Mycopathologia

, Volume 106, Issue 1, pp 31–34 | Cite as

Itraconazole vs amphotericin B: in vitro comparative evaluation of the minimal inhibitory concentration (MIC) against clinically isolated yeasts

  • G. Lombardi
  • G. Gramegna
  • C. Cavanna
  • G. Poma
  • E. Marangoni
  • G. Michelone
Article

Abstract

Itraconazole is a triazole compound which, following several clinical trials, has begun to be used for therapy of mycotic infections. This new drug, with a broad-spectrum antifungal activity, can be orally administered. The Authors studied the in vitro susceptibility to amphotericin B and itraconazole of the following clinical isolates of pathogenic yeasts: 100 Candida albicans, 20 C. tropicalis, 20 C. parapsilosis, 8 C. guilliermondii, 6 C. pseudotropicalis, 24 Torulopsis glabrata and 16 Cryptococcus neoformans.

Serial two-fold dilutions, from 100 μg/ml to 0.04 μg/ml, of each drug were prepared in Yeast Nitrogen Base + Glucose 5%, after dissolving the itraconazole in dimethylsulfoxide (DMSO) and amphotericin B in 5% glucose solution. Amphotericin B (MIC90: 3.12 μg/ml) was found to have an average in vitro MIC six-fold lower than itraconazole (MIC90: 25 μg/ml).

Thus, even though itraconazole is active, amphotericin B remains one of the most effective of the antifungal drugs.

Key words

amphotericin B itraconazole susceptibility testing yeast infections 

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Copyright information

© Kluwer Academic Publishers 1989

Authors and Affiliations

  • G. Lombardi
    • 1
  • G. Gramegna
    • 1
  • C. Cavanna
    • 1
  • G. Poma
    • 1
  • E. Marangoni
    • 1
  • G. Michelone
    • 1
  1. 1.Istituto di Clinica delle Malattie InfettiveUniversità degli Studi di Pavia, IRCCS Policlinico S. MatteoPaviaItaly

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