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Molecular and General Genetics MGG

, Volume 110, Issue 4, pp 299–306 | Cite as

Induction of prophage λ in a mutant of E. coli K12 defective in initiation of DNA replication at high temperature

  • Marilyn Monk
  • Julian Gross
Article

Summary

Prophage λ is not induced when DNA synthesis ceases at 42°C in a mutant of E. coli which is unable to initiate rounds of DNA replication at high temperature. However, induction occurs when the cells are UV irradiated after completion of rounds of replication at 42°C. Evidence is presented that the uvr functions, necessary for dimer excision, are not required for this induction, and that the UV irradiation itself does not provoke net host DNA synthesis under these conditions. We conclude that prophage induction can result from irradiation damage in chromosomes that are unable to replicate.

Keywords

Irradiation Damage Prophage Induction Dime Excision 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Anderson, E. H.: Growth requirements of virus-resistant mutants of Escherichia coli strain “B”. Proc. nat. Acad. Sci. (Wash.) 32, 120 (1946).Google Scholar
  2. Appleyard, R. K.: Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia coli K12. Genetics 39, 440 (1954).Google Scholar
  3. Borek, E., Ryan, A.: The transfer of a biologically active irradiation product from cell to cell. Biochim. biophys. Acta (Amst.) 41, 67 (1960).Google Scholar
  4. Boyce, R. P., Howard-Flanders, P.: Release of ultraviolet light-induced thymine dimers from DNA in E. coli K12. Proc. nat. Acad. Sci. (Wash.) 51, 293 (1964).Google Scholar
  5. Brooks, K., Clark, A. J.: Behaviour of λ bacteriophage in a recombination deficient strain of Escherichia coli. J. Virol. 1, 283 (1967).Google Scholar
  6. Byfield, J. E., Scherbaum, O. H.: A rapid radioassay technique for cellular suspensions. Analyt. Biochem. 17, 434 (1966).Google Scholar
  7. Devoret, R., George, J.: Induction indirecte du prophage λ par le rayonnement ultraviolet. Mutation Res. 4, 713 (1967).Google Scholar
  8. —: Indirect induction of prophage λ and colicin I factor. Zbl. Bakt., I. Abt. Orig. 196, 193 (1965).Google Scholar
  9. Freifelder, D.: Studies with Escherichia coli sex factors. Cold Spr. Harb. Symp. quant. Biol. 33, 425 (1968).Google Scholar
  10. Hirota, Y., Ryter, A., Jacob, F.: Thermosensitive mutants of E. coli affected in the process of DNA synthesis and cellular division. Cold spr. Harb. Symp. quant. Biol. 33, 317 (1968).Google Scholar
  11. Kohiyama, M.: DNA synthesis in temperature sensitive mutants of Escherichia coli. Cold Spr. Harb. Symp. quant. Biol. 33, 317 (1968).Google Scholar
  12. Latarjet, R., Morenne, P., Berger, R.: Un appareil simple pour le dosage des rayonnements ultraviolets émis par les lampes germicides. Ann. Inst. Pasteru 85, 174 (1953).Google Scholar
  13. Mattern, I. E., Winden, M. P. van, Rörsch, A.: The range of action of genes controlling radiation sensitivity in Escherichia coli. Mutation Res. 2, 111 (1965).Google Scholar
  14. Monk, M.: Induction of phage λ by transferred irradiated colI DNA. Molec. Gen. Genetics 106, 14 (1969).Google Scholar
  15. Monk, M., Peacey, M., Gross, J. D.: Repair of damage induced by ultraviolet light in DNA polymerase-defective E. coli cells. In press (1971).Google Scholar
  16. Nishimura, Y., Caro, L., Berg, C. M., Hirota, Y.: Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome. J. molec. Biol. in press (1971).Google Scholar
  17. Okada, T., Yanagisawa, K., Ryan, F. J.: Elective production of thymineless mutants. Nature (Lond.) 188, 340 (1960).Google Scholar
  18. Radman, M., Cordone, L., Kismanovic-Simic, D., Errera, M.: Complementary action of recombination and excision in the repair of ultraviolet irradiation damage to DNA. J. molec. Biol. 49, 203 (1970).Google Scholar
  19. Rosner, J. L., Kass, L. R., Yarmolinsky, M. B.: Parallel behaviour of F and P1 in causing indirect induction of lysogenic bacteria. Cold Spr. Harb. Symp. quant. Biol. 33, 785 (1968).Google Scholar
  20. Rupp, D., Howard-Flanders, P.: Discontinuities in the DNA synthesised in an excisiondefective strain of Escherichia coli following ultraviolet irradiation. J. molec. Biol. 31, 291 (1968).Google Scholar
  21. Scaife, J., Gross, J. D.: Inhibition of multiplication of an F-lac factor in Hfr cells of Escherichia coli K12. Biochem. biophys. Res. Commun. 7, 403 (1962).Google Scholar
  22. Sueoka, N., Yoshikawa, H.: The chromosome of Bacillus subtilis. 1. Theory of marker frequency analysis. Genetics 52, 747 (1965).Google Scholar
  23. Taylor, A. L.: Current linkage map of Escherichia coli. Bact. Rev. 34, 155 (1970).Google Scholar

Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • Marilyn Monk
    • 1
  • Julian Gross
    • 1
  1. 1.MRC Molecular Genetics Unit, Department of Molecular BiologyUniversity of EdinburghEdinburghUK

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