Summary
To study the influence of the local base, composition upon UV-induced mutability the reversion frequencies of ten trpA mutants with known codon sequences at position 211 were compared. Comparison of mutant strains reverting by the same base substitution type but with different dipyrimidinic, sequences reveals the mutagenic character of the pyrimidine-pyrimidine (6-4) photoproduct. The codons GAC and GAT, both reverting by AT-GC transitions and AT-CG transversions in the middle position and harboring the dipyrimidinic sequence CT in the opposite strand, differ, in their reversion frequencies sixfold. This difference can only be due to the influence of the different bases in the third codon position upon the mutability of adjacent second bases.
References
Allen MK, Yanofsky C (1963) A biochemical and genetic study of reversion with A-gene A-protein system of Escherichia coli tryptophan synthetase. Genetics 48:1065–1082
Brash EB, Haseltine WA (1982) UV-induced mutation hot-spots occur at DNA damage hotspots. Nature 298:189–192
Coulondre C, Miller JH (1977) Genetic studies of the lac repressor IV. Mutagenic specificity in the lacI gene of Escherichia coli. J Mol Biol 117:577–606
Fowler RG, McGinty L, Mortelmans KE (1981) Mutational specificity of ultraviolet light in Escherichia coli with and without the R plasmid pKM 101. Genetics 99:25–40
Kato T, Shinoura Y, Templin A, Clark AJ (1980) Analysis of ultraviolet light-induced suppressor mutations in the strain of Escherichia coli K-12 AB1157: An implication for molecular mechanisms of UV mutagenesis. Mol Gen Genet 180:283–291
Lippke JA, Gordon LK, Brash DE, Haseltine WA (1981) Distribution of UV light-induced damage in a defined sequence of human DNA: Detection of alkaline-sensitive lesions at pyrimidine nucleoside-cytidine sequences Proc Natl Acad Sci USA 78:3388–3392
Miller JH (1972) Experiments in molecular genetics. Cold Spring Harbor Laboratory, New York
Murgola EJ, Yanofsky C (1974) Selection for new amino acids at position 211 of the tryptophan synthetase α-chain of Escherichia coli. J Mol Biol 86:775–784
Nichols BP, Yanofsky C (1979) Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison. Proc Natl Acad Sci USA 76:5244–5248
Piechocki R, Langhammer R (1980) UV-induced tandem double mutations in the trpA gene of E. coli. Studia Biophysica 80:231–232
Stadler J, Yanofsky C (1959) Studies on a series of tryptophan-independent strains derived from a tryptophan-requiring mutant of Escherichia coli. Genetics 44:105–123
Todd PA, Glickmann BW (1982) Mutational specificity of UV light in Escherichia coli: Indications for a role of DNA secondary structure. Proc Natl Acad Sci USA 79:4123–4127
Vechet B (1974) Some problems in the absolute measurement of germicidal ultraviolet radiation: the use of “pen ray” lamps as a calibration standard. Photochem Photobiol 19:329–335
Vogel HJ, Bonner DM (1956) Acetylornithase, of Escherichia coli: partial purification and some properties. J Biol Chem 218:97–106
Witkin EM (1976) Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriol Rev 40:869–907
Witkin EM, Wermundsen IE (1979) Targeted and untargeted mutagenesis by various inducers of SOS functions in Escherichia coli. Cold Spring Harbor Symp Quant Biol 43:881–886
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Communicated by H. Böhme
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Langhammer, R., Piechocki, R. Comparative analysis of UV-induced mutability of ten different codon units in position 211 of the Escherichia coli trpA gene. Molec. Gen. Genet. 196, 530–532 (1984). https://doi.org/10.1007/BF00436204
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DOI: https://doi.org/10.1007/BF00436204