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Mycopathologia

, Volume 82, Issue 3, pp 175–178 | Cite as

Effect of formalin on the survival of systemic fungi in tissue

  • A. Fahmy
  • D. J. Flournoy
  • C. V. Stewart
Article

Abstract

Stock cultures of Blastomyces dermatitidis, Candida albicans, Cryptococcus neoformans, Histoplasma capsulatum and Sporothrix schenckii were grown in yeast phase on Sabouraud dextrose agar. Standardized suspensions (108 colony forming units/ ml) were made and 1. the organisms treated for various times with 10% formalin/acetate buffer; 2. aliquots injected into the tail veins of Swiss-Webster mice. Minimal fungistatic and fungicidal concentrations of formalin were also determined. Five minutes or more of formalin treatment killed all organisms, in vitro, except C albicans which withstood the 5-minute but not the 30-minute treatment. All fungi, except H. capsulatum which was not recovered, were cultured from mouse heart, kidneys, spleen, lungs and liver prior to but not after 5-minute formalin treatment. All yeasts were susceptible to concentrations of less than 1% formalin. These results emphasize the extreme fungicidal activity of 10% formalin and the uselessness of culturing even very briefly-fixed tissue.

Keywords

Agar Formalin Candida Dextrose Candida Albicans 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Dr W. Junk Publishers 1983

Authors and Affiliations

  • A. Fahmy
    • 1
    • 2
  • D. J. Flournoy
    • 1
    • 2
  • C. V. Stewart
    • 3
  1. 1.Laboratory ServiceVeterans Administration Medical Center (113)Oklahoma CityUSA
  2. 2.Department of PathologyUniversity of Oklahoma Health Sciences CenterOklahoma City
  3. 3.Infectious Disease SectionVeterans Administration Medical CenterOklahoma City

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