Abstract
The present study reproduced the experimental model of ocular paracoccidioidomycosis in guinea pigs, by the intracardiac inoculation of yeast-forms of P. brasiliensis. Ocular involvement was observed in 80% of the infected animals. The uvea, ciliary body, choroid, iris, lids and the conjunctiva were the structures most commonly affected. To protect the animals against the infection, an immunization protocol was standardized utilizing a P. brasiliensis soluble antigen in Freund's complete adjuvant, administered weekly, during 3 weeks, by the subcutaneous route. Two weeks later, previously immunized guinea pigs were challenged by the intracardiac route with yeast-forms of P. brasiliensis (vaccinated group). When compared with a control group (infection in the absence of prior immunization), the vaccinated animals developed higher levels of anti-P. brasiliensis cellular and humoral immune response and a three times lower frequency of ocular involvement (85.7% vs 28.5%). In addition, the ocular lesions were significantly more localized and contained less fungal cells. The data demonstrated that the subcutaneous immunization was effective in decreasing the frequency and extent of ocular lesions, as well as in blocking fungal multiplication.
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References
Almeida F. Vacina contra o granuloma paracoccidioidico. Folia Clin Biol (S Paulo) 1938; 10:195–197.
Arango M, Oropeza P, Anderson O, Contreras C, Bianco N, Yarzabal L. Circulating immune complexes and in vitro cell reactivity in paracoccidioidomycosis. Mycopathologia 1982; 79:153–158.
Arruda WO, Canto MAS, Loddo G, Rebuffi VF, Cardoso MA. Paracoccidioidomicose ocular. Relato de 1 caso com coriorretinite posterior. Rev Inst Med Trop S Paulo 1986; 28:190–193.
Biagioni L, Souza MJ, Chamma LG, Mendes RP, Marques SA, Mota NGS, Franco M. Serology of paracoccidioidomycosis. II. Correlation between class-specific antibodies and clinical forms of the disease. Trans Roy Soc Trop Med Hyg 1984; 78:617–621.
Brito T, Fava Netto C. Disseminated experimental South American blastomycosis of the guinea pig, a pathologic and immunologic study. Path Microbiol 1963; 26:29–43.
Burger E, Singer-Vermes LM, Calich VLG. The role of C5 in experimental murine paracoccidioidomycosis. J Infect Dis 1985; 152:425.
Calich VLG, Purchio A, Paulo CR. A new fluorescent viability test for fungi cells. Mycopathologia 1978; 66:175–177.
Calich VLG, Kipnis TL, Mariano M, Favo Netto C, Dias da Silva W. The activation of the complement system by Paracoccidioides brasiliensis in vitro: its opsonic effect and possible significance for an in vivo model of infection. Clin Immunol Immunopath 1979; 12:20–30.
Carvalhaes MS, Silva WD, Birman EG, Sant'anna OA, Abrahamsohn P, Kipnis TL. Experimental paracoccidioidomycosis in high and low antibody-producer mice. I. Evolution of the disease, its correlation with the humoral immune response and the patterns of tissue lesions. Ann Inst Pasteur/Immun 1986; 137:127–141.
Conti-Diaz IA. Lesiones oculares en la blastomicosis sudamericana. Hospital (Rio de Janeiro) 1960; 25:903–914.
Fava Netto C. Imunologia da paracoccidioidomicose. Rev Inst Med Trop S Paulo 1976; 18:42–53.
Fava Netto C, Raphael A. A reação intradèrmica com polissacàride do Paracoccidioides brasiliensis, na blastomicose sul americana. Rev Inst Med Trop S Paulo 1961; 3:161–65.
Fava Netto C, Vegas VS, Sciannamèa IM, Guarnieri DB. Antigeno polissacaridico do Paracoccidioides brasiliensis. Estudo do tempo de cultivo do P. brasiliensis necessario ao preparo do antigeno. Rev Inst Med Trop S Paulo 1969; 11:177–181.
Franco M. Host-parasite relationship in paracoccidioidomycosis. J Med Vet Mycol 1987; 25:5–18.
