Abstract
A guinea pig model was used to evaluate immune response to Cryptococcus neoformans. This model shared characteristics with cryptococcosis in humans. Twenty five guinea pigs injected intraperitoneally with 107 viable C. neoformans cells developed diseminated disease. Forty days after infection all guinea pigs were killed and autopsy performed. C. neoformans growth in the lungs, brains, livers and spleen of the infected animals were determined. Furthermore, the immune response was characterized by moderate degree of delayed-type hypersensitivity and humoral response.
In some organs was observed neutrophyle and lymphocyte infiltration with presence of cryptococci cells. The infiltration observed in the organs was probably a consequence of an immune reaction.
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Lewis, J. L. & Ravinovich, S., 1972. The wide spectrum of cryptococcal infections. Am. J. Med. 53: 315–322.
Spickard, A., Butler, W., Andriole, V. & Utz, J., 1963. The improved prognosis of cryptococcal Meningitis with Amphotericin B therapy. Annals of Internal Medicine 58: 66–83.
Graybill, R. D. & Alford, R. H., 1974. Cell mediated immunity in Cryptococcosis. Cell Immunol. 14: 12–21.
Diamond, R. D., Root, R. K. & Bennet, J. E., 1972. Factors influencing killing of Cryptococcus neoformans by human leukocytes ‘in vitro’. J. of Infections Diseases 125: 367–376.
Kozel, T. R., 1977. Non-encapsulated variant of Cryptococcus neoformans II. Surface receptors for cryptococcal polysaccharide and their role in inhibition of phagocytosis by polysaccharide. Infect. Immun. 16: 99–106.
Kozel, T. R. & Cazin, J., Jr., 1971. Non-encapsulated variant of Cryptococcus neoformans. I. Virulence studies and characterization of soluble polysaccharide. Infect. Immun. 3: 287–294.
Kozel, T. R. & Cazin, J., Jr., 1974. Induction of humoral antibody response by soluble polysaccharide of Cryptococcus neoformans. Mycopathologia 54: 21–30.
Diamond, R. D., May, J. E., Kane, M. A., Frank, M. M. & Bennett, J. E., 1974. The role of the classical and alternate complement pathways in host defenses against Cryptococcus neoformans infection. J. of Immunology 112: 2260–2270.
Kozel, T. R. & McCaw, T. G., 1979. Opsonization of Cryptococcus neoformans by human immunoglobulin G: Role of immunoglobulin G in phagocytosis by macrophages. Infect. Immun. 25: 255–261.
Bindschadler, D. D. & Bennett, J. E., 1968. Serology of human cryptococcosis. Annals of Internal Medicine 69: 45–52.
Diamond, R. D. & Bennet, J. E., 1974. Prognostic factors in cryptococcal Meningitis. A study of 111 cases. Annals of Internal Medicine 80: 176–181.
Graybill, J. R., Mickey, H. & Drutz, D. J., 1981. Passive immunization in murine cryptococcosis. Sabouraudia. 19: 237–244.
Cauley, L. K. & Murphy, J. W., 1979. Response of congenitally athymic (nude) and phenotypically normal mice to a Cryptococcus neoformans infection. Infect. Immun. 23: 644–651.
Diamond, R. D., 1977. Effects of stimulation and suppression of cell-mediated immunity on experimental cryptococcosis. Infect. Immun. 17: 187–194.
Riera, C. M., Masih, D. T. & Nobile, R., 1981. Inmunidad en criptococcosis experimental. X Jornadas Argentinas de Micología. S. M. de Tucumán. Argentina.
Hay, R. J. & Reiss, E., 1978. Delayed-type hypersensitivity responses in infected mice elicited by cytoplasmic fractions of Cryptococcus neoformans. Infect. Immun. 22: 72–79.
Lim, T. S., Murphy, J. W. & Cauley, L. K., 1980. Host-etiological agent interactions in intranasally and intraperitoneally induced cryptococcosis in mice. Infect. Immun. 29: 633–641.
Negroni, R. & Iovanitti de Flores, C., 1971. La inmunofluorescencia indirecta en el diagnóstico serológico de las candidiasis sistémicas. Mycopathologia 43: 355–359.
Yantorno, C., Debanne, M. & Vottero-Cima, E., 1971. Autoimmune orchitis induced by autoimmunization with seminal plasma in the rabbit. J. Reprod. Fertil. 27: 311–320.
Riera, C. M., Galmarini, M., Faillaci, M. & Yantorno, C., 1980. Detection of autoimmune response to rabbit epididymal seminal spermatozoa.
Boyden, S., 1951. The absortion of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiproteins sera. J. Exp. Med. 93: 107–120.
Ouchterlony, O., 1958. Diffusion in gel methods for immunological analysis. Progress in Allergy 51: 1–78.
Drouhet, E., 1973. Electrosynerese (Immunoelectrodiffusion) en agarose, methode rapide et sensible pour le diagnostic des candidoses septicemigues et visceral. Resultats comparatifs avec d'autres techniques d'immunodiffusion et d'immunofluorescence. Bulletin de la Société Française de Mycologie Medicale 2: 11–14.
Dykstra, M. A. & Friedman, L., 1978. Pathogenesis, lethality, and immunizing effect of experimental cutaneous cryptococcosis. Infect. Immun. 20: 446–455.
Karaoui, R. M., Hall, N. K. & Larsh, H. W., 1977. Role of macrophages in immunity and pathogenesis of experimental cryptococcosis induced by the airborne route. I. Pathogenesis and acquired immunity of Cryptococcus neoformans. Mykosen. 20: 380–388.
Masih, D. T., 1981. Criptococosis experimental en cobayos. Tesis de Doctorado. Universidad Nacional de Córdoba.
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Riera, C.M., Masih, D.T. & Nobile, R. Experimental cryptococcosis in guinea pigs. Mycopathologia 82, 179–184 (1983). https://doi.org/10.1007/BF00439224
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DOI: https://doi.org/10.1007/BF00439224