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Induction of monocyte chemotaxis in devascularized rabbit bone

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Summary

Temporary impairment of blood supply has been suggested to cause bone remodeling. The degradation of cells and matrix and the attraction of resorbing cells were examined in this study. Bone specimens of rabbits were stored in vitro for 2–20 days. At the end of this aging process the probes were tested for their chemotactic activity toward autologous leukocytes in a diffusion chamber. Both supernatant from the aged bone specimens and ground bone particles exhibited significant chemotactic activity that was specifically attracting monocytes. It is suggested that soluble bone matrix proteins or degeneration products liberated during ischemic damage to cortical bone initiate the resorptive process.

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References

  1. Boyden S (1962) The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med 115:453–466

    Google Scholar 

  2. Böyum A (1968) Separation of leucocytes from blood and bone marrow. Scand J Clin Lab Invest 21 [Suppl 97]:1–109

    Google Scholar 

  3. Falk W, Goodwin RH jr, Leonard EJ (1980) A 48-well micro chemotaxis assembly for rapid and accurate measurement of leucocyte migration. J Immunol Methods 33:239–247

    Google Scholar 

  4. Falk W, Harvath L, Leonard EJ (1982) Functionally distinct subpopulations of human monocytes: receptors for f-Met-Leu-Phe are expressed only on the chemotactically active cells. In: Norman S, Sorkin E (eds) Macrophages and natural killer cells. Plenum, New York, pp 101–106

    Google Scholar 

  5. Gautier E, Cordey J, Mathys R, Rahn BA, Perren SM (1984) Porosity and remodelling of plated bones after internal fixation: results of stress shielding or vascular damage? In: Ducheyne P, van der Perre G, Aubert AE (eds) Biomaterials and biomechanics 1983. Elsevier Science, Amsterdam, pp 195–200

    Google Scholar 

  6. Kahn AJ, Malone JD, Teitelbaum SL (1981) Osteoclast precursors, mononuclear phagocytes, and bone resorption. Trans Assoc Am Physicians 94:267–278

    Google Scholar 

  7. Keller HU, Sorkin E (1967) Studies on chemotaxis. IX. Migration of rabbit leucocytes through filter membranes. Proc Soc Exp Biol Med 126:677–680

    Google Scholar 

  8. Keller H, Wissler JH, Damerau B, Hess MW, Cottier H (1980) The filter technique for measuring leucocyte locomotion in vitro. Comparison of three modifications. J Immunol Methods 36:41–53

    Google Scholar 

  9. Loutit JF, Nisbet NW (1982) The origin of the osteoclast. Immunobiology 161:193–203

    Google Scholar 

  10. Malone JD, Teitelbaum SL, Griffin GL, Senior RM, Kahn AJ (1982) Recruitment of osteoclast precursors by purified bone matrix constituents. J Cell Biol 92:227–230

    Google Scholar 

  11. Marks SC jr, Walker DG (1981) The hematogeneous origin of osteoclasts: experimental evidence from osteopetrotic mice treated with spleen cells from beige mouse donors. Am J Anat 161:1–10

    Google Scholar 

  12. Minkin C, Bannon DJ, Pokress S (1985) Bone-derived macrophage chemotactic factors: methods of extraction and further characterization. Calcif Tissue Int 37:63–77

    Google Scholar 

  13. Mundy GR, Altmann AJ, Gondek MD, Bandelin JG (1977) Direct resorption of bone by human monocytes. Science 196:1109–1111

    Google Scholar 

  14. Mundy GR, Varani J, Orr W, Gondek MD, Ward PA (1978) Resorbing bone is chemotactic for monocytes. Nature 275:132–135

    Google Scholar 

  15. Mundy GR (1982) Role of monocytes in bone resorption. Adv Exp Med Biol 151:401–408

    Google Scholar 

  16. Mundy GR, Poser JW (1983) Chemotactic activity of the gamma-carboxyglutamic acid-containing protein in bone. Calcif Tissue Int 35:164–168

    Google Scholar 

  17. Orr FW, Varani J, Gondek MD, Ward PA, Mundy GR (1980) Partial characterization of a bone-derived chemotactic factor for tumor cells. Am J Pathol 99:43–52

