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Candida albicans stress mannoproteins expression in superficial and systemic candidiasis

Stress mannoproteins in Candida albicans

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Abstract

The presence of heat shock mannoproteins (HSMPs) reactive with sIgA was demonstrated in several C. albicans strains. The subculture of the C. albicans isolated from mucosal surfaces on Sabouraud's dextrose agar at 25 °C switched off the HSMP expression. A re-expression of the HSMPs was obtained in the same medium by shifting the temperature of incubation to 37 °C. However, expression of HSMPs in two strains isolated from deep infections was maintained during several subcultures on Sabouraud's dextrose agar at 25 °C. A glycoprotein of 200 kDa seemed to be the main HSMP reacting with vaginal sIgA. The data presented in this study suggest that factors other than temperature can influence the expression of C. albicans HSMPs and therefore these antigens should be referred as stress mannoproteins.

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Abbreviations

HSMPs:

heat shock mannoproteins

MAb:

monoclonal antibody

sIgA:

secretory IgA

References

  1. Odds FC Candida and candidosis. London: Bailliere Tindall, 1988: 1–6.

    Google Scholar 

  2. Vudhichamnong K, Walker DM, Ryley HC. The effect of secretory immunoglobulin A on the in vitro adherence of the yeast Candida albicans to human oral epithelial cells. Arch Oral Biol 1982; 27: 617–621.

    Article  CAS  PubMed  Google Scholar 

  3. Polonelli L, Conti S, Menozzi MG, Gerloni M, Elosegui R, Fernandez M, Alvarez M, Pontón J. Diagnostic potential of IgA coated Candida cells in mucous membrane candidiasis. Mycopathologia 1991; 116: 105–112.

    Article  CAS  PubMed  Google Scholar 

  4. Polonelli L, Gerloni M, Conti S, Fisicaro P, Cantelli C, Portincasa P, Almondo F, Barea PL, Hernando FL, Pontón J. Heat shock mannoproteins as targets of secretory IgA in Candida albicans. J Infect Dis 1994; 169: 1401–1405.

    CAS  PubMed  Google Scholar 

  5. Lupashin VV, Kononova SV, Ratner YN, Tsiomenko AB, Kulaev IS. Identification of a novel secreted glycoprotein of the yeast Saccharomyces cerevisiae stimulated by heat shock. Yeast 1992; 8: 157–169.

    Article  CAS  PubMed  Google Scholar 

  6. Jeavons L, Hunt L, Hamilton A. Immunochemical studies of heat-shock protein 80 of Histoplasma capsulatum. J Med Vet Mycol 1994; 32: 47–57.

    Article  CAS  PubMed  Google Scholar 

  7. Dabrowa N, Howard DH. Heat shock and heat stroke proteins observed during germination of the blastoconidia of Candida albicans. Infect Immun 1984; 44: 537–539.

    CAS  PubMed  Google Scholar 

  8. Dabrowa N, Zeuthen ML, Howard DH. Nutritional stress proteins in Candida albicans. J Gen Microbiol 1990; 136: 1387–1391.

    CAS  PubMed  Google Scholar 

  9. Matthews R, Burnie J. Cloning of a DNA sequence encoding a major fragment of the 47 kilodalton stress protein homologue of Candida albicans. FEMS Microbiology Letts 1989; 60: 25–30.

    CAS  Google Scholar 

  10. Swoboda R, Miyasaki S, Greenspan D, Greenspan JS. Heat-inducible ATP-binding proteins of Candida albicans are recognized by sera of infected patients. J Gen Microbiol 1993; 139: 2995–3003.

    CAS  PubMed  Google Scholar 

  11. Zeuthen ML, Howard DH. Thermotolerance and the heat shock response in Candida albicans. J Gen Microbiol 1989; 135: 2509–2518.

    CAS  PubMed  Google Scholar 

  12. Zeuthen ML, Dabrowa N, Aniebo CM, Howard DH. Ethanol tolerance and the induction of stress proteins by ethanol in Candida albicans. J Gen Microbiol 1988; 134: 1375–1384.

    CAS  PubMed  Google Scholar 

  13. Matthews R, Burnie J, Tabaqchali S. Isolation of immunodominant antigens from sera of patients with systemic candidiasis and characterization of serological response to Candida albicans. J Clin Microbiol 1987; 25: 230–237.

    CAS  PubMed  Google Scholar 

  14. Schneider J, Vicandi J, Regulez P, Quindós G, Pontón J, Cisterna R. Different antibody response against Candida albicans cell wall antigens in cervicovaginal secretions of patients with vulvovaginal candidiasis. Gynecol Obstet Invest 1990; 30: 174–177.

    Article  CAS  PubMed  Google Scholar 

  15. Pontón J, Marot-Leblond A, Ezkurra PA, Barturen B, Robert R, Senet JM. Characterization of Candida albicans cell wall antigens with monoclonal antibodies. Infect Immun 1993; 61: 4842–4847.

