Acid and alkali as aids in differentiation of pathogenic Nocardia and for their isolation from clinical specimens
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The tolerance of Nocardia brasiliensis, Nocardia asteroides and Nocardia caviae to different concentrations of acids and alkalis was studied in this work. The purpose was to determine their use: (a) in the differentiation of species; (b) for decontamination of clinical materials during the isolation of pathogenic Nocardia. The results showed (1) that both 2 M NH3 and 0.05 M H2SO4 solutions are useful for differentiating species. They can also be used as a complementary method for identification. (2) That both 1 M NH3 and 0.05 M H2SO4 solutions were useful for decontamination of clinical materials during the isolation of pathogenic Nocardia.
Key wordsAcid alkali decontamination differentiation Nocardia pathogenic
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