Molecular and General Genetics MGG

, Volume 110, Issue 4, pp 299–306 | Cite as

Induction of prophage λ in a mutant of E. coli K12 defective in initiation of DNA replication at high temperature

  • Marilyn Monk
  • Julian Gross


Prophage λ is not induced when DNA synthesis ceases at 42°C in a mutant of E. coli which is unable to initiate rounds of DNA replication at high temperature. However, induction occurs when the cells are UV irradiated after completion of rounds of replication at 42°C. Evidence is presented that the uvr functions, necessary for dimer excision, are not required for this induction, and that the UV irradiation itself does not provoke net host DNA synthesis under these conditions. We conclude that prophage induction can result from irradiation damage in chromosomes that are unable to replicate.


Irradiation Damage Prophage Induction Dime Excision 
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Copyright information

© Springer-Verlag 1971

Authors and Affiliations

  • Marilyn Monk
    • 1
  • Julian Gross
    • 1
  1. 1.MRC Molecular Genetics Unit, Department of Molecular BiologyUniversity of EdinburghEdinburghUK

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