, Volume 115, Issue 2, pp 89–103 | Cite as

An integrated approach to Phoma systematics

  • E. Montel
  • P. D. Bridge
  • B. C. Sutton


Physiological and morphological characters were recorded from 55 strains of 17 Phoma taxa and one Pyrenochaeta. The results were subjected to numerical analysis and UPGMA dendrograms produced. The full results were compared with TLC profiles of secondary metabolites. Seven distinct clusters were recovered from dendrograms based on full and partial character sets and the grouping of strains within each cluster discussed. The new combination Phoma sambuci-nigrae (Sacc.) Monte, Bridge & Sutton is proposed for P. herbarum f. sambuci-nigrae Sacc.

Key words

Phoma systematics physiology secondary metabolites numerical analysis 


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  1. 1.
    Boerema GH. Phoma herbarum Westend., the type-species of the form-genus Phoma Sacc. Persoonia 1963; 3: 9–16.Google Scholar
  2. 2.
    Boerema GH. The Phoma organisms causing gangrene of potatoes. Neth J Plant Pathol 1967; 73: 190–192.Google Scholar
  3. 3.
    Boerema GH. The Phoma species studied in culture by Dr RWG Dennis. Trans Brit Mycol Soc 1976; 67: 289–319.Google Scholar
  4. 4.
    Boerema GH, Dorenbosch MMJ. The Phoma and Ascochyta species described by Wollenweber and Hochapfel in their study on fruit-rotting. Stud Mycol 1973; 3: 1–50.Google Scholar
  5. 5.
    Boerema GH, Dorenbosch MMJ, Van Kesteren HA. Remarks on species of Phoma referred to Peyronellaea, V Kew Bull 1977; 31: 533–544.Google Scholar
  6. 6.
    Boerema GH, Höweler LH. Phoma exigua Desm. and its varieties. Persoonia 1967; 5: 15–28.Google Scholar
  7. 7.
    Boerema GH, Van Kesteren HA. Nomenclatural notes on some species of Phoma sect. Plenodomus. Persoonia 1981; 11: 317–331.Google Scholar
  8. 8.
    Booth C. The Genus Fusarium. Kew, UK: CAB International Mycological Institute. 1971.Google Scholar
  9. 9.
    Bridge PD. An evaluation of some physiological and biochemical methods as an aid to the characterization of species of Penicillium subsection Fasciculata. J Gen Microbiol 1985; 131: 1887–1895.Google Scholar
  10. 10.
    Bridge PD, Hawksworth DL, Kozakiewicz Z, Onions AHS, Paterson RRM & Sackin MJ. An integrated approach to Penicillium systematics. In Advances in Penicillium and Aspergillus Systematics (ed. RA Samson & JI Pitt), 1986; 281–309.Google Scholar
  11. 11.
    Bridge PD, Hawksworth DL, Kozakiewicz Z, Onions AHS, Paterson RRM, Sackin MJ & Sneath PHA. A reappraisal of the Terverticillate Penicillia using biochemical physiological and morphological features. I Numerical taxonomy. J Gen Microbiol 1989; 135: 2941–2966.Google Scholar
  12. 12.
    Bridge PD, Sackin MJ. Stability of classification of filamentous fungi under changes in character coding strategy. Mycopathologia 1991; 115: 105–111.Google Scholar
  13. 13.
    Dabinett PE, Wellman AM. Numerical taxonomy of certain genera of Fungi Imperfecti and Ascomycotina. Can J Bot 1978; 56: 2031–2049.Google Scholar
  14. 14.
    Davis ND, Diener UL, Eldridge DW. Production of aflatoxins B1 and G1 by Aspergillus flavus in a semisynthetic medium. Appl Microbiol 1966; 14: 378–380.Google Scholar
  15. 15.
    Dennis RWG. Notes on some British fungi ascribed to Phoma and related genera. Trans Brit Mycol Soc 1946; 29: 11–42.Google Scholar
  16. 16.
    Dorenbosch MMJ. Key to nine ubiquitous soil-borne Phoma-like fungi. Persoonia 1970; 6: 1–14.Google Scholar
  17. 17.
    Frisvad JC. Physiological criteria and mycotoxin production as aids in identification of common asymmetric penicillia. Appl Envir Microbiol 1981; 41: 568–579.Google Scholar
  18. 18.
    Kidd CBM, Caddy B, Robertson J, Tebbett IR, Watling R. Thin-layer chromatography as an aid for identification of Dermocybe species of Cortinarius. Trans Brit Mycol Soc 1985; 85: 213–221.Google Scholar
  19. 19.
