European Journal of Pediatrics

, Volume 137, Issue 1, pp 27–29 | Cite as

The Rose Bengal test in neonatal cholestasis: Diagnostic and prognostic value

  • F. Alvarez
  • J. Yvart
  • M. Odièvre
Original Investigations


131I Rose Bengal (131IRB) studies were performed in 73 infants with extrahepatic biliary atresia (EHBA) and in 37 with intrahepatic cholestasis of various origins. Fecal 131IRB excretion of less than 10% (“complete” cholestasis) was observed in EHBA but also in some patients with either paucity of intrahepatic bile ducts (syndromatic type) or with α-1-antitrypsin deficiency. Seventy one 131IRB tests were also performed 3 to 8 weeks postoperatively in children operated on for EHBA. Fecal 131IRB excretion more than 15% was present in 27 out of 34 cases who were later completely jaundice free and in only one out of 37 cases where no bile flow restoration occurred.

These results indicate that complete cholestasis in infants can be observed in some types of intrahepatic cholestasis, as well as in EHBA, and show that a postoperative 131IRB test is a reliable means of predicting complete restoration of bile flow in EHBA.

Key words

Neonatal cholestasis Rose Bengal test 


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  1. 1.
    Alagille D, Odièvre, M, Gautier M, Dommergues JP (1975) Hepatic ductular hypoplasia associated with characteristic facies, vertebral malformations, retarded physical, mental and sexual development and cardiac murmur. J pediat 86:63–71Google Scholar
  2. 2.
    Desbuquois B, Tron Ph, Alagille D (1968) Etude de l'excrétion fécale et urinaire du Rose Bengale marqué par l'iode radioactif. Arch franç Pédiat 25:379–391Google Scholar
  3. 3.
    Gautier M, Jehan P, Odièvre M (1976) Histological study of biliary fibrous remnants in 48 cases of extrahepatic biliary atresia. Correlation with postoperative bile flow restoration. J Pediat 89:704–709Google Scholar
  4. 4.
    Ghadimi H, Sass-Kortsak A (1961) Evaluation of radioactive Rose Bengal test for the differential diagnosis of obstructive jaundice in infants. N Engl J Med 265:351–358Google Scholar
  5. 5.
    Kasai M (1974) Treatment of biliary atresia with special reference to hepatic porto-enterostomy and its modifications. Progr Pediatr Surg 6:5–52Google Scholar
  6. 6.
    Maksoud JG, Thom AF, Kieffer J, Carvalho Pinto VA (1971) Fecal excretion of Rose Bengal I131 in the diagnosis of obstructive jaundice in infancy with special reference to biliary atresia. Pediatrics 48:966–969Google Scholar
  7. 7.
    Meurman L (1960) On the distribution and kinetics of injected I131 Rose Bengal. Acta Med Scand Suppl 354Google Scholar
  8. 8.
    Odièvre M, Martin JP, Hadchouel M, Alagille D (1976) Alpha-1-antitrypsin deficiency and liver diseases in children: phenotypes, manifestations and prognosis. Pediatrics 57:226–231Google Scholar
  9. 9.
    Odièvre M, Valayer J, Razemon-Pinta M, Habib EC, Alagille D (1976) Hepatic porto-enterostomy or cholecystostomy in the treatment of extra-hepatic biliary atresia. J Pediat 88:774–779Google Scholar
  10. 10.
    Pirotte J (1979) Suitability criteria for comportamental analysis of the plasma clearance curve of exogenous cholephils. 131I Rose Bengal fulfils these criteria. Biomedicine 30:211–215Google Scholar
  11. 11.
    Poley RJ, Alaupovic P, McConathy WJ, Seidel D, Roy C, Weber A (1973) Diagnosis of extrahepatic biliary obstruction in infants by immunochemical detection of LP-X and modified 131I-Rose Bengal excretion test. J Lab Clin Med 81:325–341Google Scholar
  12. 12.
    Poley RJ, Smith E, Boon D, Bhatia M, Smith C, Thompson JB (1972) Lipoprotein-X and the double 131I Rose Bengal test in the diagnosis of prolonged infantile jaundice. J Ped Surgery 7:660–669Google Scholar
  13. 13.
    Thaler M (1976) Cryptogenic liver disease in young infants. In: Popper H, Schaffner F (eds) Progress in liver diseases. Grune and Stratton, New York, Vol 5, pp 476–493Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • F. Alvarez
    • 1
  • J. Yvart
    • 2
  • M. Odièvre
    • 1
  1. 1.The Unité de Recherche d'Hepatologie Infantile INSERM U 56 et Clinique de PédiatrieUniversité Paris-Sud, Hôpital de BicêtreBicêtreFrance
  2. 2.The Service de Biophysique et Médecine NucléaireC.H.U. BicêtreBicêtreFrance

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