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Factors involved in the adherence of Candida albicans and Candida tropicalis to protein-adsorbed surfaces

An in vitro study using immobilized protein

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Abstract

The adherence of Candida albicans and C. tropicalis to protein-adsorbed surfaces was investigated with surface-modified glass slides to which serum or salivary proteins were covalently bound. A specific adherence like a ligand-receptor interaction was observed between C. albicans and mucin- or salivary protein-immobilized glass slides. This interaction was eliminated by deglycosylation of the slides, suggesting that the receptor may be an oligosaccharide(s) contained mucin or saliva. A similar specific interaction was also observed between C. tropicalis and fibrinogen-immobilized glass surfaces. When the numbers of adherent cells to deglycosylated protein-immobilized glass glides were plotted against zeta potentials and contact angles of these protein-immobilized glass slides, a significant correaltion was observed between the numbers of adherent cells and zeta potentials in the case of C. albicans (r = −0.87), whereas a significant correlation was observed between cell numbers and contact angles (r = 0.82) in the case of C. tropicalis. These results suggest that the forces governing the adherence of fungi to pellicle in dentures may vary depending upon the surface properties of fungi and substrate.

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Authors and Affiliations

  1. Department of Prosthetic Dentistry, Hiroshima University, School of Dentistry, 1-2-3 Kasumi Minami-ku, 734, Hiroshima, Japan

    Hiroki Nikawa, Shinsuke Sadamori & Taizo Hamada

  2. Department of Clinical Chemistry, Huddinge University Hospital, Karolinska Institute, S-141 86, Huddinge, Sweden

    Kyuichiro Okuda

Authors
  1. Hiroki Nikawa
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  2. Shinsuke Sadamori
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  3. Taizo Hamada
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  4. Kyuichiro Okuda
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Nikawa, H., Sadamori, S., Hamada, T. et al. Factors involved in the adherence of Candida albicans and Candida tropicalis to protein-adsorbed surfaces. Mycopathologia 118, 139–145 (1992). https://doi.org/10.1007/BF00437146

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  • DOI: https://doi.org/10.1007/BF00437146

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