Summary
The size of the transcriptional unit of the structural genes for three galactose-metabolizing enzymes which form a cluster on chromosome II in Saccharomyces cerevisiae was studied by the ultraviolet light (UV)-mapping technique. Thus the size of the primary transcripts of GAL7 for galactose-1-phosphate uridylyl transferase, GAL10 for uridine diphosphoglucose 4-epimerase, or GAL1 for galactokinase were estimated to be 0.81 x 106, 1.1 x 106, or 1.3 x 106 respectively. In the light of these data together with the known directions of transcription of the genes, we concluded that each of three genes was transcribed from its own promoter.
Similar content being viewed by others
References
Bassel J, Mortimer B (1971) J Bacteriol 108:179–183
Buttin G (1963) J Mol Biol 7:183–205
Carlson J O, Pettijohn D E (1979) J Mol Biol 132:141–161
Fukasawa T, Obonai K, Segawa T, Nogi Y (1980) J Biol Chem 255:2705–2707
Giorno R, Sauerbier W (1976) Cell 9:775–783
Hackett P B, Sauerbier W (1975) J Mol Biol 91:235–256
Hackett P B, Traub P, Gallwith D (1978) J Mol Biol 126:619–635
Hartwell L H (1967) J Bacteriol 93:1162–1670
Holland J P, Holland M J (1979) J Biol Chem 254:9839–9845
Hopper J E, Broach J R, Rowe L B (1978) Proc Nat Acad Sci USA 75:2878–2882
Hopper J E, Rowe L B (1978) J Biol Chem 253:7566–7569
Kalckar H M (1958) Adv Enzymol 20:111–134
Matsumoto K, Toh-e A, Oshima Y (1978) J Bacteriol 134:446–457
Michalke H, Bremer H (1969) J Mol Biol 41:1–23
Montgomery D L, Leung D W, Smith M, Shalit P, Faye G, Hall B D (1980) Proc Nat Acad Sci USA 77:541–545
Mortimer R K, Hawthorne D C (1969) Yeast genetics, In: Rose A H, Harrison J S (eds) The yeasts, vol l, New York, Academic Press, p 385
Nakai S, Matsumoto S (1967) Mutat Res 4:129–136
Nath K, Bollon A P (1977) J Biol Chem 252:6562–6571
Nikolaev N, Georgiev O I, Venkov P V, Hadjiolov A A (1979) J Mol Biol 127:297–308
Nogi Y, Matsumoto K, Toh-e A, Oshima Y (1977) Mol Gen Genet 152:137–144
Perlman D, Hopper J E (1979) Cell 16:89–95
Prakash L (1975) J Mol Biol 98:781–795
Saedler H, Gullon A, Fiethen L, Starlinger P (1968) Mol Gen Genet 102:79–88
Sauerbier W, Millette R L, Hackett P B (1970) Biochim Biophys Acta 209:368–386
Schell M A, Wilson D B (1977) J Biol Chem 252:1162–1166
Schell M A, Wilson D B (1979) J Biol Chem 254:3531–3536
Segawa T, Fukasawa T (1979) J Biol Chem 254:10707–10709
Smith M, Leung D W, Gillam S, Astell C R, Montgomery D L, Hall B D (1979) Cell 16:753–761
St John T P, Davis R W (1979) Cell 16:443–452
Udem S A, Warner J R (1972) J Mol Biol 65:227–242
Unrau P, Wheatcroft R, Cox B S (1971) Mol Gen Genet 113:359–362
Waters R, Moustacchi E (1974) Biochim Biophys Acta 353:407–419
Wilson D (1965) Anal Biochem 10:472–478
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Segawa, T., Fukasawa, T. Transcriptional units of GAL genes in Saccharomyces cerevisiae determined by ultraviolet light mapping. Curr Genet 2, 223–228 (1980). https://doi.org/10.1007/BF00435690
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00435690