Summary
To investigate the extent of local penetration of antibiotic into the bone, a single dose of 10 mg/kg gentamicin sulphate was injected over the midshaft of the left femur in 12 young and 14 old rats. In some of the rats the injected gentamicin was dissolved in saline while in the others it was dissolved in dimethylsulphoxide (DMSO). Up to 120 minutes after the injection gentamicin concentrations were measured by the fluoroimmunoassay method in the serum, cortex and spongiosa of both femors. In young rats gentamicin was shown to have penetrated directly into the bone and its presence was limited to the femoral cortex. In the old rats, on the other hand hematogenic was found to be the antibiotic spread in the femors. No significant difference was observed between DMSO and saline as solvents in facilitating gentamicin penetration into the bone or capillaries in either age group.
Zusammenfassung
Um das Ausmaß der lokalen Penetration eines Antibiotikums in den Knochen zu untersuchen, wurde eine Einzeldosis von 10 mg/kg Gentamycinsulfat an die Schaftmitte des linken Femur bei 12 jungen und 14 alten Ratten injiziert. Bei einem Teil der Ratten wurde das Gentamycin in physiologischer Kochsalzlösung und bei den anderen in Dimethyl-Sulfoxyd (DMSO) verdünnt. Bis zu 120 Minuten nach der Injektion wurde die Gentamycin-Konzentration im Serum und in der Kortikalis und Spongiosa beider Femora mit der Fluorimmunoanalyse untersucht. Bei jungen Ratten war das Gentamycin direkt in den Knochen eingedrungen und fand sich ausschließlich in der Kortikalis. Bei den alten Ratten wurde das Antibiotikum auf dem Blutwege verteilt. Bei den Verdünnungsmitteln (DMSO und physiologische Kochsalzlösung) wurde hinsichtlich der Verteilung des Antibioticums in Knochen und Kapillaren bei beiden Altersgruppen kein Unterschied festgestellt.
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Mirovsky, Y., Halperin, N. & Copeliovitch, L. Local penetration of gentamicin into the femoral cortex of rats. Arch. Orth. Traum. Surg. 102, 238–241 (1984). https://doi.org/10.1007/BF00436136
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DOI: https://doi.org/10.1007/BF00436136