Mycopathologia

, Volume 86, Issue 3, pp 143–153 | Cite as

Ecology of nematophagous fungi: Methods of collection, isolation and maintenance of predatory and endoparasitic fungi

  • N. F. Gray
Article

Abstract

The methods employed in a recent investigation into the ecology of nematophagous fungi are examined in detail. The best sources from which fungi can be isolated are examined, and the main field collection and recovery techniques are compared and discussed. Other practical problems considered are the examination of plates, microscopy, identification, isolation and culture methods, maintenance of fungi, the production of nematodes as bait. A summary of the experimental techniques used is given.

Keywords

Microscopy Experimental Technique Practical Problem Culture Method Good Source 

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References

  1. 1.
    Aschner, M. & S. Kohn, 1958. The biology of Harposporium anguillulae. J. Gen. Microbiol. 19: 182–189.Google Scholar
  2. 2.
    Baermann, G., 1917. Eine einfache Methode zur Affindung von Ankylostomum (Nematoden) Larven in Enproben. Meded. Geneesk. Lab. Weltevreden, Feestbundel 41.Google Scholar
  3. 3.
    Balan, J., L. Krizkova, P. Nemec & A. Kolozsvary, 1976. A qualitative method for detection of nematode attracting substances and proof of production of three different attractants by the fungus Monacrosporium rutgeriense. Nematologica 22: 306–311.Google Scholar
  4. 4.
    Barron, G. L., 1969. Isolation and maintenance of endoparasitic nematophagous hyphomycetes. Can. J. Bot. 47: 1899–1902.Google Scholar
  5. 5.
    Barron, G. L., 1977. The Nematode-Destroying Fungi. Topics in Mycobiology 1. Canadian Biological Publications, Ontario.Google Scholar
  6. 6.
    Barron, G. L., 1977. Nematophagous fungi: Protascus and its relationship to Myzocytium. Can. J. Bot. 55: 819–824.Google Scholar
  7. 7.
    Barron, G. L., 1978. Nematophagous fungi: endoparasites of Rhabditis terricola. Microb. Ecol. 4: 157–163.Google Scholar
  8. 8.
    Barron, G. L., 1979. Observations of predatory fungi. Can. J. Bot. 57: 187–193.Google Scholar
  9. 9.
    Capstick, C. K., D. C. Twinn & J. S. Waid, 1957. Predation of natural populations of free-living nematodes by fungi. Nematologia 2: 193–201.Google Scholar
  10. 10.
    Cooke, R. C., 1961. Agar disc method for the direct observation of nematode-trapping fungi in the soil. Nature Lond. 191: 1411–1412.Google Scholar
  11. 11.
    Cooke, R. C., 1962. The ecology of nematode-trapping fungi in the soil. Ann. Appl. Biol. 50: 507–513.Google Scholar
  12. 12.
    Cooke R. C. & C. H. Dickinson, 1965. Nematode-trapping species of Dactylella and Monacrosporium. Trans. Brit. mycol. Soc. 48: 621–629.Google Scholar
  13. 13.
    Cooke, R. C. & B. E. S. Godfrey, 1964. A key to the nematode-destroying fungi. Trans. Brit. mycol. Soc. 47: 61–74.Google Scholar
  14. 14.
    Dixon, S. M. & Duddington, C. L., 1951. Permanent preparation of fungi growing on agar. Nature Lond. 168: 38–39.Google Scholar
  15. 15.
    Dollfus, R. P., 1946. Parasites (animaux et vegetaux) des Helminthes. Paul Lechevalier, Paris.Google Scholar
  16. 16.
    Dowsett, J, A. J. Reid & A. Hopkin, 1982. On Cephalosporium balanoides Drechsler. Mycologia 74: 687–690.Google Scholar
  17. 17.
