Cell Biology and Toxicology

, Volume 12, Issue 4–6, pp 341–344 | Cite as

Description of an in vitro angiogenesis model designed to test antiangiogenic molecules

  • B. Vailhé
  • X. Ronot
  • M. Lecomte
  • N. Wiernsperger
  • L. Tranqui
Selected posters

Abstract

Angiogenesis is involved in numerous pathologies. Studies with in vitro models allow the description and analysis of the different steps involved in this process under defined culture conditions. We describe a controllable and reproducible in vitro model. We assessed the usefulness of this model with two different cell lines: human umbilical vein endothelial cells and bovine retinal endothelial cells. These cells reorganize themselves and change their phenotypes within 24 h after seeding under our culture conditions (low human serum percentage, defined cell density, fibrin matrix) to form ‘capillary-like structures’ (CLS) in vitro. We showed that, depending on the cell line used, the fibrinolytic activity of the cells was a determining factor which could induce or prevent the formation of the CLS. Inhibitors of angiogenesis can be tested using such a model.

Key words

endothelial cells fibrin in vitro angiogenesis 

Abbreviations

HUVEC

human umbilical vein endothelial cells

BREC

bovine retinal endothelial cells

CLS

capillary-like structure

HS

human serum

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • B. Vailhé
    • 1
  • X. Ronot
    • 2
    • 3
  • M. Lecomte
    • 4
  • N. Wiernsperger
    • 4
  • L. Tranqui
    • 1
  1. 1.Laboratoire de Bioénergétique Fondamentale et AppliquéeUniversité Joseph FourierGrenoble Cédex
  2. 2.Laboratoire Dynamique de l'Organisation des Génomes, Institut AlbertBonniotUniversité Joseph FourierLa Tronche Cédex
  3. 3.Laboratoire de Neurobiologie du DéveloppementE.P.H.E.Montpellier Cédex
  4. 4.Lipha-INSERM U352INSA LyonVilleurbanneFrance

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