Skip to main content
SpringerLink
Log in

Rapid alkaline preparation for yeast circular covalently closed DNA molecules

  • Original Articles
  • Published:
Current Genetics Aims and scope Submit manuscript

Summary

The alkaline preparation of prokaryotic plasmids (Birnboim and Doly, 1979) has been here adapted to yeast. By simple denaturation and renaturation steps we recovered, from Saccharomyces cerevisiae and Schizosaccharomyces pombe, a population of nucleic acid molecules highly enriched in circular forms. In S. cerevisiae killer strains it is possible to copurify double stranded RNA molecules. The overall recovery was estimated to be 10–30% of the total circular molecules.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ballario P, Filetici P, Junakovic N, Pedone F (1983) FEBS Letters 155:225–229

    Google Scholar 

  • Bevan EA, Herring G (1976) In: Bandlow W, (eds) Genetics, biogenesis and bioenergetics of mitochondria, p 153

  • Bina Stein M, Vogel T, Singer DS, Singer MS (1976) J Biol Chem 251:7363–7366

    Google Scholar 

  • Birnboim HC, Doly J (1979) Nucleic Acid Res 7:1513–1523

    Google Scholar 

  • Botstein D, Davis RW (1982) In: Strathern JN, (eds) The molecular biology of the yeast saccharomyces, part II. Cold Spring Harbor Laboratory, pp 607–636

  • Broach JR (1981) In: Strathern JN, (eds) The molecular biology of the yeast saccharomyces, part I. Cold Spring Harbor Laboratory, pp 445–470

  • Cameron JR, Philippsen P, Davis RW (1977) Nucleic Acids Res 4:1429–1448

    Google Scholar 

  • Clark-Walker GD, Azad AA (1980) Nucleic Acids Res 8:1009–1022

    Google Scholar 

  • Cox BS (1965) Heredity 20:505–521

    Google Scholar 

  • Fournier P, Gaillardin C, de Louvencourt L, Heslot H, Lang BF, Kaudewitz F (1982) Curr Genet 6:31–38

    Google Scholar 

  • Garvick B, Harber JE (1978) J Bacteriol 134:261–269

    Google Scholar 

  • Gerbaud C, Guerineau M (1980) Current Genetics 1:219–228

    Google Scholar 

  • Guerineau M, Grandchamp C, Paoletti C, Slonimsk P (1971) Biochem Biophys Res Commun 42:550–557

    Google Scholar 

  • Harley JL, Donelson JE (1980) Nature 286:860–864

    Google Scholar 

  • Lacroute F (1971) J Bacteriol 106:519–522

    Google Scholar 

  • Larionov UL, Grishilm AV, Parshokva TA, Trangott N (1981) Molekulyarnaya Biologya 15:124–131

    Google Scholar 

  • Sherman F, Fink GR, Hicks J (1981) Methods in yeast genetics. Cold Spring Harbor Laboratory

  • Southern EM (1975) J Mol Biol 98:503–515

    Google Scholar 

  • Wickner RB (1981) In: Strathern J, (eds) The molecular biology of the yeast saccharomyces, part I. Cold Spring Harbor Laboratory, pp 415–444

Download references

Author information

Authors and Affiliations

  1. Centro di Studio per gli Acidi Nucleici, Italy

    Patrizia Filetici & Nikolaj Junakovic

  2. Dipartimento di Genetica e Biologia Moleculare, c/o Istituto di Fisiologia Generale, Città Universitaria, I-00185, Roma, Italy

    Paola Ballario

Authors
  1. Patrizia Filetici
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nikolaj Junakovic
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Paola Ballario
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Filetici, P., Junakovic, N. & Ballario, P. Rapid alkaline preparation for yeast circular covalently closed DNA molecules. Curr Genet 9, 123–126 (1985). https://doi.org/10.1007/BF00436959

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00436959

Key words

Search

Navigation