, Volume 82, Issue 2, pp 65–76 | Cite as

Biological, physical and chemical changes in stored wheat

  • H. A. H. Wallace
  • P. L. Sholberg
  • R. N. Sinha
  • W. E. Muir


Six lots of wheat from six farmers' bins in Manitoba were adjusted to a range of 5 moisture contents, held at 6 temperatures and sampled at 6 times during storage. After sampling, seeds were surface sterilized with mercuric chloride and subsamples plated on Czapek's agar, on filter paper moistened with water, or on filter paper moistened with a 7.5% sodium chloride solution. The microflora on the seed was determined, germination counts were made, and the seed was examined by government inspectors for its condition and grade. A total of 1192 samples were examined. In addition, 180 samples were subjected to a fat acidity test. Deterioration for each combination of temperature and moisture, the fungi involved, and the consequent effect on condition, grade and fatty acid content were observed. The effect of high moisture content (>20%) and low temperatures (3–10 ° C) on infection of seed by Penicillium and the consequent effect on germination, condition, grade and fatty acid value are stressed. The interrelationship among fungi, and among fungi and temperature, moisture, storage time, fat acidity values and germination are indicated by correlation coefficient matrices.


Agar Sodium Chloride Filter Paper Acid Content Chloride Solution 
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  1. 1.
    Anonymous. 1962. Cereal laboratory methods. 7th ed. Method 02-01. Fat acidity — general method. Am. Ass. Cereal Chem., St. Paul, MN.Google Scholar
  2. 2.
    Anonymous. 1975. Moisture measurement — grain and seeds. ASAE No. S352, Agric. Engineer's Yearbook. Amer. Soc. Agric. Eng., St. Joseph, Mich.Google Scholar
  3. 3.
    Anonymous. 1979. Canadian grains industry statistical handbook 79. Canada Grains Council, Winnipeg, Canada. 269 pp.Google Scholar
  4. 4.
    Christensen, C. M. & Kaufman, H. H., 1969. Grain storage. The role of fungi in quality loss. Univ. Minn. Press. Minneapolis. 154 pp.Google Scholar
  5. 5.
    Christensen, C. M., 1980. Needed: research on storage molds in grain seeds, and their products. Plant Disease 64: 1067–1070.Google Scholar
  6. 6.
    Dodds, M. E. & Warder, G. F., 1970. The combining and drying of high moisture spring wheat. Can. J. Plant Sci. 50: 67–70.Google Scholar
  7. 7.
    Hurd, A. M., 1921. Seed coat injury and viability of seeds of wheat and barley as factors in susceptibility to moulds and fungicides. J. Agr. Res. 21: 99–122.Google Scholar
  8. 8.
    Jensen, L. B., 1948. Mustiness in foods. J. Food Sci. 5: 89–93.Google Scholar
  9. 9.
    Machaceck, J. E., 1950. An agar-sheet method of testing the efficiency of seed treating machines. Can. J. Res. C 28: 739–744.Google Scholar
  10. 10.
    Machaceck, J. E., Cherewick, W. J., Mead, H. W. & Broadfoot, W. C., 1951. A study of seed-borne diseases of cereals in Canada. II. Kinds of fungi and prevalence of disease in cereal seed. Scient. Agric. 31: 193–206.Google Scholar
  11. 11.
    Machacek, J. E., Robertson, E., Wallace, H. A. H. & Phillips, N. Agnes, 1961. Effect of a high water content in stored wheat, oat and barley seed on its germinability, susceptibility to invasion by moulds, and response to chemical treatment. Can. J. Plant. Sci. 41: 288–303.Google Scholar
  12. 12.
