Use of enzyme-linked immunosorbent assay for the diagnosis of equine Histoplasmosis farciminosi (epizootic lymphangitis)

Abstract

An enzyme-linked immunosorbent assay was evaluated for the detection of antibody in sera of equine naturally infected with Histoplasma farciminosum ‘epizootic lymphangitis’. Ten sera from naturally infected horses were tested. A hydrogen peroxide ABTS mixture constituted the substrate. The reactions were read as the absorbance values measured at 405 nm using a spectrophotometer. The standard deviation and the average percentage of the absorbance values of the different serum samples were considered in the interpretation of the results. All sera were proved positive with variations in the different samples. Ground preparation of four weeks old growth of the fungus in a phosphate buffer saline was used as antigen. A peroxidase labeled goat anti-equine IgG was used as a conjugate.