    Dougherty, E., 1960. Cultivation of Aschelminthes, especially Rhabditis nematodes. In J. N. Sasser & W. R. Jenkins (eds.), Nematology. University of North Carolina Press, Chapel Hill.Google Scholar
  18. 18.
    Duddington, C. L., 1951. The ecology of predaceous fungi. I: Preliminary survey. Trans. Brit. mycol. Soc. 34: 322–331.Google Scholar
  19. 19.
    Duddington, C. L., 1955. Notes on the technique of handling predaceous fungi. Trans. Brit. mycol. Soc. 38: 97–103.Google Scholar
  20. 20.
    Duddington, C. L., 1962. Predaceous fungi and the control of eelworms. Viewpoints Biol. 1: 151–200.Google Scholar
  21. 21.
    Duddington, C. L. & C. H. E. Wyborn, 1972. Recent research on the nematophagous hyphomycetes. Bot. Rev. 38: 545–565.Google Scholar
  22. 22.
    Eren, J. & D. Pramer, 1965. The most probable number of nematode-trapping fungi in soil. Soil Sci. 99: 285.Google Scholar
  23. 23.
    Feder, W. A., 1963. A comparison of nematode-capturing efficiencies of five Dactylella species at four temperatures. Mycopathol. Mycol. appl. 19: 99–104.Google Scholar
  24. 24.
    Feder, W. A., C. O. R. Everard & L. M. O. Wooton, 1963. The sensitivity of several species of the nematophagous fungi Dactylella to a morphogenic substance derived from free-living nematodes. Nematologica 9: 49–54.Google Scholar
  25. 25.
    Fowler, M., 1970. New Zealand predaceous fungi. N. Z. J. Bot. 8: 283–302.Google Scholar
  26. 26.
    Gams, W. & A. van Zaayen, 1982. Contribution to the taxonomy and pathogenicity of fungicolous Verticillium species. I: Taxonomy. Neth. J. Plant Pathol. 88: 57–78.Google Scholar
  27. 27.
    Giuma, A. Y. & R. C. Cooke, 1972. Some endozoic parasites on soil nematodes. Trans. Brit. mycol. Soc. 59: 213–218.Google Scholar
  28. 28.
    Goodey, J. B., 1963. Laboratory Methods for Work with Plant and Soil Nematodes. Tech. Bull. 2: Min. Agric. Fish Food, 4th ed. H.M.S.O., London.Google Scholar
  29. 29.
    Gray, N. F., 1982. Psychrotolerant nematophagous fungi from the maritime Antarctic. Plant Soil 64: 431–435.Google Scholar
  30. 30.
    Gray, N. F., 1983. Ecology of nematophagous fungi: distribution and habitat. Ann. appl. Biol. 102: 501–509.Google Scholar
  31. 31.
    Gray, N. F., 1984. Ecology of nematophagous fungi: recovery of endoparasites using the Baermann funnel technique. Soil Biol. Biochem. (in press).Google Scholar
  32. 32.
    Gray, N. G., 1984. Comparative morphology of Acrostalagmus obovatus and Verticillium sphaerosporum, two endoparasitic hyphomycetes of soil nematodes (in preparation).Google Scholar
  33. 33.
    Gray, N. F., 1983. Ecology of nematophagous fungi: notes on the identification of A crostalagmus obovatus Drechsler, Cephalosporium balanoides Drechsler and Verticillium sphaerosporum Goody. Ir. Nat. J. 21: 125–128.Google Scholar
  34. 34.
    Gray, N. F., 1983. Ecology of nematophagous fungi: Panangrellus redivivus as the target nematode. Plant Soil 73: 293–297.Google Scholar
  35. 35.
    Gray, N. F., 1983. Ecology of nematophagous fungi: data base. E.S.U. Consultancy Report: G15. Environmental Sciences Unit, Trinity College, Dublin. 43 pp.Google Scholar
  36. 36.
    Gray, N. F. & K. R. Duff, 1982. Some preliminary observations on predaceous fungi from Ireland. Ir. Nat. J. 20: 378–380.Google Scholar