    PubMed  Google Scholar 

  16. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 1970; 227: 680–685.

    Article  CAS  Google Scholar 

  17. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350–4354.

    Article  CAS  PubMed  Google Scholar 

  18. Lehner T. Immunofluorescence study of Candida albicans in candidiasis, carriers and controls. J Pathol Bacteriol 1966; 91: 97–104.

    Article  CAS  PubMed  Google Scholar 

  19. Waldman RH, Cruz JM, Rowe DS. Immunoglobulin levels and antibody to Candida albicans in human cervicovaginal secretions. Clin exp Immunol 1972; 10: 427–434.

    CAS  PubMed  Google Scholar 

  20. Gough PM, Warnock DW, Richardson MD, N.J M, King JM. IgA and IgG antibodies to Candida albicans in the genital tract secretions of women with or without vaginal candidosis. Sabouraudia: J Med Vet Mycol 1984; 22: 265–271.

    Article  CAS  Google Scholar 

  21. Romero-Piffiguer MD, Vucovich PR, Riera CM. Secretory IgA and secretory component in women affected by recidivant vaginal candidiasis. Mycopathologia 1985; 91: 165–170.

    Article  CAS  PubMed  Google Scholar 

  22. Poulain D, Vernes A, Fruit J. Variation de la structure antigénique pariètale de Candida albicans. Mise en évidence au niveau des blastospores d'un antigéne P dépendent de leur origine. Sabouraudia 1980; 18: 61–68.

    Google Scholar 

  23. Hernando FL, Estevez JJ, Cebrian M, Poulain D, Pontón J. Identification of Candida albicans cell wall antigens lost during subculture in synthetic media. J Med Vet Mycol 1993; 31: 227–237.

    Article  CAS  PubMed  Google Scholar 

  24. Poulain D, Tronchin G, Lefevbre B, Husson MO. Antigenic variability between Candida albicans blastospores isolated from healthy subjects and patients with Candida infection. Sabouraudia 1982; 20: 173–177.

    CAS  PubMed  Google Scholar 

  25. Brawner DL, Cutler JE. Variability in expression of cell surface determinant on Candida albicans as evidenced by an agglutinating monoclonal antibody. Infect Immun 1984; 43: 966–972.

    CAS  PubMed  Google Scholar 

  26. Brawner DL, Cutler JE. Ultrastructural and biochemical studies of two dynamically expressed cell surface determinants on Candida albicans. Infect Immun 1986; 51: 327–336.

    CAS  PubMed  Google Scholar 

  27. Brawner DL, Cutler JE. Cell surface and intracellular expression of two Candida albicans antigens during in vitro and in vivo growth. Microb Pathogen 1987; 2: 249–257.

    Article  CAS  Google Scholar 

  28. Poulain D, Vernes A, Biguet J. Variation of the antigenic structure of Candida albicans blastospores cell wall. Practical repercussions. In: Preuser HJ (ed), Medical Mycology. Stuttgart: Gustav Fisher Verlag, 1980, 147–156.

    Google Scholar 

  29. Vidotto V, Vieta I, Fisicaro P, Conti S, Gerloni M, Cantelli C, Ponton J, Polonelli. L. Influence of glucose and ammonium on the reactivity in vitro of Candida albicans heat shock mannoproteins with secretory IgA. J Mycol Méd 1994; 4: 201–204.

    Google Scholar 

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Authors and Affiliations

  1. Departamento de Inmunologia, Microbiologia y Parasitología, Facultad de Medicina y Odontologia, Universidad del Pais Vasco, Apartado 699, E-48080, Bilbao, Spain

    Jose Pontón

  2. Facultad de Ciencias, Universidad del Pais Vasco, Apartado 699, E-48080, Bilbao, Spain

    Fernando L. Hernando & Pedro L. Barea

  3. Departamento de Enfermería I, Universidad del Pais Vasco, Apartado 699, E-48080, Bilbao, Spain

    Maria Dolores Moragues

  4. Istituto di Microbiologia, Università degliStudi di Parma, Viole A. Gramsci 14, I-43100, Parma, Italy

    Mara Gerloni, Stefania Conti, Paola Fisicaro, Cristina Cantelli & Luciano Polonelli

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  1. Jose Pontón
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  2. Fernando L. Hernando
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  3. Maria Dolores Moragues
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  4. Pedro L. Barea
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  5. Mara Gerloni
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  6. Stefania Conti
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  7. Paola Fisicaro
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  8. Cristina Cantelli
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  9. Luciano Polonelli
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Pontón, J., Hernando, F.L., Moragues, M.D. et al. Candida albicans stress mannoproteins expression in superficial and systemic candidiasis. Mycopathologia 133, 89–94 (1996). https://doi.org/10.1007/BF00439119

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  • DOI: https://doi.org/10.1007/BF00439119

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