Mycopathologia

, Volume 112, Issue 3, pp 127–137 | Cite as

Clinical and experimental mycotic keratitis caused by Aspergillus terreus and the effect of subconjunctival oxiconazole treatment in the animal model

  • S. M. Singh
  • Sapna Sharma
  • P. K. Chatterjee
Article

Abstract

Aspergillus terreus was isolated from a case of Keratomycosis. The patient, a 50 year old, female presented with a large corneal ulcer with hypopyon. The direct microscopic examination of the scrapings revealed hyaline, thin, septate and branched hyphae. In vitro some antimycotics (amphotericin B,5-fluorocytosine, oxiconazole, amorolfine and ketoconazole) were tested against A. terreus by agar dilution method. Ketoconazole with MIC of 3 gmg/ml after 7 days of incubation was most effective followed by oxiconazole (10μg/ml). Experimental corneal ulcer was produced by injecting intralamellary 0.1 ml of the spore suspension containing 10×106 cfu/ml into the eyes of previously immunocompressed albino rabbits. Histopathologic examination showed infiltration and large destruction of the corneal stroma. Subconjunctival oxiconazole therapy exhibited complete cure. Based on our findings, a clinical evaluation of oxiconazole in human keratomycosis seems to be justified.

Key words

Keratomycosis aspergillus oxiconazole 

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Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • S. M. Singh
    • 1
  • Sapna Sharma
    • 1
  • P. K. Chatterjee
    • 1
    • 2
  1. 1.Department of Biological SciencesRani Durgavati UniversityJabalpurIndia
  2. 2.JabalpurIndia

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