Substrate depletion during formation of aflatoxin and kojic acid on corn inoculated with Aspergillus flavus
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Depletion of sugar and starch carbon sources and concomitant formation of secondary fungal metabolites, aflatoxin and kojic acid, were examined in growing corn inoculated with Aspergillus flavus. Kernels from control and inoculated ears were removed and analyzed after 16, 24, 48, 96 and 168 hrs.
Reducing sugars were not significantly different for inoculated and control non-inoculated samples, but after 168 hrs (seven days) starch content was 20% lower in inoculated than in control samples. Kojic acid was detected before aflatoxins formed. Kojic acid, the oxidized product of kojic acid, and aflatoxin were all present in samples two days from inoculation. The formation of this oxidation product may influence toxin levels.
KeywordsAspergillus flavus starch reducing sugars kojic acid aflatoxin
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- 1.Cuculu AF, Lee LS, Pons WA Jr: Relationship of physical appearance of individual cottonseed to aflatoxin content. J Am Oil Chem Soc 54:235A-237A, 1977.Google Scholar
- 2.Dickens JW, Whitaker TB: Sampling, BGYF, and aflatoxin analysis in corn. Southern Cooperative Series Bulletin 279:35–37, 1983.Google Scholar
- 3.Fleming SE, Reichert RD: Note on a modified method for the quantitative determination of starch. Cereal Chem 57:153–154, 1980.Google Scholar
- 4.Lee LS, Conkerton EJ, Ehrlich KC, Ciegler A: Reducing sugars and minerals from lint of unopened cotton bolls as a substrate for aflatoxin and kojic acid synthesis by Aspergillus flavus. Phytopathology 73:734–736, 1983.Google Scholar
- 5.Lee LS, Lillehoj EB, Kwolek WF: Aflatoxin distribution in individual corn kernels from intact ears. Cereal Chem 57:340–342, 1980.Google Scholar
- 6.Marsh PB, Simpson ME, Ferretti RJ, Merola GV, Donoso J, Craig GO, Trucksess MW, Work PS: Mechanism of formation of a fluorescence in cotton fiber associated with aflatoxins in seeds at harvest. J Agric Food Chem 17:468–472, 1969.Google Scholar
- 7.Malik VS: Genetics and biochemistry of secondary fungal metabolites. Advances in Appl Microbiol 28:28–113, 1982.Google Scholar
- 8.Miller GL, Slater R, Biozgalis R, Blum R: Application of different colorimetric tests to cellodextrins. Anal Biochem 2:521–528, 1961.Google Scholar
- 9.Shotwell OL, Goulden ML, Hesseltine CW: Aflatoxin: Distribution in contaminated corn. Cereal Chem 51:492–496, 1974.Google Scholar