Summary
Analysis of cloned SalI fragments coding for the 26S rRNA in wheat mitochondria (Triticumaestivum L.) shows that the 26S gene is contained within the same basic structural unit RS (which is about 6.6 kbp long). This repeated sequence is flanked by four different sequences I, II, III, IV in the orientations: I-RS-III, I-RS-IV, II-RS-III and II-RS-IV. This organization of the 26S gene, similar to the organization found for the 5S and 18S gene, suggests again that reciprocal intra-and/or intermolecular recombination occurs within these repeated sequences. S1 mapping and R loop mapping indicate that the 26S gene is about 3.5 kbp and has no intron.
Similar content being viewed by others
References
Berk AJ, Sharp PA (1977) Cell 12:721–732
Berkner KL, Folk WR (1977) J Biol Chem 252:3176–3184
Bonen L, Gray MW (1980) Nucleic Acids Res 8:319–335
Chao S, Sederoff RR, Levings CS III (1983) Plant Physiol 71:190–193
Dale RMK, Mendu N, Ginsburg H, Kridl JC (1984) Plasmid 11:141–150
Falconet D, Lejeune B, Quétier F, Gray MW (1984) EMBO J 3:297–302
Hohn B, Collins J (1980) Gene 11:291–298
Huh TY, Gray MW (1982) Plant Mol Biol 1:245–249
Iams KP, Sinclair JH (1982) Proc Natl Acad Sci USA 79:5926–5929
Leaver CJ, Gray MW (1982) Annu Rev Plant Physiol 33:373–402
Lebacq P, Vedel F (1981) Plant Sci Lett 23:1–9
Lonsdale DM, Hodge TP, Fauron CMR, Flavell RB (1983) In: Goldberg RB (ed) UCLA Symposium on Molecular and Cellular Biology 12:445–456
Palmer JD, Shields CR (1984) Nature 307:437–440
Quétier F, Vedel F (1977) Nature 268:365–368
Quétier F, Lejeune B, Delorme S, Falconet D (1984) Encyclopedia of Plant Physiol 18 (in the press)
Sederoff RR (1984) Adv Genet 22:1–108
Seidman JG, Max EE, Leder P (1979) Nature 280:370–375
Spencer DF, Bonen L, Gray MW (1981) Biochemistry 20:4022–4029
Spencer DF, Schnare MM, Gray MW (1984) Proc Natl Acad Sci USA 81:493–497
Southern EM (1975) J Mol Biol 98:503–517
Stern DB, Palmer JD (1984) Nucleic Acids Res 12:6141–6157
Stern DB, Dyer TA, Lonsdale DM (1982) Nucleic Acids Res 10:3333–3340
Wallace DC (1982) Microbiol Rev 46:208–240
Ward BL, Anderson RS, Bendich AJ (1981) Cell 25:793–803
White RL, Hogness DS (1977) Cell 10:177–192
Wu R (1970) J Mol Biol 51:501–521
Zieg J, Silverman M, Hilmen M, Simon M (1977) Science 196:170–172
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Falconet, D., Delorme, S., Lejeune, B. et al. Wheat mitochondrial 26S ribosomal RNA gene has no intron and is present in multiple copies arising by recombination. Curr Genet 9, 169–174 (1985). https://doi.org/10.1007/BF00436966
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00436966