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Rapid, economical qualitative method for separation of aflatoxins B-1, B-2 & G-1, G-2 by dry column chromatography

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Abstract

A good resolution of four components of aflatoxins was accomplished by using the dry column chromatography method. The decolorization process of interfering substances, by 0.01 N KOH and defatting the extract with petroleum ether yields a clean residue for DCC separation. It is clear that the dry column chromatography is a very simple and time-saving procedure for separation of aflatoxins. DCC columns are more economical than precoated ‘thick layer’ preparative plates and, in DCC, no large developing tanks need to be used. Hazards associated with the use of large volumes of flammable solvents are greatly reduced.

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References

  1. Abdel-Fattah, H. M., Kamel, Y. Y., Megalla, S. E. & Hafez, A. H., 1982. Aflatoxins and aflatoxicosis I. Fungal flora of some food and animal feeds with special references to aflatoxin-producing abilities. Mycopathologia 77(3): 129–136.

    Google Scholar 

  2. Campbell, A. D. & Funkhower, J. T., 1966. J. Assoc. Offici. Agric. Chem. 49(3): 554–562.

    Google Scholar 

  3. Chen, S. & Fridman, L., 1966. J. Assoc. Offici. Agric. Chem. 49(1): 28–33.

    Google Scholar 

  4. Coomes, T. J., Crowther, P. C., Francis, B. J. & Stevens, L., 1965. Analyst 90: 429–496.

    Google Scholar 

  5. Gimeno, A., 1979. Thin layer Chromatographic determination of aflatoxins, ochratoxins, sterigmatocystin, zearalenone, citrinin, T-2 toxin, diacetoxyscripenol, penicillic acid, patulin, penitrem A. J. Assoc. Offici. Anal. Chem. 62(3): 579–585.

    Google Scholar 

  6. Goldblatt, L. A., 1967. U.S. Department of Agriculture, mycotoxin seminar, Washington D.C., June 16, 1967.

  7. Loev, B. & Snader, K. M., 1965. Chem. Ind. (London) 15.

  8. Loev, B. & Goodman, M. M., 1967. Chem. Ind. (London) 2026.

  9. Mislivec, P. B., Hunter, J. H. & Tuite, J., 1968. Assay for aflatoxin production by the genera Aspergillus and Penicillium. Appl. Microbiol. 16(7): 1053–1055.

    Google Scholar 

  10. Nabeny, J. & Nesbitt, B. F., 1965. A spectrophotometric method for determining the aflatoxins. Analyst 90: 155–160.

    Google Scholar 

  11. Pons, W. A., Cucullu, A. F., Lee, L. S., Robertson, J. A., Franz, A. O. & Goldblatt, L. A., 1966. ibid. 49(3): 554–562.

    Google Scholar 

  12. Shotwell, O. L., Hesseltine, C. W., Stubblefield, R. D. & Sorenson, W. G., 1966. Production of aflatoxin on rice. Appl. Microbiol. 14(3): 425–428.

    Google Scholar 

  13. Stubblefield, R. D., Shotwell, O. L. & Shannon, G. M., 1968. Aflatoxins B-1, B-2, G-1, and G-2: separation and purification. J. Am. Oil Chem. Soc. 45(10): 686–688.

    Google Scholar 

  14. Stubblefield, R. D., Shannon, G. M. & Shotwell, O. L., 1969. Aflatoxins: improved resolution by thin layer. J. Assoc. Anal. Chem. 52(4): 669–672.

    Google Scholar 

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Authors and Affiliations

  1. Department of Botany Faculty of Science, Assiut University, Assiut, Egypt

    Samuel E. Megalla

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  1. Samuel E. Megalla
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Megalla, S.E. Rapid, economical qualitative method for separation of aflatoxins B-1, B-2 & G-1, G-2 by dry column chromatography. Mycopathologia 84, 45–47 (1983). https://doi.org/10.1007/BF00436996

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