, Volume 112, Issue 3, pp 127–137 | Cite as

Clinical and experimental mycotic keratitis caused by Aspergillus terreus and the effect of subconjunctival oxiconazole treatment in the animal model

  • S. M. Singh
  • Sapna Sharma
  • P. K. Chatterjee


Aspergillus terreus was isolated from a case of Keratomycosis. The patient, a 50 year old, female presented with a large corneal ulcer with hypopyon. The direct microscopic examination of the scrapings revealed hyaline, thin, septate and branched hyphae. In vitro some antimycotics (amphotericin B,5-fluorocytosine, oxiconazole, amorolfine and ketoconazole) were tested against A. terreus by agar dilution method. Ketoconazole with MIC of 3 gmg/ml after 7 days of incubation was most effective followed by oxiconazole (10μg/ml). Experimental corneal ulcer was produced by injecting intralamellary 0.1 ml of the spore suspension containing 10×106 cfu/ml into the eyes of previously immunocompressed albino rabbits. Histopathologic examination showed infiltration and large destruction of the corneal stroma. Subconjunctival oxiconazole therapy exhibited complete cure. Based on our findings, a clinical evaluation of oxiconazole in human keratomycosis seems to be justified.

Key words

Keratomycosis aspergillus oxiconazole 


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  1. 1.
    Balakrishnan, E. Mycotic Keratitis Caused by Aspergillus fumigatus. Brit J Ophthalmol 1961; 45: 828.Google Scholar
  2. 2.
    Borelli D, Rodriguez H & Marceno C. Petriasis Versicolor: Tratamiento Per os Con Ketoconazole 1979 III: 19–23.Google Scholar
  3. 3.
    De Voe AC & Silva Hutner M. Fungal infections of the eye, p. 208–240. In D Locatcher-Khorazo & BC Siegel (ed) Microbiology of the Eye. CV Mosby, St. Louis, MO.Google Scholar
  4. 4.
    Fons A, Garcia de Lomas J, Nagueira JM, Busesa FJ & Gimeno C. Histopathology of Experimental Aspergillus fumigatus keratitis. Mycopathol 1988; 129–131.Google Scholar
  5. 5.
    Foster CS, Lass JH, Moran Wallace K & Giovanoni R. Ocular toxicity of topical antifungal agents. Arch Ophthalmol 1981; 99: 1081.Google Scholar
  6. 6.
    Garcia de Lomas J, Fons MA, Nogueira JM, Rustom F, Borras R & Buesa FJ. Mycopathol 1985; 89: 135–138.Google Scholar
  7. 7.
    Ishibashi Y & Matsumoto Y. Intravenous miconazaletherapy for experimental keratomycosis in rabbits: Sabouraudia: J Med Vet Mycol 1985; 23: 55–61.Google Scholar
  8. 8.
    Jones BR. Principles in the Management of Oculomycosis 31, Edward Jackson Memorial Lecture. Amer J Ophthalmol 1975; 79: 719–751.Google Scholar
  9. 9.
    Madan Mohan, Facs MS, Mams JLF, Sangave MD and Mahajan VM. Pathogenesis of Experimentally produced Corneal Ulcer in rabbits. Ann Ophthal 1984; 16(3): 246–251.Google Scholar
  10. 10.
    Naumann G, Green WR & Zimmerman LE. Mycotic Keratitis: A histopathology study of 73 cases. Amer J Ophthalmol 1967; 64: 668–681.Google Scholar
  11. 11.
    Polak A. Antifungal activity in vitro of Ro14-4767, A phenyl propyl morpholine. Sabouraudia 1983; 21: 205–213.Google Scholar
  12. 12.
    Polak A. Oxiconazole, a new imidazole derivative. Drug Res 1982; 32: 17–24.Google Scholar
  13. 13.
    Puttana ST. Primary Keratomycosis. J All India Ophthalmol Soc 1969; 17: 171.Google Scholar
  14. 14.
    Sandhu DK & Rattan AS. Keratomycosis — A Review. Mykosen 1980; 24(8): 503–515.Google Scholar
  15. 15.
    Shukla PK, Khan ZA, Lal B, Agarwal PK & Shrivastava OP. Clinical and Experimental Keratitis caused by Colletotrichum state of Cingulata and Acrophialophora fusispora. Sabouraudia 1983; 21: 137–147.Google Scholar
  16. 16.
    Singh SM, Khan Rubina, Sharma Sapna, Chatterjee, PK. Clinical and Experimental Mycotic Corneal Ulcer Caused by Aspergillus fumigatus and the effect of Oral Ketoconazole in the treatment. Mycopathol 1989 (communicated).Google Scholar
  17. 17.
    Siva-Reddy P, Satyendran OM, Satpathy M, Vijaykumar & Ranga Reddy P. Mycotic Keratitis. Ind J. Ophthalmol 1972; 20: 101.Google Scholar
  18. 18.
    Thomas PA, Rajasekaran J. Treatment of Aspergillus keratitis with Imidazole and related compound: in Aspergillus and Aspergillosis Edited by Hugo Vanden Bossche, DWR Machenzie and Geert Cauwenbergh, Plenum Publishing Corporation. 1988: p. 267–279.Google Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • S. M. Singh
    • 1
  • Sapna Sharma
    • 1
  • P. K. Chatterjee
    • 1
    • 2
  1. 1.Department of Biological SciencesRani Durgavati UniversityJabalpurIndia
  2. 2.JabalpurIndia

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