. F.: Studies on a recombination-deficient mutant of Drosophila. I. Dominant lethals. Mutation Res. 8, 91–100 (1969).Google Scholar
  56. Westergaard, M., Mitchell, H. K.: Neurospora. V. A synthetic medium favouring sexual reproduction. Amer. J. Bot. 34, 573–578 (1947).Google Scholar
  57. Witkin, E. M.: Mutation-proof and mutation-prone modes of survival in derivatives of Escherichia coli B differing in sensitivity to ultraviolet light. Brookhaven Symp. Biol. 20, 17–55 (1968).Google Scholar
  58. Zakharov, I. A., Kozhina, T. N.: Yeast mutant supersensitive to ultraviolet light. Dokl. Acad. Nauk SSSR Biol. Sci. Sect. (English Trans.) 176, 605–606 (1967).Google Scholar

Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • Alice Louise Schroeder
    • 1
    • 2
  1. 1.Department of Biological SciencesStanford UniversityStanford
  2. 2.Department of Molecular BiophysicsWashington State UniversityPullman

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