Abstract
The pattern of ribosomal proteins in plastids of a nonmendelian streptomycin resistant mutant does not differ from that of wildtype cells when compared by two-dimensional gel-electrophoresis. The protein pattern of a mendelian resistance mutant is changed, however.
Since the resistance in this mendelian mutant is probably caused by a change in the ribosomal proteins, the resistance in the nonmendelian mutant must be considered as a change of the ribosomal RNA.
This is a preview of subscription content, log in via an institution to check access.
References
Chua, N.H., Blobel, G., Siekevitz, P.: Isolation of cytoplasmic and chloroplast ribosomes and their dissociation into active subunits from Chlamydomonas reinhardii. J. Cell Biol. 57, 798–814 (1973)
Funatsu, G., Nierhaus, K., Wittmann, H.G.: Ribosomal proteins. XXXVII. Determination of allele types and amino-acid exchanges in protein S 12 of three streptomycin-resistant mutants of Escherichia coli. Biochim. biophys. Acta (Amst.) 287, 282–291 (1972)
Funatsu, G., Wittmann, H.G.: Ribosomal proteins. XXXIII. Location of amino-acid replacements in protein S 12 isolated from Escherichia coli mutants resistant to streptomycin. J. molec. Biol. 68, 547–550 (1972)
Grivell, L.A., Netter, P., Borst, P., Slonimski, P.P.: Mitochondrial antibiotic resistance in yeast: Ribosomal mutants resistant to chloramphenicol, erythromycin and spiramycin. Biochim. biophys. Acta (Amst.) 312, 358–367 (1973)
Hanson, M.R., Davidson, J.N., Mets, L.J., Bogorad, L.: Characterization of chloroplast and cytoplasmic ribosomal proteins of Chlamydomonas reinhardi by two-dimensional gel electrophoresis. Molec. Gen. Genet. 132, 105–118 (1974)
Hoober, J.K., Blobel, G.: Characterization of the chloroplastic and cytoplasmic ribosomes of Chlamydomonas reinhardi. J. molec. Biol. 41, 121–138 (1969)
Kaltschmidt, E., Wittmann, H.G.: Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins. Analyt. Biochem. 36, 401–412 (1970)
Kaltschmidt, E., Wittmann, H.G.: Ribosomal proteins. XXXII. Comparison of several extraction methods for proteins from Escherichia coli ribosomes. Biochimie 54, 167–175 (1972)
Lai, Ch., Dahlberg, J.E., Weisblum, B.: Structure of an inducibly methylatable nucleotide sequence in 23 S ribosomal ribonucleic acid from erythromycin-resistant Staphylococcus aureus. Biochemistry 12, 457–460 (1973a)
Lai, Ch., Weisblum, B., Fahnestock, S.R., Nomura, M.: Alteration of 23 S ribosomal RNA and erythromycininduced resistance to lincomycin and spiramycin in Staphylococcus aureus. J. molec. Biol. 74, 67–72 (1973b)
Mets, L., Bogorad, L.: Altered chloroplast ribosomal proteins associated with erythromycin-resistant mutants in two genetic systems of Chlamydomonas reinhardi. Proc. nat. Acad. Sci. (Wash.) 69, 3779–3783 (1972)
Sager, R.: Mendelian and nonmendelian inheritance of streptomycin resistance in Chlamydomonas reinhardi. Proc. nat. Acad. Sci. (Wash.) 40, 356–363 (1954)
Author information
Authors and Affiliations
Additional information
This work was supported by the Deutsche Forschungsgemeinschaft.
Rights and permissions
About this article
Cite this article
Spiess, H., Arnold, C.G. Ribosomal proteins in plastids of a mendelian and a nonmendelian streptomycin-resistant mutant of Chlamydomonas reinhardii determined by two-dimensional gel-electrophoresis. Arch. Microbiol. 103, 89–90 (1975). https://doi.org/10.1007/BF00436335
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00436335