Goihman-Yahr M, Essenfeld-Yahr E, Albornoz M, Yarzàbal L, Gomes MH, Martin BS, Ocanto A, Gil F, Convit J. Defect of in vitro digestive ability of polymorphonuclear leukocytes in paracoccidioidomycosis. Infect Immun 1980; 28:557–566.
Grocott RG. Gomori's application of Gomori's methenamine silver nitrate technic to fungi. Am J Clin Path 1955; 25:975–979.
Jimenez BE, Murphy JW. In vitro effects of natural killer cells against Paracoccidioides brasiliensis yeast phase. Infect Immun 1984; 46:552–558.
Lowry OH, Rosembourg NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193:265–275.
Marques SA, Franco MF, Mendes RP, Silva NCA, Bacchi C, Curcelli ED, Feracin ACM, Oliveira CS, Tagliarini JV, Dillon NL. Aspectos epidemiologicos da paracoccidioidomicose na àrea endêmica de Botucatu (São Paulo —Brasil). Rev Inst Med Trop S Paulo 1983; 25:87–92.
Mendes E, Raphael A. Impaired delayed hypersensitivity in patients with South American blastomycosis. J Allergy 1971; 47:17–22.
Mendes NF, Musatti CC, Leão RC, Mendes E, Naspitz CK. Lymphocyte cultures and skin allograft survival in patients with South American blastomycosis. J Allergy Clin Immunol 1971; 48:40–45.
Miyaji M, Nishimura K. Granuloma formation and killing. functions of granuloma in congenitally athymic nude mice infected with Blastomyces dermatitidis and Paracoccidioides brasiliensis. Mycopathologia 1983; 82:129–141.
Moscardi-Bacchi MM, Franco M. Experimental paracoccidioidomycosis in the mouse. III. Histopathological and immunological findings after intravenous infection in the presence or absence of previous immunization. Rev Soc Bras Med Trop 1985; 18:101–108.
Mota NGS, Rezkallah-Iwasso MT, Peraçoli MTS, Audi RG, Mendes RP, Marcondes J, Marques SA, Dillon NL, Franco MF. Correlation between cell-mediated immunity and clinical forms of paracoccidioidomycosis. Trans Roy Soc Trop Med Hyg 1985; 79:765–772.
Musatti CC, Rezkallah-Iwasso MT, Mendes E, Mendes NF. In vivo and in vitro evaluation of cell-mediated immunity in patients with paracoccidioidomycosis. Cell Immunol 1976; 24:365–378.
Negroni R, Iovannitti C, Costa MRIE, Gonzàlez JL. Paracoccidioidomicosis diseminada experimental del cobayo. Rev Argent Micol 1979; 2:5–10.
Peraçoli MTS, Mota NGS, Montenegro MR. Experimental paracoccidioidomycosis in the Syrian hamster, morphology and correlation of lesions with humoral and cell-mediated immunity. Mycopathologia 1982; 79:7–17.
Restrepo A, Restrepo M, Restrepo I, Aristizàbal LH, Moncada LH, Velez H. Immune responses in paracoccidioidomycosis. A controlled study of 16 patients before and after treatment. Sabouraudia 1978; 16:151–163.
Robledo MA, Graybill JR, Ahrens J, Restrepo A, Drutz DJ, Robledo M. Host defense against experimental paracoccidioidomycosis. Am Rev Resp Dis 1982; 25:563–567.
Salvin SB, Neta R. Immunopathology of mycotic infections. in: Rose NR & Siegel BV. Immunopathology. New York: Plenum, 1983:145–199.
Siegel S. Estatìstica não paramètrica. Sao Pãulo: McGraw Hill, 1975:350.
Terra F. Três casos de blastomycose. Brasil Med 1923; 1:41–44.
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Kamegasawa, A., Viero, R.M., Rezkallah-Iwasso, M.T. et al. Protective effect of prior immunization on ocular paracoccidioidomycosis in guinea pigs. Mycopathologia 103, 35–42 (1988). https://doi.org/10.1007/BF00437219
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DOI: https://doi.org/10.1007/BF00437219