Detection and determination of benzophenone present in diphenylhydantoin sodium formulations and drug substance

Nachweis und Bestimmung von Benzophenon in Natriumdiphenylhydantoin-Formulierungen und -Reinsubstanz

Zusammenfassung

Der Nachweis erfolgt dünnschicht-chromatographisch auf Silicagel 60 F mit Toluol/Methanol/Äthylacetat/Essigsäure/Chloroform (80∶20∶15∶10∶5) oder Äthylacetat/Methanol/Ammoniak (85∶10∶5) als Lösungsmittel und durch Sichtbarmachung mit UV-Licht. Die Bestimmung wird durch Absorptionsmessung bei 248 nm vorgenommen. Das Verfahren wird zur Produktionskontrolle empfohlen.

Summary

The detection of benzophenone in sodium diphenylhydantoin formulations and drug substance is carried out by thin-layer chromatography on silica gel 60 F using toluene/methanol/ethyl acetate/acetic acid/chloroform (80∶20∶15∶10∶5) or ethyl acetate/methanol/ammonium hydroxide (85∶10∶5) as solvent and UV-light for visualizing. The determination is performed by measuring the absorption at 248 nm. The method is recommended for production monitoring.

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Matsui, F.F., Smith, S.J. Detection and determination of benzophenone present in diphenylhydantoin sodium formulations and drug substance. Z. Anal. Chem. 275, 365–367 (1975). https://doi.org/10.1007/BF00437769

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  • Best. von Benzophenon in Diphenylhydantoin
  • Chromatographie, Dünnschicht/Spektralphotometrie, UV