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Mycopathologia

, Volume 118, Issue 3, pp 167–171 | Cite as

Effect of carbon dioxide on sporulation of Alternaria crassa and Alternaria cassiae

  • M. G. Smart
  • K. M. Howard
  • R. J. Bothast
Plant Mycology and Crop Protection

Abstract

Biological herbicides will assume greater importance for weed control as the number of chemicals registered for use decreases. Two promising fungi pathogenic on Datura stramonium and Cassia obtusifolia are, respectively, Alternaria crassa and Alternaria cassiae. In order to develop technologies for the efficient production of spore inocula in submerged liquid culture, we studied the effect of volatile factors which influenced sporulation on solid media. Sporulation was quantified on Richard's V8 agar under individual and joint variation of carbon dioxide (CO2) and relative humidity (RH). Sporulation of both fungi was suppressed when the two factors increased but not when CO2 was absorbed and RH remained high. Incubation of cultures in a CO2 controlled cabinet with RH constant indicated that CO2 was the suppressant.

Key words

Biological control mycoherbicide fungi weeds 

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References

  1. 1.
    Templeton GE, TeBeest DO, Smith RJ Jr. Development of an endemic fungal pathogen as a mycoherbicide for biocontrol of northern jointvetch in rice, p. 214–220. In: Freeman, PE. ed. Proc. IV 1st Symp Biol Control of Weeds. Gainesville: Univ of Florida 1976; 214–220.Google Scholar
  2. 2.
    Templeton GE, TeBeest DO, Smith RJ. Jr. Biological weed control with mycoherbicides. Ann Rev Phytopathology 1979; 17: 301–310.Google Scholar
  3. 3.
    Boyette CD. Evaluation of Alternaria crassa for biological control of jimsonweed: host range and virulence. Plant Science 1986; 45: 223–228.Google Scholar
  4. 4.
    Walker HC, Riley JA. Evaluation of Alternaria cassiae for the biocontrol of sicklepod (Cassia obtusifolia). Weed Sci1982; 30: 651–654.Google Scholar
  5. 5.
    Walker HC. Alternaria macrospora as a potential biocontrol agent for spured anoda: Production of spores for field studies. Science and Education Administration, Advances in Agricultural Technology, Southern Series, #12. 1980; 5 pp.Google Scholar
  6. 6.
    Cotty PJ, Misaghi IJ. Effect of light on the behavior of Alternaria tagetica in vitro and in vivo. Phytopathology 1985; 75: 366–370.Google Scholar
  7. 7.
    Vakalounakis DJ. Action spectrum of photoinduced conidiation in Alternaria cichorii. J Gen Microbiol 1986; 132: 3485–3489.Google Scholar
  8. 8.
    Vakalounakis DJ, Christias C. Light quality, temperature and sporogenesis in Alternaria cichorii. Trans Br Mycol Soc 1986; 86: 247–254.Google Scholar
  9. 9.
    Cotty PJ. Modulation of sporulation of Alternaria tagetica by carbon dioxide. Mycologia 1987; 79: 508–513.Google Scholar
  10. 10.
    Rowell JB. Controlled infection by Puccinia graminis tritici under artificial conditions. In: Bushnell WR, Roelf AP. ed. The cereal rusts: origins, species, structure and physiology. Academic Press, 1984: 546.Google Scholar
  11. 11.
    Snedecor, GW, Cochran WG. Statistical Methods, 6th ed. Ames: Iowa State Univ Press, 1967: 130.Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • M. G. Smart
    • 1
  • K. M. Howard
    • 1
  • R. J. Bothast
    • 1
  1. 1.Fermentation Biochemistry Research, National Center for Agricultural Utilization ResearchUSDA/ARSPeoriaUSA

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