Serotyping of fungal isolates by precipitating monoclonal antibodies
Ascites fluid from hybridoma cell lines producing monoclonal antibodies to an exoantigen of Microsporum canis was assayed for its precipitating properties. Three types of monoclonal antibodies were used for serological analysis of heterologous mycelial cultures. Based on specific antigenic determinants, the fungal strains showed different immunodiffusion reactivity within the species. The monoclonal antibodies also proved to be effective reagents for serotyping heterogeneous species. They may also have potential applications in epidemiology.
KeywordsMonoclonal Antibody Potential Application Canis Fungal Strain Fungal Isolate
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- 1.Kaufman L, Standard PG: Improved version of exoantigen test for identification of Coccidioides immitis and Histoplasma capsulatum cultures. J Clin Microbiol 8:42–45, 1978.Google Scholar
- 2.Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1951.Google Scholar
- 3.Palmer DF, Kaufman L, Kaplan W, Cavallaro JJ: Immunodiffusion test for candidiasis. p 44–52, In: A. Balows (ed), Serodiagnosis of mycotic diseases, Charles C Thomas, Publisher, Springfield, Ill., 1978.Google Scholar
- 4.Polonelli L, Morace G: Serological analysis of dermatophyte isolates with monoclonal antibodies produced against Microsporum canis. J Clin Microbiol 21:138–139, 1985.Google Scholar