Mycopathologia

, Volume 94, Issue 2, pp 75–78 | Cite as

The kinetics of the autolytic phase of growth in cultures of Aspergillus niger

  • R. Lahoz
  • F. Reyes
  • G. Gómez Alarcón
  • L. Cribeiro
  • M. A. Junquera
  • R. Lahoz-Beltrá
Article

Abstract

The kinetics of the autolytic phase of growth in cultures of Aspergillus niger has been studied. Two different autolytic periods could be distinguished. One, consisting of a rapid (exponential) loss (62%) of mycelial weight, occurred between 36 and 117 hours of incubation. A second, consisting of a slow autolysis, occurred between the 117th and the 190th hour of incubation; the mycelial loss here being 5%. Based on the degree of autolysis (α=67.0%), 92.5% and 7.5% are lost during the first and the second autolytic periods, respectively.

Keywords

Autolysis 

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References

  1. 1.
    Behr G: Über Autolyse bei Aspergillus niger. Arch Mikrobiol 1:418–444, 1930.Google Scholar
  2. 2.
    Emiliani E, Retamar JA: Estudio sobre la inductión de la autolysis en una cepa de Aspergillus niger. Revista Facultad Ingehieria Química de la Universidad Nacional del Litoral, Santa Fé, Argentina 26:15–35, 1957.Google Scholar
  3. 3.
    Foster JW: Chemical Activities of Fungi. Academic Press, New York, 1949.Google Scholar
  4. 4.
    Galbraith JC, Smith JE: Filamentous growth of Aspergillus niger in submerged shake culture. Trans Brit Mycol Soc 52:237–246, 1960.Google Scholar
  5. 5.
    Gooday GW, Trinci AP: Wall Structure and Biosynthesis in Fungi. In: GW Gooday & APJ Trinci (Eds), btThe Eukariotic Microbial Cell, pp. 207–251. Symposium 30. The Society for General Microbiology, Cambridge University Press, 1980.Google Scholar
  6. 6.
    Lahoz R, Reyes F, Beltrá R: Some chemical changes in the mycelium of Aspergillus flavus during autolysis. J Gen Microbiol 45:41–49, 1966.Google Scholar
  7. 7.
    Lahoz R, Reyes F, Beltrá R, Gonzalez Ibeas J, Ballesteros AM, Miralles M: Chemical and physiological changes in fungi during autolysis. XI. The exponential law in the induced autolysis of Aspergillus terreus. Microbiología Espaňola 23:253–255, 1970.Google Scholar
  8. 8.
    Lahoz R, Reyes F, Gómez P, Jimeno L: Influence of the carbon/nitrogen relationship on the degree of autolysis of Aspergillus niger. Can J Bot 56:1806–1808, 1978.Google Scholar
  9. 9.
    Lahoz R, Reyes F, Villanueva P, Jimeno L: Changes in the shape and size of vacuoles during autolysis of Neurospora crassa. Mycopathologia 78:3–10, 1982.Google Scholar
  10. 10.
    Nelson N: A photometric adaptation of the Somogyi method to the determination of glucose. J Biol Chem 153:375–380, 1944.Google Scholar
  11. 11.
    Postgate JR: Death in macrobes and microbes. In: TRG Gray & JR Postgate (Eds), The Survival of Vegetative Microbes, pp. 1–18. The Society for General Microbiology. Symposium 26. Cambridge, Cambridge University Press, 1976.Google Scholar
  12. 12.
    Reyes F, Lahoz R, Vazquez C: Lytic enzymes in the autolysis of Schizophyllum commune with special reference to 1,3-α-glucanase. Can J Microbiol 26:1120–1125, 1980.Google Scholar
  13. 13.
    Riemay K-H, Tröger R: Autolyse bei Pilzen. I. Autolyse bei Coprinus und in Pilzkulturen. Z Allg Mikrobiol 18:523–540, 1978.Google Scholar
  14. 14.
    Riemay K-H, Tröger R: Autolyse bei Pilzen. II. Autolytischer Abbau von Kohlenhydraten, Proteinen und Nucleinsäuren. Z Allg Mikrobiol 18:617–625, 1978.Google Scholar
  15. 15.
    Righelato RC: Growth kinetics of mycelial fungi. In: JE Smith & DR Berry (Eds), The Filamentous Fungi, Vol. 1, pp. 77–103. Edward Arnold, London, 1975.Google Scholar
  16. 16.
    Ritter R: Physiologische Untersuchungen and Zygomyceten. Arch Mikrobiol 22:248–284, 1955.Google Scholar
  17. 17.
    Somogyi M: A new reagent for the determination of sugars. J Biol Chem 160:61–73, 1945.Google Scholar
  18. 18.
    Trinci APJ: Influence of the width of the peripheral growth zone on the radial growth rate of fungal colonies on solid media. J Gen Microbiol 67:325–344, 1971.Google Scholar
  19. 19.
    Trinci APJ, Righelato RC: Changes in constituents and in ultrastructure of hyphal compartments during autolysis of glucose-starved Penicillium chrysogenum. Jour Gen Microbiol 60:239–249, 1970.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1986

Authors and Affiliations

  • R. Lahoz
    • 1
  • F. Reyes
    • 1
  • G. Gómez Alarcón
    • 1
  • L. Cribeiro
    • 1
  • M. A. Junquera
    • 1
  • R. Lahoz-Beltrá
    • 1
  1. 1.Instiluto ‘Jaime Ferrán’ de Microbiología, del Consejo Superior de Investigaciones CientíficasMadridSpain

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