    Logan C, Khan AA. Comparative studies of Phoma spp. associated with potato gangrene in northern Ireland. Trans Brit Mycol Soc 1969; 52: 9–17.Google Scholar
  20. 20.
    Logan C, O'Neill R. Production of an antibiotic by Phoma exigua. Trans Brit Mycol Soc 1970; 55: 67–75.Google Scholar
  21. 21.
    Lynch JM, Slater JH, Bennett JA, Harper SHT. Cellulase activities of some aerobic micro-organisms isolated from soil. J Gen Microbiol 1981; 127: 231–236.Google Scholar
  22. 22.
    Morgan-Jones G. Studies in the genus Phoma. XIV. Concerning Phoma herbarum, the type species, a widespread saprophyte. Mycotaxon 1988; 33: 81–90.Google Scholar
  23. 23.
    Morgan-Jones G, Burch KB. Studies in the genus Phoma. VIII. Concerning Phoma medicaginis var. medicaginis. Mycotaxon 1987; 29: 477–487.Google Scholar
  24. 24.
    Morgan-Jones G, Burch KB. Studies in the genus Phoma. IX. Concerning Phoma jolyana. Mycotaxon 1987; 30: 239–246.Google Scholar
  25. 25.
    Morgan-Jones G, Burch KB. Studies on the genus Phoma. X. Concerning Phoma eupyrena, an ubiquitous, soil-borne species. Mycotaxon 1988; 31: 427–434.Google Scholar
  26. 26.
    Morgan-Jones G, White JF. Studies in the genus Phoma. I. Phoma americana sp. nov. Mycotaxon 1983; 16: 403–413.Google Scholar
  27. 27.
    Mugnai L, Bridge PD, Evans HC. A chemosystematic evaluation of the genus Beauveria. Mycol Res 1989; 92: 199–209.Google Scholar
  28. 28.
    Paterson RRM. Standardized one and two-dimensional thin-layer chromatographic methods for the identification of secondary metabolites in Penicillium and other fungi. J Chromatogr 1986; 368: 249–264.Google Scholar
  29. 29.
    Pitt JI. The Genus Penicillium and its Teleomorphic States Eupenicillium and Talaromyces. London: Academic Press. 1980.Google Scholar
  30. 30.
    Punithalingam E. Sphaeropsidales in culture from humans. Nova Hedwigia 1979; 31: 119–158.Google Scholar
  31. 31.
    Raper KB & Fennell DL. The Genus Aspergillus. Baltimore: Williams & Wilkins. 1965.Google Scholar
  32. 32.
    Rayner RW. A Mycological Colour Chart. Kew: CAB International Mycological Institute & British Mycological Society. 1970.Google Scholar
  33. 33.
    Sackin MJ. Comparisons of classifications. In Computer assisted bacterial Systematics (ed. M Goodfellow, D Jones & FG Priest), pp. 21–36. London: Academic Press. 1985.Google Scholar
  34. 34.
    Schneider R. Die Gattung Pyrenochaeta De Notaris. Mitteilung aus der Biologischen Bundesanstalt für Land- und Forstwirtschaft Berlin-Dahlem 1979; 189: 1–73.Google Scholar
  35. 35.
    Smith D, Onions AHS. The Preservation and Maintenance of Living Fungi. Kew: CAB International Mycological Institute. 1983.Google Scholar
  36. 36.
    Sneath PHA, Sokal RR. Numerical Taxonomy. San Francisco: WH Freeman. 1973.Google Scholar
  37. 37.
    Sutton BC. Coelomycetes VI. Nomenclature of generic names proposed for Coelomycetes. Mycol Pap (IMI) 1977; 141: 1–253.Google Scholar
  38. 38.
    Sutton BC. The Coelomycetes. Kew, UK: CAB International Mycological Institute. 1980.Google Scholar
  39. 39.
    White JF, Morgan-Jones G. Studies on the genus Phoma. VI. Concerning Phoma medicaginis var. pinodella. Mycotaxon 1987a; 28: 241–248.Google Scholar
  40. 40.
    White JF, Morgan-Jones G. Studies on the genus Phoma. VII. Concerning Phoma glomerata. Mycotaxon 1987b; 28: 437–445.Google Scholar
  41. 41.
    Wollenweber HW, Hochapfel H. Beiträge zur Kenntnis parasitärer und saprophytischer Pilze. I. Phomopsis, Dendrophoma, Phoma und Ascochyta und ihre Beziehung zur Fruchtfäule. Zeitschr Parasit 1936; 8: 561–605.Google Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • E. Montel
    • 1
  • P. D. Bridge
    • 2
  • B. C. Sutton
    • 2
  1. 1.Departamento de Microbiología, Genética, Medicina, Preventiva y Salud PúblicaUniversidad de SalamancaSalamancaSpain
  2. 2.International Mycological InstituteKewUK

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