Abstract
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1.
The present paper deals with the chemolithotrophic growth of a Gram-positive hydrogen bacterium strain 11/x which shows the characteristic features of some coryneform bacteria.
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2.
Like other hydrogen bacteria, the strain 11/x is a facultative chemolithotroph and grows on many organic substrates faster than in a mineral medium under an atmosphere of knallgas+CO2. Fully induced, autotrophically grown cells, subcultured mixotrophically on fructose show additive growth.
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3.
Cell-free extracts of autotrophically grown cells are able to reduce methylene blue, dichlorophenolindophenol, phenazine methosulphate, menadione, and FMN with hydrogen. Conditions for direct NAD(P) reduction could not be found.
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4.
Hydrogenase is formed under autotrophic as well as mixotrophic conditions. In the latter case the rate of hydrogenase formation is diminished depending on the organic substrate. Heterotrophically grown cells do not have any detectable hydrogenase activity. For the induction of hydrogenase in those cells a nitrogen source is a prerequisite.
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5.
The formation of ribulose-1,5-diphosphate carboxylase and phosphoribulokinase seems to be regulated in a way similar to that of hydrogenase: the enzymes could only be detected in autotrophically and mixotrophically grown cells but not in those grown heterotrophically.
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Abbreviations
- Meb:
-
methylene blue
- PMS:
-
phenazine methosulfate
- DCPIP:
-
2,6-dichlorophenolindophenol
- RUDPC:
-
ribulose-1,5-diphosphate carboxylase
- PRK:
-
phosphoribulokinase
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Canevascini, G., Eberhardt, U. Chemolithotrophic growth and regulation of hydrogenase formation in the coryneform hydrogen bacterium strain 11/x . Arch. Microbiol. 103, 283–291 (1975). https://doi.org/10.1007/BF00436362
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DOI: https://doi.org/10.1007/BF00436362