    Mills, J. T., Sinha, R. N. & Wallace, H. A. H., 1978. Multivariate evaluation of isolation techniques for fungi associated with stored rapeseed. Phytopath. 68: 1520–1525.Google Scholar
  13. 13.
    Muir, W. E., 1980. Grain drying and storage in Canada. Journ. Soc. Agric. Structures, Japan. 10: 91–105.Google Scholar
  14. 14.
    Pelhate, J., 1968. Contribution à l'étude écologique des moississures des blés de conservation. (Contribution to the ecological study of moulds of stored wheat). Theses. Univ. Rennes, Brest. 67 pp. (French).Google Scholar
  15. 15.
    Semeniuk, G., 1954. Microflora. Chap. III. p. 77–151. In Storage of cereal grains and their products (Anderson, J. A. & Alcock, A. W., eds.). Amer. Assoc. Cereal Chemists, St. Paul, Minn.Google Scholar
  16. 16.
    Sinha, R. N., Liscombe, E. A. R. & Wallace, H. A. H., 1962. Infestation of mites, insects and microorganisms in a large wheat bulk after prolonged storage. Can. Ent. 94: 542–555.Google Scholar
  17. 17.
    Sinha, R. N. & Wallace, H. A. H., 1965. Ecology of a fungus-induced hot spot in stored grain. Can. J. Plant Sci. 45: 48–59.Google Scholar
  18. 18.
    Sinha, R. N., 1979. Ecology of microflora in stored grain. Ann. Technol. Agric. 28: 191–209.Google Scholar
  19. 19.
    Stawicki, S., Hamenski, E., Niewearowicz, A., Trojan, M. & Wasowicz, E., 1973. The effect of microflora on the formation of odours in grain during storage. pp. 309–336. In Preservation of wet harvested grain (Multon, J. L. & Guilbout, A., eds.). Institut National de la Recherche Agronomique, Paris.Google Scholar
  20. 20.
    Steel, R. G. D. & Torrie, J. H., 1960. Principles and procedures of statistics. McGraw-Hill.Google Scholar
  21. 21.
    Torrow, Elizabeth, 1932. Einwirkung und Nachweis des Quecksilbers bei der Beizung des Saatguts. Phytopath. Z. 4: 631–637.Google Scholar
  22. 22.
    Trisvyatskii, L. A., 1966. Kranenic zerna (Storage of Grain). 407 pp. (Russian). English translation by Keane, D. M., Vols. 1–3. 1969. Natl. Lending Library Sci. Technol., Boston Spa, England, 845 pp.Google Scholar
  23. 23.
    Wallace, H. A. H. & Sinha, R. N., 1962. Fungi associated with hot spots in farm stored grain. Can. J. Plant Sci. 42: 130–141.Google Scholar
  24. 24.
    Wallace, H. A. H. & Machacek, J. E., 1963. Some factors affecting the dispersal of liquid seed dressings among cereal seeds. Can. J. Plant Sci. 43: 313–322.Google Scholar
  25. 25.
    Wallace, H. A. H., 1973. Fungi and other organisms associated with stored grain. Chap. 4, pp. 71–98. In Grain Storage: part of a system (Sinha, R. N. & Muir, W. E., eds.). Avi Publishing Co., Westport, Conn.Google Scholar
  26. 26.
    Wallace, H. A. H., Sinha, R. N. & Mills, J. T., 1976. Fungi associated with small wheat bulks during prolonged storage in Manitoba. Can. J. Bot. 54: 1323–1343.Google Scholar
  27. 27.
    Wallace, H. A. H. & Sinha, R. N., 1981. Causal factors operative in distributional patterns and abundance of fungi: a multi-variance study. In The fungal community, pp. 233–247 (Wicklaw, D. T. & Carroll, G. C., eds.). Marcel Dekker, Inc., N.Y.Google Scholar

Copyright information

© Dr W. Junk Publishers 1983

Authors and Affiliations

  • H. A. H. Wallace
    • 1
  • P. L. Sholberg
    • 2
  • R. N. Sinha
    • 1
  • W. E. Muir
    • 2
  1. 1.Research StationAgriculture CanadaWinnipegCanada
  2. 2.Agricultural Engineering DepartmentUniversity of ManitobaWinnipegCanada

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