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Histochemical and biochemical evidence for the presence of microbodies in Phytophthora palmivora

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Abstract

Using the diaminobenzidine (DAB) reaction catalase activity could be demonstrated histochemically in cytoplasmic structures of Phytophthora palmivora bearing general ultrastructural features of microbodies. These socalled U-bodies sediment together with the catalase activity in Ficoll-Sorbitol-Sucrose gradients following prior purification by differential centrifugation.

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References

  • Bauer, H., Tanaka, K.: Ultrastructure of mitochondria and crystal containing bodies in mature ballistospores of the fungus Basidiobolus ranarum as revealed by freeze-etching. J. Bact. 96, 2132–2137 (1968)

    Google Scholar 

  • Fahimi, H. D.: Cytochemical localization of peroxidase activity in rat hepatic microbodies (peroxisomes). J. Histochem. Cytochem. 16, 547–550 (1968)

    Google Scholar 

  • Fahimi, H. D.: Cytochemical localization of peroxidatic activity of catalase in rat hepatic microbodies (peroxisomes). J. Cell Biol. 43, 275–288 (1969)

    Google Scholar 

  • Hemmes, D. E., Hohl, H. R.: Ultrastructural changes in directly germinating sporangia of Phytophthora parasitica. Amer. J. Bot. 56, 300–313 (1969)

    Google Scholar 

  • Hemmes, D. E., Hohl, H. R.: Ultrastructural aspects of encystation and cyst-germination in Phytophthora parasitica. J. Cell Sci. 9, 175–192 (1971)

    Google Scholar 

  • Hoffmann, H. P., Szabo, A., Avers, C. J.: Cytochemical localization of catalase activity in yeast peroxisomes. J. Bact. 104, 581–584 (1970)

    Google Scholar 

  • Hohl, H. R., Hamamoto, S. T.: Ultrastructural changes during zoospore formation in Phytophthora parasitica. Amer. J. Bot. 54, 1131–1139 (1967)

    Google Scholar 

  • Hruban, Z., Rechcigl, M.: Microbodies and related particles. Morphology, biochemistry and physiology. New York-London: academic Press 1969

    Google Scholar 

  • Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the folin phenol reagent. J. biol. Chem. 193, 265–275 (1951)

    Google Scholar 

  • Lück, H.: Catalase. In: Methods of enzymatic analyses, H. U. Bergmeyer, ed., pp. 885–888. New York-London: Academic Press 1965

    Google Scholar 

  • Maxwell, D. P., Williams, P. H., Maxwell, M. D.: Microbodies and lipid bodies in the hyphal tips of Sclerotinia sclerotiorum. Canad. J. Bot. 48, 1689–1691 (1970)

    Google Scholar 

  • Mendgen, K.: Microbodies (Glyoxysomes) in infection structures of Uromyces phaseoli. Protoplasma (Wien) 78, 477–482 (1973)

    Google Scholar 

  • Miller, P. M.: V-8 juice agar as a general purpose medium for fungi and bacteria. Phytopathology 30, 461–462 (1955)

    Google Scholar 

  • Parish, R. W.: The influence of Ficoll and Sorbitol on the separation of plant cell organelles. Vjschr. Naturforsch. Ges. Zürich 116, 347–354 (1971)

    Google Scholar 

  • Parish, R. W.: The isolation of peroxisomes, mitochondria and chloroplasts from leaves of spinach beet (Beta vulgaris L. ssp. vulgaris). Europ. J. Biochem. 22, 423–429 (1971)

    Google Scholar 

  • Parish, R. W.: Peroxisomes from the Arum italicum appendix. Z. Pflanzenphysiol. 67, 430–442 (1972)

    Google Scholar 

  • Pitt, D.: Cytochemical evidence for the existence of peroxisomes in Botrytis cinerea. J. Histochem. Cytochem. 17, 613–616 (1969)

    Google Scholar 

  • Reichle, R. E.: Fine structure of Phytophthora parasitica zoospores. Mycologia (N. Y.) 61, 30–51 (1969)

    Google Scholar 

  • Reiss, J.: Cytochemische Darstellung von Peroxisomen in Pilzzellen. Protoplasma (Wien) 72, 43–48 (1971)

    Google Scholar 

  • Roels, F.: Cytochrome c and cytochrome oxidase in diaminobenzidine staining of mitochondria. J. Histochem. Cytochem. 22, 442–446 (1974)

    Google Scholar 

  • Todd, M. M., Vigil, E. L.: Cytochemical localization of peroxidase activity in Saccharomyces cerevisiae. J. Histochem. Cytochem. 20, 344–349 (1972)

    Google Scholar 

  • Wergin, W. P.: Ultrastructural comparison of microbodies in pathogenic and saprophytic hyphae of Fusarium oxysporum f. sp. lycopersici. Phytopathology 62, 1045–1051 (1972)

    Google Scholar 

  • Williams, W. T., Webster, R. K.: Electron microscopy of the sporangium of Phytophthora capsici. Canad. J. Bot. 48, 221–227 (1970)

    Google Scholar 

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Philippi, M.L., Parish, R.W. & Hohl, H.R. Histochemical and biochemical evidence for the presence of microbodies in Phytophthora palmivora . Arch. Microbiol. 103, 127–132 (1975). https://doi.org/10.1007/BF00436339

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  • DOI: https://doi.org/10.1007/BF00436339

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