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Current Genetics

, Volume 12, Issue 3, pp 231–233 | Cite as

Transformation of Fulvia fulva, a fungal pathogen of tomato, to hygromycin B resistance

  • R. P. Oliver
  • I. N. Roberts
  • R. Harling
  • L. Kenyon
  • P. J. Punt
  • M. A. Dingemanse
  • C. A. M. J. J. van den Hondel
Short Communications

Summary

A transformation system for the tomato pathogen Fulvia fulva has been developed. Hygromycin B resistant colonies were obtained after treatment of protoplasts with a plasmid containing an E. coli hygromycin B phosphotransferase gene fused to an Aspergillus nidulans promoter. The DNA was stably integrated into the genome. The number and sites of integrations varied among transformants. The demonstration of transformation opens the way for the molecular genetic analysis of the interaction of Fulvia with tomato.

Key words

Tomato leaf mould Cladosporium fulvum Deuteromycete 

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References

  1. Butler EJ, Jones SG (1949) Plant pathology. MacMillan, London, pp 672–678Google Scholar
  2. De Wit PJGM, Spikman G (1982) Physiol Plant Pathol 21:1–11Google Scholar
  3. Gritz L, Davies J (1983) Gene 25:179–188Google Scholar
  4. Harling R, Kenyon L, Lewis BG, Oliver RP, Turner JG, Coddington A (1987) J Phytopathol (in press)Google Scholar
  5. Kaster KR, Burgett SG, Ignolia TD (1984) Curr Genet 8:353–358Google Scholar
  6. Maniatis T, Fritsch EF, Sambrook J ((1982) tMolecular cloning. A laboratory manual. lCold Spring Harbor, New YorkGoogle Scholar
  7. Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, van den Hondel CAMJJ (1987) Gene (in press)Google Scholar
  8. Queener SW, Ignolia TD, Skatrud PL, Chapman JL, Kaster KR (1985) A system for genetic transformation of Cephalosporium. Schlessinger D (ed) Microbiology. Amer Soc Micriobiol, pp 468–471Google Scholar
  9. Raeder U, Broda P (1985) Lett Appl Microbiol 1:17–20Google Scholar
  10. Vollmer SJ, Yanofsky C (1986) Proc Natl Acad Sci USA 83:4869–4873Google Scholar
  11. Yoder OC, Weltring K, Turgeon BC, Garber RC, van Etten HD (1986) Technology for molecular cloning of fungal virulence genes. Nato ASI Series, vol HI: Biology and molecular biology of plant-pathogen interactions. Bailey, pp 371–384Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • R. P. Oliver
    • 1
  • I. N. Roberts
    • 1
  • R. Harling
    • 1
  • L. Kenyon
    • 1
  • P. J. Punt
    • 2
  • M. A. Dingemanse
    • 2
  • C. A. M. J. J. van den Hondel
    • 2
  1. 1.Norwich Molecular Plant Pathology Group, School of Biological SciencesUniversity of East AngliaNorwichGB
  2. 2.Medical Biology Laboratory TNORijswijkThe Netherlands

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