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Studies of the effects of spherulin from Coccidioides immitis on human polymorpho-nuclear leukocytes

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Abstract

The effects of spherule lysate (spherulin) on human polymorphonuclear leukocyte (PMN) function was examined. PMN adherence to glass and capping was increased by spherulin, findings which may account for spherulin's interference with complement-mediated migration through cellulose ester filters. In contrast, PMN attachment to yeast, killing of bacteria, and effects on spherules were virtually unaffected by spherulin, suggesting that it does not directly inhibit PMN antimicrobial function.

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References

  1. Albertini, D. F., Berlin, R. D. & Oliver, J. M., 1977. The mechanism of concanavalin A cap formation in leukocytes. J. Cell Sci. 26: 57–75.

    Google Scholar 

  2. Brass, C., Levine, H. B. & Stevens, D. A., 1982. Stimulation and suppression of cell mediated immunity by endosporuiation antigens of Coccidioides immitis. Infect. Immun. 35: 431–436.

    Google Scholar 

  3. Cohen, I. M., Galgiani, J. N., Potter, D. & Ogden, D. A., 1982. Coccidioidomycosis in renal replacement therapy. Arch. Int. Med. 142: 489–494.

    Google Scholar 

  4. Difco Manual, 9th ed., 1977. Difco, Detroit.

  5. Drutz, D. J. & Catanzaro, A., 1978. Coccidioidomycosis. Part II. Am. Rev. Respir. Dis. 177: 727–771.

    Google Scholar 

  6. Drutz, D. J., Huppert, M., Sun, S. H. & McGuire, W. L., 1981. Human sex hormones stimulate the growth and maturation of Coccidioides immitis. Infect. Immun. 32: 897–907.

    Google Scholar 

  7. Dugger, K. O. & Galgiani, J. N., 1982. Direct effects of activated serum on polymorphonuclear leukocytes as measured by a new microbicidal assay. Am. Rev. Respir. Dis. 125: 175.

    Google Scholar 

  8. Ferrante, A. & Thong, Y. H., 1978. A rapid one-step procedure for purification of mononuclear and polymorphonuclear leukocytes from human blood using a modification of the hypaque-ficoll technique. J. Immunol. Meth. 24: 389–393.

    Google Scholar 

  9. Friedman, L., Smith, C. E., Roessler, W. G. & Berman, R. J., 1956. The virulence and infectivity of twenty-seven strains of Coccidioides immitis. Am. J. Hygiene 64: 198–210.

    Google Scholar 

  10. Gale, D., Lockhart, E. A. & Kimbell, E., 1967. Studies of Coccidioides immitis. III: Further studies of toxic soluble components of Coccidioides immitis. In Ajello, L., ed. Coccidioidomycosis. Tucson, University of Arizona Press, pp 355–372.

    Google Scholar 

  11. Galgiani, J. N., Hayden, R. & Payne, C. M., 1982. Leukocyte effects on the dimorphism of Coccidioides immitis. J. Infect. Dis. 146: 56–63.

    Google Scholar 

  12. Galgiani, J. N., Isenberg, R. A. & Stevens, D. A., 1978. Chemotaxigenic activity of extracts from the mycelial and spherule phases of Coccidioides immitis for human polymorphonuclear leukocytes. Infect. Immun. 21: 862–865.

    Google Scholar 

  13. Galgiani, J. N., Payne, C. M. & Jones, J. F., 1984. Human polymorphonuclear leukocyte inhibition of incorporation of chitin precursors into mycelia of Coccidioides immitis. J. Infect. Dis. 149: in press.

  14. Galgiani, J. N., Yam, P., Petz, L. D., Williams, P. L. & Stevens, D. A., 1980. Complement activation by Coccidioides immitis: in vitro and clinical studies. Infect. Immun. 28: 944–949.

    Google Scholar 

  15. Gallin, J. I., Seligmann, B. E., Cramer, E. B., Schiffmann, E. & Fletcher, M. P., 1982. Effects of vitamin K on human neutrophil function. J. Immunol. 128: 1399–1408.

    Google Scholar 

  16. Huppert, M., Spratt, N. S., Vukovich, K. R., Sun, S. H. & Rice, E. H., 1978. Antigenic analysis of coccidioidin and spherulin determined by two-dimensional immunoelectrophoresis. Infect. Immun. 20: 541–551.

