, Volume 82, Issue 3, pp 175–178 | Cite as

Effect of formalin on the survival of systemic fungi in tissue

  • A. Fahmy
  • D. J. Flournoy
  • C. V. Stewart


Stock cultures of Blastomyces dermatitidis, Candida albicans, Cryptococcus neoformans, Histoplasma capsulatum and Sporothrix schenckii were grown in yeast phase on Sabouraud dextrose agar. Standardized suspensions (108 colony forming units/ ml) were made and 1. the organisms treated for various times with 10% formalin/acetate buffer; 2. aliquots injected into the tail veins of Swiss-Webster mice. Minimal fungistatic and fungicidal concentrations of formalin were also determined. Five minutes or more of formalin treatment killed all organisms, in vitro, except C albicans which withstood the 5-minute but not the 30-minute treatment. All fungi, except H. capsulatum which was not recovered, were cultured from mouse heart, kidneys, spleen, lungs and liver prior to but not after 5-minute formalin treatment. All yeasts were susceptible to concentrations of less than 1% formalin. These results emphasize the extreme fungicidal activity of 10% formalin and the uselessness of culturing even very briefly-fixed tissue.


Agar Formalin Candida Dextrose Candida Albicans 
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Copyright information

© Dr W. Junk Publishers 1983

Authors and Affiliations

  • A. Fahmy
    • 1
    • 2
  • D. J. Flournoy
    • 1
    • 2
  • C. V. Stewart
    • 3
  1. 1.Laboratory ServiceVeterans Administration Medical Center (113)Oklahoma CityUSA
  2. 2.Department of PathologyUniversity of Oklahoma Health Sciences CenterOklahoma City
  3. 3.Infectious Disease SectionVeterans Administration Medical CenterOklahoma City

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