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Chemical composition of normal and osteoarthrotic cancellous bone of the femoral head

Studies of EDTA extracts and collagenase digests

  • Clinical and Experimental Forum
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Summary

Using EDTA extraction and collagenase digestion, cancellous bone from the femoral heads of ten normal and eight osteoarthrotic individuals was analyzed for its content of collagen, sialoprotein, proteoglycan, and carbohydrate. The EDTA extractability of the matrix proteins of the osteoarthrotic bone was significantly increased (P< 0.001), as was the soluble collagenase-resistant fraction (SCRF). EDTA residues, bone matrix size, the collagenase-resistant fraction (CRF), and the insoluble collagenase-resistant fraction (ICRF) of the osteoarthrotic cases were not different from those of the controls. The amounts of carbohydrate and proteoglycans were considerably elevated in the bone matrix of the osteoarthrotic bone (P<0.01 and P<0.001, respectively). In the EDTA extracts, sialoprotein and proteoglycan contents were found in significant higher amounts (P<0.05 and P<0.01, respectively) in the osteoarthrotic cases. In the SCRF, the hexose and sialic acid contents were higher in the osteoarthrotic bone (P<0.01), while in the ICRF all the analyses were significantly increased in the osteoarthrotic bone (P<0.001). The ratios of collagen to non-collagenaus components were lower in the osteoarthrotic than in normal bones. The quantitative and qualitative variations in cancellous bone proteins from the femoral head in osteoarthrosis found in this study suggest that alterations in subchondral bone play a role in the pathophysiology of cartilage degeneration in osteoarthrosis.

Zusammenfassung

Mittels EDTA Extraktion und Kollagenase-Fermentierung wurde der Gehalt an Kollagen, Sialoprotein, Proteoglycan und Karbohydrat im Knochenmaterial der Hüftköpfe von 10 normalen und 8 osteoarthrotischen Personen analysiert. Die EDTA Extrahierbarkeit der Matrixproteine des osteoarthrotischen Knochens war signifikant gestiegen (P>0,001) ebenso wie der lösliche Kollagenase resistente Teil (LKRT). EDTA Reste, die Größe der Knochenmatrix, der Kollagenase resistente Teil (KRT) and der unlösliche Kollagenase resistente Teil (UKRT) der osteoarthrotischen Hüftköpfe unterschieden sich nicht von den Kontrollfällen. Die Mengen von Karbohydrat und Proteoglycan waren deutlich erhöht in der Knochenmatrix der osteoarthrotischen Knochen (P<0,01 bzw. P<0,001). In den EDTA Extrakten war der Gehalt an Sialoprotein und Proteoglycan bei den osteoarthrotischen Hüftköpfen deutlich höher (P< 0,05 bzw. P< 0,01). Im LKRT war der Gehalt an Hexose and sialischer Säure höher im osteoarthrotischen Knochen (P<0,01), während im UKRT alle Analysen deutlich h6her waren im osteoarthrotischen Knochen (P<0,001). Das Verhältnis von Kollagen zum kollagefreien Anteil war niedriger im osteoarthrotischen als im normalen Knochen. Quantitative and qualitative Veränderungen in Knochenproteinen des osteoarthrotischen Hüftkopfes, wie sie die vorliegende Studie ausweist, lassen vermuten, daß Veranderungen im subchondralen Knochen eine wichtige Rolle spielen in der Pathophysiologie der Degeneration des Gelenkknorpels bei Osteoarthrosis.

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Authors and Affiliations

  1. Arthritis and Metabolic Bone Disease Research Unit, K. U. Leuven, Universitair Ziekenhuis, B-3041, Pellenberg, Belgium

    J. M. Mbuyi-Muamba &  J. Dequeker

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  1. J. M. Mbuyi-Muamba
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  2. J. Dequeker
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Mbuyi-Muamba, J.M., Dequeker, J. Chemical composition of normal and osteoarthrotic cancellous bone of the femoral head. Arch. Orth. Traum. Surg. 102, 267–272 (1984). https://doi.org/10.1007/BF00436142

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  • DOI: https://doi.org/10.1007/BF00436142

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