    Google Scholar 

  17. Keller, H. V. & Sorkin, E., 1967. Studies of chemotaxis IX. Migration of rabbit leukocytes through filter membranes. Proceeding of the Society for Experimental Biology and Medicine. 126: 677–680.

    Google Scholar 

  18. Klock, J. C., Boyles, J., Bainton, D. & Stossel, T. P., 1979. Nylon-fiber-induced neutrophil fragmentation. Blood 54: 1216–1229.

    Google Scholar 

  19. Levine, H. B., Gonzalez-Ochoa, A. & Ten Eyck, D. R., 1973. Dermal sensitivity to Coccidioides immitis. A comparison of responses elicited in man by spherulin and coccidioidin. Am. Rev. Respir. Dis. 107: 379–386.

    Google Scholar 

  20. Oliver, J. M., Gelfand, E. W., Pearson, E. B., Pfeiffer, J. R. & Dosch, H.-M., 1980. Microtubule assembly and concanavalin A capping in lymphocytes: Reappraisal using normal and abnormal human peripheral blood cells. Cell Biol. 77: 3499–3503.

    Google Scholar 

  21. Pappagianis, D., 1983. Coccidioidomycosis (San Joaquin or Valley Fever). In DiSalvo, A., ed., Occupational Mycoses. Philadelphia. Lea and Febiger, pp 13–28.

    Google Scholar 

  22. Rodenbiker, H. T. & Ganley, J. P., 1980. Ocular Coccidioidomysis. Surv. Ophthamol. 24: 263–290.

    Google Scholar 

  23. Root, R. K., Rosenthal, A. S. & Balestra, D. J., 1972. Abnormal bactericidal, metabolic, and lysosomal functions of Chediak-Higashi syndrome leukocytes. J. Clin. Invest. 51: 649–665.

    Google Scholar 

  24. Scalarone, G. M., Levine, H. B., Chaparas, S. D. & Cobb, J. M., 1973. Properties and assay of spherulins from Coccidioides immitis on delayed sensitivity responses of animals. Sabouraudia 11: 222–234.

    Google Scholar 

  25. Scharmann, W., Jacob, F. & Porstendorfer, J., 1976. The cytotoxic action of leucocidin from Pseudomonas aeruginosa on human polymorphonuclear leukocytes. J. Gen. Micro. 93: 303–308.

    Google Scholar 

  26. Shurin, S. B., Socransky, S. S., Sweeny, E. & Stossel, T. P., 1979. A neutrophil disorder induced by capnocytophagia, a dental micro-organism. New Engl. J. Med. 301: 849–854.

    Google Scholar 

  27. Smith, C. E., 1940. Epidemiology of acute coccidioidomycosis with erythema nodosum (‘San Joaquin’ or ‘Valley Fever’). Am. J. Public Health 30: 600–611.

    Google Scholar 

  28. Wallraff, E. B. & VanLiew, R. M., 1967. Rabbit alveolar macrophage response to in vitro addition of coccidioidin. In Ajello, L., ed. Coccidioidomycosis. Tucson, University of Arizona Press. pp 179–181.

    Google Scholar 

  29. Werner, S. B., Pappagianis, D., Heindl, I. & Michael, I., 1972. An epidemic of Coccidioidomycosis among archeology students in Northern California. N. Engl. J. Med. 286: 507–512.

    Google Scholar 

  30. Wilkinson, P. C., 1974. Chemotaxis and inflammation. London, Churchill Livingston.

    Google Scholar 

  31. Wright, D. G., Kauffmann, J. C., Chusid, M. J., Herzig, G. P. & Gallin, J. I., 1975. Functional abnormalities of human neutrophils collected by continuous flow filtration leukopherisis. Blood 46: 901–911.

    Google Scholar 

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  1. The Medical and Research Services, Veterans Administration Medical Center and the Department of Internal Medicine, University of Arizona College of Medicine, 85723, Tucson, AZ, USA

    John N. Galgiani

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Galgiani, J.N. Studies of the effects of spherulin from Coccidioides immitis on human polymorpho-nuclear leukocytes. Mycopathologia 90, 107–111 (1985). https://doi.org/10.1007/BF00436860

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