Mycopathologia

, Volume 97, Issue 3, pp 173–178 | Cite as

Esterogenic effect of zearalenone on the uterine acetyl cholinesterase in female rats

  • A. Y. Nassar
  • S. E. Megalla
  • A. H. Hafez
Article

Abstract

A single dose of zearalenone (10 μg/g LBW) was injected intraperitoneally to Wistar Albino rats at the age of 50–100 days. The uterine acetyl cholinesterase enzyme was significantly increased in the treated animals compared to that in the controls. Therefore, zearalenone would be considered as an esterogenic effector for increasing the uterine acetyl cholinesterase which enhances uterine relaxation and subsequently improves its function for pregnancy in prematured-animals.

Unlike estradiol, it was interesting to find that the estrogenicity of zearalenone was increased by the moderating progesterone hormone. Moreover, it was revealed in this study that the injected dose of zearalenone had no deleterious effects on the pregnant rats at 10–12 days period of gestation. The harmful effects of zearalenone on pregnant animals cited in the literature (11, 13, 19) were reviewed.

Keywords

Enzyme Progesterone Single Dose Estradiol Acetyl 

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References

  1. 1.
    Chang K, Kurtz HJ, Mirocha CJ: Effect of the mycotoxin Zearalenone on Swine reproduction. Am J Vet Res 40:1260–1266, 1979.Google Scholar
  2. 2.
    Chykin S: in Biochemistry Laboratory Technique, p. 114, Wiely Eastern Private Limit. New Delhi, 1970.Google Scholar
  3. 3.
    Eik-Nes K: Physiol Rev 44:609, 1964.Google Scholar
  4. 4.
    Garfield RE, Kannan MS, Daniel EE: Am J Physiol 238 (Cell physiol 7):C.81, 1980.Google Scholar
  5. 5.
    Gomori G: in Microscopic Histochemistry, Chicago, 1952.Google Scholar
  6. 6.
    Greenman DL, Mehata RG, Wittliff JL: Nuclear interaction of Fusarium mycotoxins with esteradiol binding sites in the mouse uterus. J Toxic Environm Hlth 5:593–598, 1979.Google Scholar
  7. 7.
    Ismail M, Ellison AC, Tilmisany AK: Teratogenicity in the rat of the venom from the Scorpion Androctonus amoreuxi (Aud. and Sav.). Toxicon 21:177–189, 1983.Google Scholar
  8. 8.
    Kiang DT, Kennedy BJ, Pather SV, Microcha CJ: Binding characteristics of zearalenone analogs to estrogen receptors. Cancer Res 38:3611–3615, 1978.Google Scholar
  9. 9.
    King RJB, Gordon J, Marx J, Steggles AN: In: Hubinot PO, Leroy F, Garland P (eds) Basic actions of sex steroids on target organs. Kanger press, Basel, 1971, p 21.Google Scholar
  10. 10.
    Kores R, Veld T, Schuller PL, Stephany RW: Environmental quality and safety. In: Lu FC, Rendel J (eds) Anabolic agents in animal production. FAO/WHO symposium, Rome, March 1975, Vol. V (1979) Thieme Verlag. Stuttgart GFR, 1975.Google Scholar
  11. 11.
    Long GG, Diekman MA, Tuite JF, Shannon GM, Vesonder RF: Effect of F. roseum (Gibberella zeae) on pregnancy and the estrous cycle in gilts fed molded corn on days 7–17 post-estrus. Vet Res Commun 6:199–204, 1983.Google Scholar
  12. 12.
    Megalla SE, Bakheit BR: Zearalenone accumulation in Zea mays kernels from a seven-line diallel infected with Gibberella zeae. Seed Science and Tech (in press).Google Scholar
  13. 13.
    Miller JK, Hacking A, Gross VT: Stillbirths, neonatal mortality and small litters in pigs associated with the ingestion of Fusarium toxin by pregnant Sows. Vet Rec 93:555–559, 1973.Google Scholar
  14. 14.
    Ralston AT: Effect of zearalenone on weaning weight of male calves. J Anim Sci 47:1203–1206, 1978.Google Scholar
  15. 15.
    Rothenbacher H, Wiggins JP, Wilson LL: Pathogenic changes in endocrine glands and certain other tissues of lambs implanted with the synthetic growth promotant zearalenol. Am J Vet Res 36:1313–1316, 1975.Google Scholar
  16. 16.
    Thouvenot D, Morfin RF: Interferences of zearalenone, zearalenol, or estradiol-17B with the steroid-metabolizing enzymes of the human prostate gland. J Steroid Biochem 13:1337–1345, 1980.Google Scholar
  17. 17.
    Ueno Y, Shimada N, Yagasaki S, Enomoto M: Toxicological approaches to the metabolites of Fusaria. VII. Effects of zearalenone on the uteri of mice and rats. Chem Pharm Bull 22:2830–2835, 1974.Google Scholar
  18. 18.
    Ueno Y, Yagasaki S: Accelerating effect of zearalenone on RNA and protein synthesis in the uterus of ovariectomized mice. Japan J Exp Med 45 (3):199–205, 1975.Google Scholar
  19. 19.
    Weaver GA, Kurtz HJ, Mirocha CJ, Bates FY, Behrens JC, Robinson TS, Gipp WF: Mycotoxin induced abortions in swine. Can Vet J 19:72–74, 1978a.Google Scholar
  20. 20.
    Wiggins JP, Rothenbacher H, Wilson LL, Martin RJ, Wangsness BJ, Ziegler JH: Growth and endocrine responses of lambs to zearalenol implants: Effects of preimplant growth rate and of sire. J Anm Sci 49:291–297, 1979.Google Scholar

Copyright information

© Martinus Nijhoff/Dr W. Junk Publishers 1987

Authors and Affiliations

  • A. Y. Nassar
    • 1
  • S. E. Megalla
    • 1
  • A. H. Hafez
    • 1
  1. 1.Departments of Biochemistry, Botany and Animal HygieneUniversity of AssiutAssiutEgypt

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