  37. 37.
    Gray, N. F. & R. I. L. Smith, 1984. The distribution of nematophagous fungi from the maritime Antarctic. Mycopathologia 85: 81–92.Google Scholar
  38. 38.
    Gray, N. F., C. H. E. Wyborn& R. I. Lewis-Smith, 1982. An ecological study of nematophagous fungi from the maritime Antarctic. Oikos 38: 194–201.Google Scholar
  39. 39.
    Heintz, C. E., 1978. Assessing the predacity of nematodetrapping fungi in vitro. Mycologia 70: 1086–1100.Google Scholar
  40. 40.
    Heintz, C. E. & Pramer, D., 1972. Ultrastructure of nematode-trapping fungi. J. Bacteriol. 110: 1163–1170.Google Scholar
  41. 41.
    Jansson, H. B., 1982. Attraction of nematodes to endoparasitic nematophagous fungi. Trans. Brit. mycol. Soc. 79: 25–29.Google Scholar
  42. 42.
    Jansson, H. B. & B. Nordbring-Hertz, 1979. Attraction of nematodes to living mycelium of nematophagous fungi. J. Gen. Microb. 112: 89–93.Google Scholar
  43. 43.
    Nordbring-Hertz, B., 1972. Scanning electron microscopy of the nematode-trapping organs in Anhrobotrys oligospora. Physiol. Plant 26: 279–284.Google Scholar
  44. 44.
    Nordbring-Hertz, B. & C. Brink, 1974. Qualitative characterization of some peptides inducing morphogenesis in the nematode-trapping fungus Athrobotrys oligospora. Physiol. Plant. 31: 59–63.Google Scholar
  45. 45.
    Peters, B. G., 1955. A note on simple methods of recovering nematodes from soil. In D. K. Kevan (ed.), Soil Zoology. Proc. Univ. Nottingham, 2nd. Easter School of Agricultural Science. Butterworths, London, pp. 373–374.Google Scholar
  46. 46.
    Peterson, E. A. & H. Katznelson, 1965. Studies on the relationship between nematode-trapping fungi in the vicinity of plants roots. Can. J. Microbiol. 11: 491–495.Google Scholar
  47. 47.
    Pramer, D. & N. R. Stoll, 1959. Nemin, a morphogenic substance causing trap formation by predaceous fungi. Science 129: 966–967.Google Scholar
  48. 48.
    Schenck, S., W. B. Kendrick & D. Pramer, 1977. A new nematode-trapping hyphomycete and a re-evaluation of Dactylaria and Arthrobotrys. Can. J. Bot. 55: 977–985.Google Scholar
  49. 49.
    Shepherd, A. M., 1955. Some observations on the distribution and biology of fungi predaceous on nematodes. Ph.D. Thesis, University of London.Google Scholar
  50. 50.
    Sohlenius, B., 1979. A carbon budget for nematodes, rotifers and tardigrades in a Swedish coniferous forest soil. Hol. Ecol. 2: 30–40.Google Scholar
  51. 51.
    Tribe, H. T., 1980. Prospects for the biological control of plant-parasitic nematodes. Parasitology 81: 619–639.Google Scholar
  52. 52.
    Whitehead, A. G. & J. R. Hemmings, 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Ann. appl. Biol. 55: 25–38.Google Scholar
  53. 53.
    Wood, F. H., 1973. Nematode-trapping fungi from a tussock grassland soil in New Zealand. N. Z. J. Bot. 11: 231–240.Google Scholar
  54. 54.
    Wyborn, C. H. E., D. Priest & C. L. Duddington, 1969. Selective technique for determination of nematophagous Fungi in soils. Soil Biol. Biochem. 1: 101–102.Google Scholar

Copyright information

© Dr W. Junk Publisher 1984

Authors and Affiliations

  • N. F. Gray
    • 1
  1. 1.Environmental Sciences Unit, Trinity CollegeUniversity of DublinDublin 2Ireland

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