    Google Scholar 

  18. Pertoft H, Johnsson A, Waermegard B, Seljelid R (1980) Separation of human monocytes on density gradients of percoll. J Immunol Methods 33:221–229

    Google Scholar 

  19. Pfister U, Rahn BA, Perren SM, Weller S (1979) Vaskularität und Knochenumbau nach Marknagelung langer Röhrenknochen. Akt Traumatol 9:191–195

    Google Scholar 

  20. Sampath TK, Reddi AH (1981) Dissociative extraction and reconstitution of extracellular matrix components involved in local bone differentiation. Proc Natl Acad Sci USA 78:7599–7603

    Google Scholar 

  21. Sarkar MR, Rahn BA, Poster U, Keller HU, Perren SM (in preparation) Postoperative changes of leucocyte responsiveness to chemotactic stimuli in rabbits

  22. Schiffmann E, Corcoran BA, Wahl SN (1975) N-formylmethionyl peptides as chemoattractants for leucocytes. Proc Natl Acad Sci USA 72:1059–1062

    Google Scholar 

  23. Seifert MF (1984) The biology of macrophages in osteopetrosis. Clin Orthop 182:270–277

    Google Scholar 

  24. Sminia T, Dijkstra CD (1986) The origin of osteoclasts: an immunohistochemical study on macrophages and osteoclasts in embryonic rat bone. Calcif Tissue Int 39:263–266

    Google Scholar 

  25. Snyderman R, Pike MC (1984) Chemoattractant receptors on phagocytic cells. Annu Rev Immunol 7:257–281

    Google Scholar 

  26. Somerman M, Hewitt AT, Warner HH, Schiffmann E, Termine J, Reddi AH (1983) Identification of a bone matrix-derived chemotactic factor. Calcif Tissue Int 35:481–485

    Google Scholar 

  27. Tinkler SMB, Williams DM, Linder JE, Johnson NW (1983) Kinetics of osteoclast formation: the significance of blood monocytes as osteoclast precursors during 1α-hydroxy-cholecalciferol-stimulated bone resorption in the mouse. J Anat 137:335–340

    Google Scholar 

  28. Uhthoff HK, Dubuc FL (1971) Bone structure changes in the dog under rigid internal fixation. Clin Orthop 81:165–170

    Google Scholar 

  29. Uhthoff HK, Finnegan MA (1983) The effects of metal plates on posttraumatic remodelling and bone mass. J Bone Joint Surg [Br] 65:66–71

    Google Scholar 

  30. Young RW (1962) Cell proliferation and specialization during endochondral osteogenesis in young rats. J Cell Biol 14:357–370

    Google Scholar 

  31. Zallone AZ, Teti A, Primavera MV, Naldini L, Marchisio RC (1983) Osteoclasts and monocytes have similar cytoskeletal structures and adhesion property in vitro. J Anat 237:57–60

    Google Scholar 

  32. Zallone AT, Teti A, Primavera MV (1983) Studio microcinematografico di fusione in vitro tra monociti ematici ed osteoclasti differenziati. Boll Soc Ital Biol Sper 59:1959–1965

    Google Scholar 

  33. Zigmond SH, Hirsch JG (1973) Leucocyte locomotion and chemotaxis. New methods for evaluation, and demonstration of a cell-derived chemotactic factor. J Exp Med 137:387–410

    Google Scholar 

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Authors and Affiliations

  1. Abteilung für Unfallchirurgie, Städtisches Klinikum, Karlsruhe, Germany

    M. R. Sarkar & U. Pfister

  2. Labor für experimentelle Chirurgie, Schweizerisches Forschungsinstitut, Davos, Switzerland

    B. A. Rahn & S. M. Perren

  3. Institut für Pathologie, Universität Bern, Bern, Switzerland

    H. U. Keller

Authors
  1. M. R. Sarkar
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  2. B. A. Rahn
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  3. U. Pfister
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  4. H. U. Keller
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  5. S. M. Perren
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Additional information

This work was supported by the Swiss National Science Foundation, grant no. 3.857.0.83, and by the Studienstiftung des deutschen Volkes

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Sarkar, M.R., Rahn, B.A., Pfister, U. et al. Induction of monocyte chemotaxis in devascularized rabbit bone. Arch Orthop Trauma Surg 109, 97–101 (1990). https://doi.org/10.1007/BF00439387

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