, Volume 84, Issue 1, pp 17–19 | Cite as

Rapid diagnosis of systemic candidiasis in children with cancer by pyrolysis gas liquid chromatography

  • M. Gunasekaran
  • W. T. Hughes
  • Richard B. Wilber


A pyrolysis gas liquid Chromatographic method for detection of Candida antigen in patients with disseminated candidiasis is described. The test was positive in 73% of cases and no false positives or false negatives were noted in controls.


Chromatography Liquid Chromatography Pyrolysis False Positive Candida 
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  1. 1.
    Brooks, J. B. & Melton, A. R., 1978. Electron capture gas liquid Chromatographic study of metabolites produced by some arthritis transudate-associated organisms in vitro and in vivo in rabbit models. J. Clin. Microbiol. 8: 402–409.Google Scholar
  2. 2.
    Coloe, P. T., 1978. Head-space gas liquid chromatography for rapid detection of E. coli and Proteus mirabilis in urine. J. Clin. Pathol. 31: 365–369.Google Scholar
  3. 3.
    Hughes, W. T., 1973. Infection problems in childhood cancer. In: Seventh National Cancer Conference Proceedings, pp. 601–604. American Cancer Society, J. B. Lippincott Co., Philadelphia.Google Scholar
  4. 4.
    Hughes, W. T., 1976. Infections in the compromised host. Adv. Intern. Med. 22: 73–96.Google Scholar
  5. 5.
    Kiehn, T. E., Bernard, E. M., Gold, J. W. M. & Armstrong, D., 1979. Candidiasis: Detection by gas liquid chromatography of D-arabinitol, a fungal metabolite, in human serum. Science 206: 577–580.Google Scholar
  6. 6.
    Miller, G. G., Witwer, M. W., Braude, A. I. & Davis, C. E., 1974. Rapid identification of Candida albicans septicemia in man by gas-liquid chromatography. J. Clin. Invest. 54: 1235–1240.Google Scholar
  7. 7.
    Mitruka, B. M., Kundargi, R. S. & Jonas, A. M., 1972. Gas chromatography for rapid differentiation of bacterial infections in man. Med. Res. Eng. 11: 7–11.Google Scholar
  8. 8.
    Reiner, E., Hicks, J. J., Ball, M. M. & Martin, W. J., 1972. Rapid characterization of Salmonella organisms by means of pyrolysis gas liquid chromatography. Anal. Chem. 44: 1058–1061.Google Scholar

Copyright information

© Dr W. Junk Publishers 1983

Authors and Affiliations

  • M. Gunasekaran
    • 1
    • 2
  • W. T. Hughes
    • 1
    • 2
  • Richard B. Wilber
    • 1
    • 2
  1. 1.Department of BiologyFisk UniversityNashvilleUSA
  2. 2.Division of Infectious DiseasesSt. Jude Children's Research HospitalMemphisUSA

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