Cellulolytic enzymes associated with the fruit rots of Citrus sinensis caused by Aspergillus aculeatus and Botryodiplodia theobromae
Botryodiplodia theobromae and Aspergillus aculeatus were inoculated on carboxymethylcellulose (CMC) medium and filter papers. The hydrolysis of the CMC medium and degradation of the filter papers were observed, indicating the production of the Cl and Cx cellulases by the two rot pathogens. The Cl and Cx enzymes were also detected in filtrates of rotted orange fruits incited by the two rot pathogens.
The cellulases could not induce rot development on their own. However, when they were added to pectinases in an enzyme inoculum, the incubation period for inducing rot development was shorter; thus establishing a secondary role for the cellulases in the rot development. This secondary role of the cellulases produced by the two fungi was found to be at peak at pH 7 and a temperature range of 25°–30 °C in the two fungi.
KeywordsEnzyme Hydrolysis Filter Paper Cellulase Aspergillus
Unable to display preview. Download preview PDF.
- Fergus, C. L., 1964. Thermophilic and thermotolerant molds and actinomycetes of mushroom compost during peak heating. Mycologia 56: 267–284.Google Scholar
- Garrett, S. D., 1962. Decomposition of cellulose in soil by Rhizoctonia solani Kuhn. Tran. Brit. mycol. Soc. 45: 115–120.Google Scholar
- Hash, J. H. & King, K. W., 1958. On the nature of the B-Glucosidases of Myrothecium verrucaria. J. biol. Chem. 232: 381–393Google Scholar
- Husain, A., 1958. Production of cellulolytic enzymes by Sclerotium rolfsii. Phytopathology 48: 338–340.Google Scholar
- Husain, A. & Dimond, A. E., 1958. Cellulolytic activity of some apple rotting fungi. Phytopathology 48: 263.Google Scholar
- Kooiman, P., 1957. Some properties of cellulase of Myrothecium verrucaria and some other fungi. II. Enzymologia XVIII: 371–384.Google Scholar
- Mandels, M. & Reese, E. T., 1965. Inhibition of cellulases. A. Rev. Phytopath. 3: 85–102.Google Scholar
- Mandels, M. & Steinberg, D., 1976. Recent advances in cellulase technology. J. Ferment. Technol. Osaka 54: 267–286.Google Scholar
- Nolan, R. A., 1976. Physiological studies on an isolate of Saprolegnia ferax from the larval Gut of the Blackfly Simulium vittatum. Mycologia LXVIII: 523–540.Google Scholar
- Reese, E. T. & Levinson, H. S., 1952. A comparative study of the breakdown of cellulose by microorganisms. Physiologia Pl. 5: 345–366.Google Scholar
- Reese, E. T. & Mandels, M., 1963. Enzymic hydrolysis of cellulose and its derivatives, p. 139–143. In R. L. Whisler (ed.) Methods in carbohydrate chemistry vol. 3 Academic Press N. Y.Google Scholar
- Spalding, D. H., 1963. Production of Pectinolytic and Cellulolytic enzymes by Rhizopus stolonifer. Phytopathology 53: 927–931.Google Scholar
- Srivastava, D. N., Echandi, E. & Walker, J. C., 1959. Pectolytic and Cellulolytic enzymes produced by Rhizopus stolonifer. Phytopathology 49: 145–148.Google Scholar
- Thomas, R. & Whitaker, D. R., 1958. Zone Electrophoresis of Myrothrecium cellulase. Nature Lond. 181: 715–716.Google Scholar
- Tribe, H. T., 1955. Studies in the Physiology of Parasitism. XIX. On the killing of plant cells by enzymes from Botrytis cinerea and Bacterium aroideae. Ann. Bot. XIX: 351–368.Google Scholar
- Winstead, N. W. & Walker, J. C., 1954. Production of vascular browning by metabolites from several fungi. Phytopathology 44: 153–158.Google Scholar
- Wood, R. K. S., 1960. Pectic and cellulolytic enzymes in plant diseases. A. Rev. Pl. Physiol. 11: 299–322.Google Scholar
- Wood, R. K. S., 1971. The killing of plant cells by soft rot parasites. In Phytotoxins in Plant diseases. edit. by Wood, R. S. K., Ballie, A. & Graniti, A. Academic Press London. N.Y.Google Scholar