Molecular and General Genetics MGG

, Volume 196, Issue 3, pp 381–386 | Cite as

Bacteriophage ϕ29 DNA replication in vitro: Participation of the terminal protein and the gene 2 product in elongation

  • Kouji Matsumoto
  • Toshiyuki Saito
  • Chang I Kim
  • Tadahiko Ando
  • Hideo Hirokawa


From ϕ29-infected Bacillus subtilis cells, we have isolated a protein fraction which promotes in vitro replication of ϕ29 DNA. This fraction catalyses both initiation and elongation, indicating that it contains the product of gene 3 (tp: terminal protein) and the product of gene 2 (gp2: probably a DNA polymerase), since initiation requires the two products (Blanco et al. 1983; Matsumoto et al. 1983). The fractions isolated from cells infected with temperature-sensitive (ts) mutants of gene 2 and gene 3 were thermolabile in both the initiation and elongation assays. When the pre-initiated material from the ts fractions of each mutant was heat-inactivated and mixed no complementation, restoring the elongation activity, was found. These results indicate: (i) tp and gp2 participate not only in the initiation but also in the elongation of ϕ29 DNA replication, (ii) they probably function in tight physical association with each other.


Catalysis Bacillus Bacillus Subtilis Protein Fraction Physical Association 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



product of gene 2


terminal protein


DNA with terminal protein covalently linked at both the 5′ ends


2′,3′-dideoxycytidine 5′-triphosphate


2′,3′-dideoxyguanosine 5′ triphosphate


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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Kouji Matsumoto
    • 1
  • Toshiyuki Saito
    • 1
  • Chang I Kim
    • 1
  • Tadahiko Ando
    • 2
  • Hideo Hirokawa
    • 1
  1. 1.Life Science InstituteSophia UniversityTokyoJapan
  2. 2.Department of MicrobiologyRiken Institute (The Institute of Physical and Chemical Research)Wako-shi, SaitamaJapan

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