Summary
Fractionation of proteins of mycelial cell free extracts from Saprolegnia monoica revealed the presence of two different (1.3) β glucanases. The most important fraction exhibited activity against laminarin and p. nitrophenyl BD. glucopyranoside. The other was active on both laminarin and oxidized laminarin. This endo-glucanase represented the main part of glucanase activities released during cell wall autolysis. Properties and cellular distribution of these enzymes are discussed in respect to their morphogenetic role in hyphal differentiation.
Similar content being viewed by others
References
Farkas, V., P. Biely & S. Bauer 1973. Extracellular β glucanases of the yeast Saccharomy-cescerevisiae. Biochimica et Biophysica Acta 321: 246–255.
Fèvre, M. 1972. Contribution to the study of the determination of mycelium branching of Saprolegnia monoica Pringsheim. Zeitschrift für Pflanzen-physiologie 68: 1–10.
Fèvre, M. 1976. Recherches sur le déterminisme de la morphogenèse hyphale. Aspects enzymatiques de la croissance et de la ramification des hyphes de Saprolegnia monoica Pringsheim. Thèse Doctorat d'Etat. Université de LYON.
Fèvre, M. 1977. Subcellular localization of glucanase and cellulase in Saprolegnia monoica Pringsheim. Journal of General Microbiology 103: 287–295.
Fèvre, M. 1979. Glucanase, glucan synthetases and wall growth. In ‘Wall and hyphal growth’ Editors. Burnett J. H. & A.P.J. Trinci. Cambridge University Press (in press).
Fèvre, M., G. Turian & J.P. Larpent 1974. Bourgeonnements et croissance hyphale fongiques. Homologies structurales et fonctionnelles modeles Neurospora et Saprolegnia. Physiologie Végétale 13: 23–38.
Fleet, G.H. & H.J. Phaff 1973. Effect of glucanases of yeast and Bacterial origin on cell walls of Schizosaccharomyces species, p. 33–59. In ‘Yeast mould and Plant Protoplasts’. Academic Press. Editors Villanueva J.R., I. Garcia-Acha, S. Gascon & F. Uruburu.
Fleet, G.H. & H.J. Phaff 1974. Glucanases in Schizosaccharomyces. Isolation and properties of the cell wall associated β (1–3) glucanases. The Journal of Biological chemistry 249: 1717–1728.
Kritzman, G., I. Chet & Y. Henis, 1978. Localization of β 1–3 glucanase in the mycelium of Sclerotium rolfsii. Journal of Bacteriology 134: 470–475.
Machlis, L. 1953. Growth and nutrition of water molds in the subgenus Euallomyces. II Optimal composition of the minimal medium. American Journal of Botany 40: 449–460.
Mahadevan, P.R. & U.R. Mahadkar, 1970. Role of enzymes in growth and morphology of Neurospora crassa: cell wall bound enzymes and their possible role in branching. Journal of Bacteriology 104: 941–947.
Miller, G.L. 1959. Use of dinitrosalycilic acid reagent for determination of reducing sugar. Analytical Chemistry 31: 426–428.
Polacheck Y. & R.F. Rosenberger, 1975. Autolytic enzymes in hyphae of Aspergillus nidulans: their action on old and newly formed walls. Journal of Bacteriology 121: 332–337.
Sukumaran C.P. & P.R. Mahadevan, 1975. Localization of enzymes in the cell wall of Neurospora crassa. Indian Journal of Experimental Biology 13: 127–130.
Thomas D.S.D. & J.T. Mullins, 1969. Cellulase induction and wall extension in the water mold Achlya ambixesualis. Physiologia Plantarum 22: 347–353.
Villa T.G., V. Notario & J.R. Villanueva, 1975. β glucanases of the yeast Pichia polymorpha. Archives of Microbiology 104: 201–206.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fevre, M. Intracellular and cell wall associated (1 → 3) β glucanases of Saprolegnia. Mycopathologia 67, 89–94 (1979). https://doi.org/10.1007/BF00440678
Issue Date:
DOI: https://doi.org/10.1007/BF00440678