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A semi-defined medium for culturing Nomuraea rileyi

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  • Plant Mycology and Crop Protection
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Abstract

A semi-defined medium was successfully used to culture several natural and mutant isolates of the entomopathogenic fungus, Nomuraea rileyi. The medium contains only two complex ingredients at very low levels, yet permits germination and mycelial growth of N. rileyi. The medium also facilitates recovery and detection of hydrolytic enzymes. Use of the formulation could simplify nutritional, biochemical and physiological studies with N. rileyi and also might be used to culture other entomopathogenic fungi.

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References

  1. Bell JV. Production and pathogenicity of the fungus Spicaria rileyi from solid and liquid media. J Invertebr Pathol 1975; 26: 129–130.

    Google Scholar 

  2. Boucias DG, Pendland JC. Nutritional requirements for conidial germination of several host range pathotypes of the entomopathogenic fungus Nomuraea rileyi. J Invertebr Pathol 1984; 288–292.

  3. Coudron TA, Kroha MJ, Ignoffo CM. Levels of chitinolytic activity during development of three entomopathogenic fungi. Comp Biochem Physiol 1984; 79B: 339–348.

    Google Scholar 

  4. Coudron TA, Kroha MJ, El-Sayed GN. A novel semiliquid technique for propagating entomopathogenic fungi. J Invertebr Pathol 1985; 46: 335–336.

    Google Scholar 

  5. Holdom DG, Van De Klashorst G. Inexpensive culture media and methods for Nomuraea rileyi. J Invertebr Pathol 1986; 48: 246–248.

    Google Scholar 

  6. Bidochka MJ, Khachatourians GG. Purification and properties of an extracellular protease produced by the entomopathogenic fungus Beauveria bassiana. Appl Environ Microbiol 1987; 53; 1679–1684.

    Google Scholar 

  7. St. Leger RJ, Charnley AK, Cooper RM. Cuticle-degrading enzymes of entomopathogenic fungi: Synthesis in culture on cuticle. J Invertebr Pathol 1986; 48: 85–95.

    Google Scholar 

  8. Leibovitz A. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am J Hyg 1963; 78: 173–180.

    Google Scholar 

  9. McIntosh AH, Maramorosch K, Rechtoris C. Adaptation of an insect cell line (Ag allia constricta) in a mammalian cell culture medium. In Vitro 1973; 8: 375–378.

    Google Scholar 

  10. Munderloh UG, Kurtte TJ. Malarial parasites complete sporogony in axenic mosquitoes. Experientia 1985; 41; 1205–1207.

    Google Scholar 

  11. Tatum EL, Barrat R, Fries N, Bonner D. Biochemical mutant strains of Neurospora produced by physiological and chemical treatment. Am J Bot 1950; 37: 38–46.

    Google Scholar 

  12. El-Sayed GN, Ignoffo CM, Leathers TD. Effects of cuticle source and concentration on germination of conidia of two isolates of Nomuraea rileyi. Mycopathologia1991; 113: 95–102.

    Google Scholar 

  13. Ignoffo CM, Garcia C. Host spectrum and relative virulence of an Ecuadoran and Mississippian biotype of Nomuraea rileyi. J Invertebr Pathol 1985; 45: 346–352.

    Google Scholar 

  14. El-Sayed GN, Coudron TA, Ignoffo CM, Riba G. Chitinolytic activity and virulence associated with native and mutant isolates of an entomopathogenic fungus, Nomuraea rileyi. J Invertebr Pathol 1989; 54: 394–403.

    Google Scholar 

  15. Bidochka MJ, Khachatourians GG. Regulation of extracellular protease in the entomopathogenic fungus Beauveria bassiana. Exp Mycol 1988; 12: 161–168.

    Google Scholar 

  16. Bidochka MJ, Khachatourians GG. N-acetyl-D-glucosamine-mediated regulation of extracellular protease in the entomopathogenic fungus, Beauveria bassiana. Appl Environ Microbiol 1988; 54: 2699–2704.

    Google Scholar 

  17. St. Leger RJ, Cooper RM, Charnley AK. Cuticle-degrading enzymes of entomopathogenic fungi: Regulation of production of chitinolytic enzymes. J Gen Microbiol 1986; 132: 1509–1517.

    Google Scholar 

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This article reports the results of research only. Mention of a proprietary product does not constitute an endorsement or a recommendation for its use by USDA.

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El -Sayed, G.N., Ignoffo, C.M., Leathers, T.D. et al. A semi-defined medium for culturing Nomuraea rileyi . Mycopathologia 118, 163–165 (1992). https://doi.org/10.1007/BF00437149

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  • DOI: https://doi.org/10.1007/BF00437149

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