Skip to main content
Log in

Agressivité de Conidiobolus obscurus vis à-vis du puceron du pois

II. Mode de germination in vitro des conidies primaires de souches d'agressivité différente

  • Published:
Mycopathologia Aims and scope Submit manuscript

Résumé

L'influence de différents facteurs physiques (température, pH, lumière, pression osmotique) et nutritionnels (sels, vitamines, sucres et hydrolysats de protéine) a été étudiée sur la germination des conidies primaires de Conidiobolus obscurus. Les germinations globales les plus élevées sont obtenues à 24 °C, à la lumière sur un milieu gélosé contenant du glucose et des vitamines ou de l'extrait de levure. Une température comprise entre 19 et 24 °C, un pH basique (8–10), l'obscurité et la présence de glucose et/ ou d'hydrolysats de protéine stimulent l'émission de tubes germinatifs. Les souches ont pu être classées en 2 groupes en fonction de leur mode de germination in vitro et de leur agressivité vis-à-vis du puceron du pois.

Abstract

Different physical (temperature, pH, light and osmotic pressure) and nutritional factors (salts, vitamins, sugars and protein hydrolysates) were tested on the germination of primary conidia of Conidiobolus obscurus. The most important total germination levels were obtained at 24 δC, in continuous light on an agar medium containing dextrose and vitamins or yeast extract. Temperatures ranging from 19 to 24°, a basic pH (8–10), darkness and the presence of dextrose and/ or protein hydrolysates stimulated the formation of germ tubes. The strains of C. obscurus studied were classified in 2 groups depending on their aggressivity against the pea aphid and their mode of germination in vitro.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Allen, P. J., 1976. Spore germination and its regulation. Control of spore germination and infection structure formation in fungi. In: Encyclopedia of Plant Physiology, Vol. 4. Physiological Plant Pathology (Heitefuss, R. & Williams, P. H., eds.), pp. 53–85. Springer Verlag, Berlin.

    Google Scholar 

  2. Auclair, J. L., 1958. Honeydew excretion in the pea aphid, Acyrthosiphon pisum (Harr.) (Homoptera: Aphididae). J. Ins. Physiol. 2: 330–337.

    Google Scholar 

  3. Ben Ze'ev, I., 1980. Systematics of entomopathogenic fungi of the ‘Sphaerosperma’ group and their prospects for use in biological pest control. Ph.D. Thesis. Univ. Jerusalem.

  4. Callaghan, A. A., 1974. Effect of pH and light on conidium germination in Basidiobolus ranarum. Trans. Br. Mycol. Soc. 63: 13–18.

    Google Scholar 

  5. Callaghan, A. A., 1978. Effect of nutrient level, pH and light on conidial germination in Entomophthoraceous fungi. Trans. Br. Mycol. Soc. 70: 271–275.

    Google Scholar 

  6. Kerwin, J. L., 1982. Chemical control of the germination of asexual spores of Entomophthora culicis, a fungus parasitic on Dipterous. J. Gen. Microbiol. 128: 2179–2186.

    Google Scholar 

  7. Latgé, J. P., 1975. Croissance et sporulation de 6 espèces d'Entomophthorales. 1. Influence de la nutrition carbonée. Entomophaga 20: 201–207.

    Google Scholar 

  8. Latgé, J. P., 1981. Comparaison des exigences nutritionnelles des Entomophthorales. Ann. Microbiol. (Inst. Pasteur) 132B: 299–306.

    Google Scholar 

  9. Latgé, J. P., 1983. Conidiobolus obscurus et les Entomophthorales pathogènes de pucerons. Thèse Doctorat d'Etat, Université Paris XI-Orsay, 7 mars 1983, 2 vols.

  10. Latgé, J. P., Papierok, B. & Sampedro, L., 1982. Agressivité de Conidiobolus obscurus vis-à-vis du puceron du pois. I. Comportement des conidies sur la cuticule avant la pénétration du tube germinatif dans l'insecte. Entomophaga (sous presse).

  11. Latgé, J. P., Sampedro, L. & Brey, P., 1982. Physiological study of the pathogenicity of Conidiobolus obscurus. Invertebrate Pathology and Microbial Control. Abstr. Offered papers. 3rd int. coll. Brighton, 6–10 September 1982, p. 109.

  12. Page, R. M. & Humber, R. A., 1973. Phototropism in Conidiobolus coronatus. Mycologia 65: 335–354.

    Google Scholar 

  13. Papierok, B. & Wilding, N., 1979. Mise en évidence d'une différence de sensibilité entre 2 clones du puceron du pois, Acyrthosiphon pisum Harr. (Homoptères Aphididae) exposés à 2 souches du champignon Phycomycète: Entomophthora obscura Hall & Dunn. C.R. Acad. Sc. Paris, série D 288: 93–95.

    Google Scholar 

  14. Pekrul, S. & Grula, E. A., 1979. Mode of infection of the corn ear worm (Heliothis zea) by Beauveria bassiana as revealed by scanning electron microscopy. J. Invertebr. Pathol. 34: 238–247.

    Google Scholar 

  15. Preece, T. F., 1976. Some observations on leaf surface during the early stages of infection by fungi. In: Biochemical aspects of Plant-Parasite Relationships (Friend, J. & Threlfall, D. K., eds.), pp. 1–10. Academic Press, London & New York.

    Google Scholar 

  16. Remaudière, G., Latgé, J. P. & Papierok, B., 1979. Reconsidération taxonomique de Entomophthora obscura Hall & Dunn. Ann. Microbiol. (Inst. Pasteur) 130A: 151–162.

    Google Scholar 

  17. Schabel, H. G., 1978. Percutaneous infection of Hylobius pales by Metarrhizium anisopliae. J. Invertebr. Pathol. 31: 180–187.

    Google Scholar 

  18. Smith, R. J. & Grula, E. A., 1981. Nutritional requirements for conidial germination and hyphal growth of Beauveria bassiana. J. Invertebr. Pathol. 37: 222–230.

    Google Scholar 

  19. Uziel, A., Schwartz, A. & Kenneth, R. G., 1981. Positive phototropism and hydromorphogenesis in germination structures of Conidiobolus species. Isr. J. Botany 30: 75–80.

    Google Scholar 

  20. Uziel, A., 1981. The effect of light regimes and substrates on the mode of germination of conidia of certain fungi of the Entomophthorales (Phycomycetes: Zygomycetes). M. Sc. Thesis, Faculty of Agriculture, Rehovot, Israel.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sampedro, L., Uziel, A. & Latgé, J.P. Agressivité de Conidiobolus obscurus vis à-vis du puceron du pois. Mycopathologia 86, 3–19 (1984). https://doi.org/10.1007/BF00437224

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00437224

Navigation