Abstract
To evaluate the rate at which the four main aflatoxins (aflatoxins B1, B2, G1 and G2) are able to cross the luminal membrane of the rat small intestine, a study about intestinal absorption kinetics of these mycotoxins has been made. In situ results obtained showed that the absorption of aflatoxins in rat small intestine is a very fast process that follows first-order kinetics, with an absorption rate constant (k a ) of 5.84±0.05 (aflatoxin B1), 4.06±0.09 (aflatoxin B2), 2.09±0.03 (aflatoxin G1) and 1.58±0.04 (aflatoxin G2) h−1, respectively.
Similar content being viewed by others
References
Betina V. Biological effects of mycotoxins. In: Betina V (ed), Mycotoxins: Chemical, Biological and Environmental Aspects. Amsterdam: Elsevier Science Publishers, 1989: 42–58.
Robens JF, Richard JL. Aflatoxins in animal and human health. Rev Environ Contam Toxicol 1992; 127: 69–94.
Samarajeewa V, Sen AC, Cohen MD, Wey CI. Detoxification of aflatoxins in foods and feeds by physical and chemical methods. J Food Prot 1990; 53: 489–501.
Huff WE, Kubena LF, Harvey RB, Phillips TD. Efficacy of hydrated sodium calcium aluminosilicate to reduce the individual and combined toxicity of aflatoxin and ochratoxin A. Poultry Sci 1992; 71: 64–69.
Harvey RB, Kubena LF, Elissalde MH, Phillips TD. Efficacy of zeolitic ore compounds on the toxicity of aflatoxin to growing broiler chickens. Avian Dis 1993; 37: 67–73.
Kumagai S. Intestinal absorption and excretion of aflatoxins in rats. Toxicol Appl Pharmacol 1988; 97: 88–97.
Association of Official Analytical Chemists, Book of Methods 1990, 15th ed. Arlington, Virginia AOAC, 1990: Sec. 976.22. AACC-AOAC Method.
Doluisio JT, Billups NF, Dittert, LW, Sugita ET, Swintosky JV. Drug absorption. I. An in situ rat gut technique yielding realistic absorption rates. J Pharm Sci 1969; 58: 1196–1199.
Martin-Villodre A, Pla-Delfina JM, Moreno J, Perez-Buendia MD, Miralles-Mir J, Collado EF, Sanchez-Moyano E, Del Pozo A. Studies on the realiability of a bihyperbolic functional absorption model. I. Ring-substituted anilines. J Pharmacokin Biopharm 1986; 14: 615–633.
Merino M, Peris-Ribera JE, Torres-Molina F, Sanchez-Pico A, Garcia-Carbonell MC, Casabo VG, Martin-Villodre A, Pla-Delfina JM. Evidence of a specialized transport mechanism for the intestinal absorption of baclofen. Biopharm Drug Dispos 1989; 10: 279–297.
Merino M, Polache A, Peris-Ribera JE, Moreno-Dalmau J, Pla-Delfina JM. Absorption kinetics of ß-alanine as model compound in rat small intestine. Biochim Biophys Acta 1989; 989: 1–7.
Gabus C, Buri P. Étude comparative des méthodes de détérmination du volume d'eau absorbé lors de perfusion de l'intestine grêle du rat. S.T.P. Pharma 1987; 11: 856–860.
Ramos AJ. Prevención de los efectos cancerígenos de las aflatoxinas y la zearalenona mediante el empleo de compuestos adsorbentes no nutritivos. Estudios in vitro. Ph.D. Thesis, Department of Biotechnology, Polytechnic University of Valencia, Valencia, Spain, 1994.
Thompson MC, Dutton MF, Bye SN, Dehrmann FM. An investigation into the passage of selected natural toxins across the digestive tract wall using the everted sac technique. J. Natural Toxins 1992; 1: 9–16.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ramos, A.J., Hernández, E. In situ absorption of aflatoxins in rat small intestine. Mycopathologia 134, 27–30 (1996). https://doi.org/10.1007/BF00437049
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00437049