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Changes in phosphatase activity associated with cell wall defects in Chlamydomonas reinhardi

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Abstract

Experiments were performed to isolate mutants lacking alkaline phosphatase in Chlamydomonas reinhardi. Mutants with null enzyme activity were obtained. A cytological study of these mutants however revealed cell wall defects, suggesting that the loss of phosphatase activity in these strains is not due to the inactivation of the corresponding phosphatase structural gene but rather to the leakage of this enzyme as a consequence of the cell wall abnormality. Incidentally, this finding provides the basis of a convenient method for selecting easily cell wall mutants of Chlamydomonas.

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Authors and Affiliations

  1. Laboratoire de Génétique moléculaire, Département de Botanique, Université de Liège, Liège

    R. Loppes & R. Deltour

  2. Laboratoire de Morphologie, végétale, Département de Botanique, Université de Liège, Liège

    R. Loppes & R. Deltour

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  1. R. Loppes
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  2. R. Deltour
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Chercheur qualifié du Fonds National Belge de la Recherche Scientifique.

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Loppes, R., Deltour, R. Changes in phosphatase activity associated with cell wall defects in Chlamydomonas reinhardi . Arch. Microbiol. 103, 247–250 (1975). https://doi.org/10.1007/BF00436357

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  • DOI: https://doi.org/10.1007/BF00436357

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