Abstract
High yields of protoplasts from the 18-hr old mycelium of Trichoderma viride were obtained by using the lytic system, produced by Streptomyces venezuelae RA and Micromonospora chalcea grown on a synthetic medium containing laminarin and chitin, when 0.7 M MgSO4 or (NH4)2SO4 were used as osmotic stabilizers. Regeneration of these protoplasts occurred through the production of an abortive tube and direct germination of the protoplasts. Regeneration could also take place in the medium used to produce protoplasts, but the process was different in many details.
This is a preview of subscription content, log in via an institution to check access.
References
Cabib, E., Bowers, B.: Localization of chitin in yeast bud scars. J. biol. Chem. 246, 152–159 (1971)
Cabib, E., Keller, F.: Allosteric inhibition of chitin-synthetase by a heat-stable protein from yeast. J. biol. Chem. 246, 167–173 (1971)
Gacto, M.: Separación y caracterización de actividades B-(1-3)glucanasas de Micromonospora chalcea. Ph. D. Thesis, University of Salamanca (1973)
García Acha, I., López Belmonte, F., Villanueva, J. R.: Regeneration of mycelial protoplast of Fusarium culmorum. J. gen. Microbiol. 45, 515–523 (1966a)
García Acha, I., Rodríguez Aguirre, M., López Belmonte, F., Villanueva, J. R.: The use of lipase and strepzyme for the isolation of protoplasts from fungal mycelium. Nature (Lond.) 109, 95–96 (1966b)
García Mendoza, C., Novaes-Ledieu, M.: Chitin in the new wall of regenerating protoplasts of Candida utilis. Nature (Lond.) 220, 1035–1048 (1968)
Keller, A., Cabib, E.: Properties of chitin synthetase from Saccharomyces carlsbergensis. J. biol. Chem. 246, 160–166 (1971)
McMurrough, I., Flores Carreon, A., Bartnicki-García, S.: Pathway of chitin synthesis and cellular localization of chitin synthetase in Mucor rouxii. J. biol. Chem. 246, 3999–4007 (1971)
Necas, O.: Cell wall synthesis in yeast protoplasts. Bact. Rev. 35, 149–170 (1971)
Necas, O., Kopecka, M., Brichta, J.: Interpretation of surface structure in frozen-etched protoplasts of yeasts. Exp. Cell Res. 58, 411–419 (1969)
Ramos, S., García Acha, I.: Production and regeneration of protoplasts from Pullularia pullulans. Proceedings of the Third International Symposium on Yeast Protoplasts, Salamanca (1973)
Sierra, J. M.: Estudios sobre lisis enzimática y biosintesis de la pared cellular de levaduras. Ph. D. Thesis, University of Salamanca (1973)
Strunk, C.: Protoplasts of moulds—the present state of knowledge. In: Yeast protoplasts, Acta Facultatis medicae Universitatis Brunensis, O. Necas, A. Svoboda, eds., Vol. 37, pp. 107–119. Brno: University J. E. Purkyne Press 1970
Villanueva, J. R., García Acha, I.: Production and use of fungal protoplasts. In: Methods in microbiology, C. Booth, ed., Vol. 4, pp. 665–718. New York: Academic Press 1971
Villanueva, J. R., García Acha, I.: The phenomenon of regeneration in fungal protoplasts. Rev. lat.-amer. Microbiol. 14, 49–64 (1972)
de Vries, O. M. H., Wessels, J. G. H.: Release of protoplasts from Schizophyllum commune by a lytic enzyme preparation from Trichoderma viride. J. gen. Microbiol. 73, 13–22 (1972)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Benitez, T., Ramos, S. & Garcia Acha, I. Protoplasts of Trichoderma viride . Arch. Microbiol. 103, 199–203 (1975). https://doi.org/10.1007/BF00436350
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00436350