Abstract
Basically the peptidoglycan of Myxobater AL-1 consists of alternating β-1,4-linked N-acetylglucosamic-N-acetylmuramic acid chains. After splitting the aminosugar backbone with a specific algal enzyme three subunits arise: a monomer, a dimer and a trimer. Investigation of the monomer with specific enzymes and comparison of the degradation products to standards derived from other bacterial peptidoglycans suggest the following structure of the monomer peptide: l-alanyl-d-glutamic-l-meso-diaminopimelic-d-alanine. A d-alanyl-d-meso-diaminopimelic acid bond is the bridgebond between the peptides of the subunits.
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Abbreviations
- P1 up to P4:
-
TLC-plates or-papers
- R1 and R2:
-
reagents for localisation
- S1 up to S8:
-
chromatography solvents
References
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Harcke, E., Massow, F.v. & Kühlwein, H. On the structure of the peptidoglycan of cell walls from Myxobacter AL-1 (Myxobacterales). Arch. Microbiol. 103, 251–257 (1975). https://doi.org/10.1007/BF00436358
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DOI: https://doi.org/10.1007/BF00436358