Abstract
The action of allylamine antifungal agents on Epidermophyton floccosum was studied using scanning electron microscopy. After 7 days of culture on Sabouraud dextrose agar, Epidermophyton floccosum samples were brought in contact with concentrations of 0.2 and 2 μg ml−1 and 0.01 and 0.1 μg ml−1 of naftifine and terbinafine, respectively. Lesions observed after 24 h, 3 and 7 days of contact were mainly on the structure and rigidity of the mycelial and macroconidial wall. They were characterized by hyphal ballooning and twisting and by apical bulbous bulges. Deterioration of macroconidia was characterized by wall exfoliation. The intensity of the deterioration depended on the dose and only slightly on the length of time that the sample and the antifungal drug were in contact.
Résumé
L'action des allylamines sur Epidermophyton floccosum est étudiée en microscopie électronique à balayage. Après 7 jours de culture sur le milieu gélosé de Sabouraud, les échantillons d'Epidermophyton floccosum sont mis en contact avec des concentrations de 0,2 et 2 μg ml−1 et 0,01 et 0,1 μg ml−1 de naftifine et de terbinafine respectivement. Les lésions observées après 24 h, 3 et 7 jours de contact portent principalement sur des modifications de l'aspect morphologique des filaments mycéliens et des macroconidies. Elles sont caractérisées sur le mycélium par des ballonnements, des torsions et des apex bulbiformes. Sur les macroconidies on observe une exfoliation importante de leur paroi. L'intensité de ces lésions est dépendante de la dose et seulement faiblement dépendante du temps de contact entre l'échantillon et l'antifongique.
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Butty, P., Mallié, M. & Bastide, J.M. Antifungal activity of allylamines on Epidermophyton floccosum: scanning electron microscopy study. Mycopathologia 120, 147–153 (1992). https://doi.org/10.1007/BF00436392
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DOI: https://doi.org/10.1007/BF00436392