Résumé
La composition chimique des parois d'une levure Candida cultivée sur substrat n paraffine a été examinée. Par rapport à celles de levure du même genre, les parois de cette souche renferment moins d'oses neutres mais davantage de protéines et glucosamine. Une partie importante de cette hexosamine entre dans la constitution de la chitine qui représente 22% de la paroi.
Le traitement par l'éthylène diamine permet de séparer trois fractions A, B et C dont les structures apparaissent plus complexes que celles obtenues à partir des parois de levures Saccharomyces et autres Candida.
La composition chimique originale de ces parois et les complexes glycoprotéiques particuliers obtenus après différents traitements de la paroi peuvent constituer un caractère d'identification de cette souche.
Summary
The cell wall chemical structure of a Candida yeast strain cultivated on a paraffin substrate has been investigated. By comparison with other yeasts of the same generus, the cell walls of this strain contain less neutral sugar but more proteins and glucosamine. An important part of this aminosugar constitutes chitine which represents 22% of the cell wall.
By ethylene diamine treatment three fractions A, B, C are obtained whose structure seems more complex than those obtained from Saccharomyces and other Candida cell walls.
The original chemical composition of these cell walls and the particular glycoprotein complexes obtained after different treatments of the cell walls may be used as an identification character of this strain.
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Morilhat, J.P., Leleu, J.B. & Bonaly, R. Études sur la composition chimique des parois d'une levure candida après croissance sur n paraffine. Mycopathologia 61, 49–54 (1977). https://doi.org/10.1007/BF00440758
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DOI: https://doi.org/10.1007/BF00440758